Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression inBacillus subtilis

2018 ◽  
Vol 7 (7) ◽  
pp. 1785-1797 ◽  
Author(s):  
Dingyu Liu ◽  
Zhitao Mao ◽  
Jiaxin Guo ◽  
Leyi Wei ◽  
Hongwu Ma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Model Based ◽  
Promoter Library ◽  
Construction Model ◽  
Model Based Analysis

Construction and Characterization of a Gradient Strength Promoter Library for Fine-Tuned Gene Expression in Bacillus licheniformis

2021 ◽  
Vol 10 (9) ◽  
pp. 2331-2339
Author(s):  
Yi Rao ◽  
Peifen Li ◽  
Xinxin Xie ◽  
Jiemin Li ◽  
Yongqing Liao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bacillus Licheniformis ◽  
Gradient Strength ◽  
Promoter Library

scholarly journals Characterization of the hemodynamic modes associated with interictal epileptic activity using a deformable model-based analysis of combined EEG and functional MRI recordings

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Frédéric Grouiller ◽  
Laurent Vercueil ◽  
Alexandre Krainik ◽  
Christoph Segebarth ◽  
Philippe Kahane ◽  
...  
Keyword(s):  
Functional Mri ◽  
Epileptic Activity ◽  
Deformable Model ◽  
Model Based ◽  
Model Based Analysis

scholarly journals GAPPromoter Library for Fine-Tuning of Gene Expression in Pichia pastoris

2011 ◽  
Vol 77 (11) ◽  
pp. 3600-3608 ◽  
Author(s):  
Xiulin Qin ◽  
Jiangchao Qian ◽  
Gaofeng Yao ◽  
Yingping Zhuang ◽  
Siliang Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pichia Pastoris ◽  
Fine Tuning ◽  
Promoter Strength ◽  
Control Of Gene Expression ◽  
Content Type ◽  
Expression Levels ◽  
Precise Control ◽  
Promoter Library

ABSTRACTA library of engineered promoters of various strengths is a useful genetic tool that enables the fine-tuning and precise control of gene expression across a continuum of broad expression levels. The methylotrophic yeastPichia pastorisis a well-established expression host with a large academic and industrial user base. To facilitate manipulation of gene expression spanning a wide dynamic range inP. pastoris, we created a functional promoter library through mutagenesis of the constitutiveGAPpromoter. Using yeast-enhanced green fluorescent protein (yEGFP) as the reporter, 33 mutants were chosen to form the functional promoter library. The 33 mutants spanned an activity range between ∼0.6% and 19.6-fold of the wild-type promoter activity with an almost linear fluorescence intensity distribution. After an extensive characterization of the library, the broader applicability of the results obtained with the yEGFP reporter was confirmed using two additional reporters (β-galactosidase and methionine adenosyltransferase [MAT]) at the transcription and enzyme activity levels. Furthermore, the utility of the promoter library was tested by investigating the influence of heterologous MAT gene expression levels on cell growth andS-adenosylmethionine (SAM) production. The extensive characterization of the promoter strength enabled identification of the optimal MAT activity (around 1.05 U/mg of protein) to obtain maximal volumetric SAM production. The promoter library permits precise control of gene expression and quantitative assessment that correlates gene expression level with physiologic parameters. Thus, it is a useful toolbox for both basic and applied research inP. pastoris.

scholarly journals Model-Based Characterization of Inflammatory Gene Expression Patterns of Activated Macrophages

2016 ◽  
Vol 12 (7) ◽  
pp. e1005018 ◽  
Author(s):  
Julia Rex ◽  
Ute Albrecht ◽  
Christian Ehlting ◽  
Maria Thomas ◽  
Ulrich M. Zanger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Activated Macrophages ◽  
Inflammatory Gene Expression ◽  
Inflammatory Gene ◽  
Model Based

scholarly journals Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering

2007 ◽  
Vol 7 (1) ◽  
pp. 34 ◽  
Author(s):  
Marjan De Mey ◽  
Jo Maertens ◽  
Gaspard J Lequeux ◽  
Wim K Soetaert ◽  
Erick J Vandamme
Keyword(s):  
Metabolic Engineering ◽  
E Coli ◽  
Model Based ◽  
Promoter Library ◽  
Indispensable Tool ◽  
Model Based Analysis

Characterization of tissue response to radiofrequency ablation using 3D model-based analysis of interventional MR images

2003 ◽  
Author(s):  
Brent D. Weinberg ◽  
Roee S. Lazebnik ◽  
Michael S. Breen ◽  
Jonathan S. Lewin ◽  
David L. Wilson
Keyword(s):  
Radiofrequency Ablation ◽  
3D Model ◽  
Tissue Response ◽  
Mr Images ◽  
Model Based ◽  
Interventional Mr ◽  
Model Based Analysis
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