Designing Single-Component Optogenetic Membrane Recruitment Systems: The Rho-Family GTPase Signaling Toolbox

2022 ◽  
Author(s):  
Erin E. Berlew ◽  
Keisuke Yamada ◽  
Ivan A. Kuznetsov ◽  
Eleanor A. Rand ◽  
Chandler C. Ochs ◽  
...  
Keyword(s):  
Single Component ◽  
Membrane Recruitment ◽  
Rho Family

scholarly journals Single-component optogenetic tools for inducible RhoA GTPase signaling

2021 ◽  
Author(s):  
Erin E. Berlew ◽  
Ivan A. Kuznetsov ◽  
Keisuke Yamada ◽  
Lukasz J. Bugaj ◽  
Joel D. Boerckel ◽  
...  
Keyword(s):  
Transcriptional Activity ◽  
Cell Polarization ◽  
Single Component ◽  
Membrane Recruitment ◽  
Actin Organization ◽  
Precise Control ◽  
Binding Partners ◽  
Actomyosin Contractility ◽  
Rhoa Gtpase ◽  
Rhoa Signaling

ABSTRACTWe created optogenetic tools to control RhoA GTPase, a central regulator of actin organization and actomyosin contractility. RhoA GTPase, or its upstream activating GEF effectors, were fused to BcLOV4, a photoreceptor that can be dynamically recruited to the plasma membrane by a light-regulated protein-lipid electrostatic interaction with the inner leaflet. Direct membrane recruitment of these effectors induced potent contractile signaling sufficient to separate adherens junctions in response to as little as one pulse of blue light. Cytoskeletal morphology changes were dependent on the alignment of the spatially patterned stimulation with the underlying cell polarization. RhoA-mediated cytoskeletal activation induced YAP nuclear localization within minutes and subsequent mechanotransduction, verified by YAP-TEAD transcriptional activity. These single-component tools, which do not require protein binding partners, offer spatiotemporally precise control over RhoA signaling that will advance the study of its diverse regulatory roles in cell migration, morphogenesis, and cell cycle maintenance.

scholarly journals Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation

2020 ◽  
Vol 19 (3) ◽  
pp. 353-361 ◽  
Author(s):  
Erin E. Berlew ◽  
Ivan A. Kuznetsov ◽  
Keisuke Yamada ◽  
Lukasz J. Bugaj ◽  
Brian Y. Chow
Keyword(s):  
Actin Polymerization ◽  
Membrane Lipid ◽  
Lipid Binding ◽  
Single Component ◽  
Dynamic Membrane ◽  
Membrane Recruitment ◽  
Lamellipodia Formation

We report the construction of a single-component optogenetic Rac1 (opto-Rac1) to control actin polymerization by dynamic membrane recruitment.

The role of Rho-family GTPases in human glioblastomas

2004 ◽  
Vol 31 (S 1) ◽  
Author(s):  
S Seta ◽  
M Herr ◽  
S Horn ◽  
D Koch ◽  
T Vogt ◽  
...  
Keyword(s):  
Rho Family Gtpases ◽  
Rho Family

Boiling of single component liquids: Basic processes

2017 ◽  
Author(s):  
John G. Collier ◽  
V.V. Wadekar
Keyword(s):  
Single Component

ERROR LOCALIZATION DIAGNOSTIC MODEL OPTIMIZATION IN DIGITAL STATE MACHINES NETWORKS

2019 ◽  
Author(s):  
Irina Bystrova ◽  
E. Danil'chuk ◽  
Boris Podkopaev
Keyword(s):  
Single Component ◽  
Diagnostic Model ◽  
State Machines ◽  
Model Optimization ◽  
Error Localization ◽  
Diagnostic Models

The problem of constructing a diagnostic model for a network S consisting of a number of digital automata is considered, provided that the diagnostic models of all network components are known. It is assumed that these models are given by systems of logical equations, and the errors to be detected are localized in any but a single component of the network.

Sign in / Sign up

Export Citation Format

Share Document