scholarly journals Tuning of Recombinant Protein Expression in Escherichia coli by Manipulating Transcription, Translation Initiation Rates, and Incorporation of Noncanonical Amino Acids

2017 ◽  
Vol 6 (6) ◽  
pp. 1076-1085 ◽  
Author(s):  
Orr Schlesinger ◽  
Yonatan Chemla ◽  
Mathias Heltberg ◽  
Eden Ozer ◽  
Ryan Marshall ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Recombinant Protein ◽  
Protein Expression ◽  
Translation Initiation ◽  
Recombinant Protein Expression ◽  
Noncanonical Amino Acids ◽  
Expression In Escherichia Coli

Recombinant Protein Expression in Escherichia coli (E.coli): What We Need to Know

2018 ◽  
Vol 24 (6) ◽  
pp. 718-725 ◽  
Author(s):  
Seyed Mohammad Gheibi Hayat ◽  
Najmeh Farahani ◽  
Behrouz Golichenari ◽  
Amirhossein Sahebkar
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Protein Expression ◽  
Recombinant Proteins ◽  
Recombinant Protein Expression ◽  
Culture Conditions ◽  
Strain Vector ◽  
Factors Affecting ◽  
Expression In Escherichia Coli ◽  
Pros And Cons

Background: Host, vector, and culture conditions (including cultivation media) are considered among the three main elements contributing to a successful production of recombinant proteins. Accordingly, one of the most common hosts to produce recombinant therapeutic proteins is Escherichia coli. Methodology: A comprehensive literature review was performed to identify important factors affecting production of recombinant proteins in Escherichia coli. Results: Escherichia coli is taken into account as the easiest, quickest, and cheapest host with a fully known genome. Thus, numerous modifications have been carried out on Escherichia coli to optimize it as a good candidate for protein expression and; as a result, several engineered strains of Escherichia coli have been designed. In general; host strain, vector, and cultivation parameters are recognized as crucial ones determining success of recombinant protein expression in Escherichia coli. In this review, the role of host, vector, and culture conditions along with current pros and cons of different types of these factors leading to success of recombinant protein expression in Escherichia coli were discussed. Conclusion: Successful protein expression in Escherichia coli necessitates a broad knowledge about physicochemical properties of recombinant proteins, selection among common strains of Escherichia coli and vectors, as well as factors related to media including time, temperature, and inducer.

scholarly journals A novel knock out strategy to enhance recombinant protein expression in Escherichia coli

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Ashish K. Sharma ◽  
Esha Shukla ◽  
Deepak S. Janoti ◽  
Krishna J. Mukherjee ◽  
Joseph Shiloach
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Protein Expression ◽  
Recombinant Protein Expression ◽  
Knock Out ◽  
Expression In Escherichia Coli
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