Development of a Highly Efficient Copper-Inducible GAL Regulation System (CuIGR) in Saccharomyces cerevisiae

2021 ◽  
Author(s):  
Pingping Zhou ◽  
Xin Fang ◽  
Nannan Xu ◽  
Zhen Yao ◽  
Wenping Xie ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Regulation System ◽  
Highly Efficient

scholarly journals Cultivation of Saccharomyces cerevisiae with Feedback Regulation of Glucose Concentration Controlled by Optical Fiber Glucose Sensor

Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 565
Author(s):  
Lucie Koštejnová ◽  
Jakub Ondráček ◽  
Petra Majerová ◽  
Martin Koštejn ◽  
Gabriela Kuncová ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Optical Fiber ◽  
Glucose Concentration ◽  
Glucose Sensor ◽  
Ruthenium Complex ◽  
Feedback Regulation ◽  
Regulation System ◽  
Sensitive Layer ◽  
Average Deviation ◽  
Glucose Content

Glucose belongs among the most important substances in both physiology and industry. Current food and biotechnology praxis emphasizes its on-line continuous monitoring and regulation. These provoke increasing demand for systems, which enable fast detection and regulation of deviations from desired glucose concentration. We demonstrated control of glucose concentration by feedback regulation equipped with in situ optical fiber glucose sensor. The sensitive layer of the sensor comprises oxygen-dependent ruthenium complex and preimmobilized glucose oxidase both entrapped in organic–inorganic polymer ORMOCER®. The sensor was placed in the laboratory bioreactor (volume 5 L) to demonstrate both regulations: the control of low levels of glucose concentrations (0.4 and 0.1 mM) and maintenance of the glucose concentration (between 2 and 3.5 mM) during stationary phase of cultivation of Saccharomyces cerevisiae. Response times did not exceed 6 min (average 4 min) with average deviation of 4%. Due to these regulation characteristics together with durable and long-lasting (≥2 month) sensitive layer, this feedback regulation system might find applications in various biotechnological processes such as production of low glucose content beverages.

An Improved Assay of UV-induced Thymine-Containing Dimers in Saccharomyces cerevisiae

1975 ◽  
Vol 30 (11-12) ◽  
pp. 804-810 ◽  
Author(s):  
W. W. Fäth ◽  
M. Brendel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Quantitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Highly Efficient ◽  
Layer Chromatography ◽  
Specific Labelling

Abstract The method for assaying thymine-containing dimers in yeast is based on highly efficient ([3H]-deoxythymidine-5′-monophosphate) DNA-specific labelling and employs ascending thin layer chromatography. It allows satisfactory quantitative analysis down to UV-doses of 500 erg/mm2.

scholarly journals INHIBITION OF GROWTH BY AMBER SUPPRESSORS IN YEAST

Genetics ◽  
1976 ◽  
Vol 82 (2) ◽  
pp. 233-249
Author(s):  
Susan W Liebman ◽  
Fred Sherman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Growth Rates ◽  
Nutrient Medium ◽  
Normal Growth ◽  
Yeast Saccharomyces Cerevisiae ◽  
Highly Efficient ◽  
Critical Range ◽  
Inhibition Of Growth ◽  
Amber Suppressor

ABSTRACT Strains of the yeast Saccharomyces cerevisiae that contain highly efficient amber (UAG) suppressors grow poorly on nutrient medium, while normal or nearly normal growth rates are observed when these strains lose the suppressors or when the suppressors are mutated to lower efficiencies. The different growth rates account for the accumulation of mutants with lowered efficiencies in cultures of strains with highly efficient amber suppressors. Genetic analyses indicate that one of the mutations with a lowered efficiency of suppression is caused by an intragenic mutation of the amber suppressor. The inhibition of growth caused by excessive suppression is expected to be exacerbated when appropriate suppressors are combined together in haploid cells if two suppressors act with a greater efficiency than a single suppressor. Such retardation of growth is observed with combinations of two UAA (ochre) suppressors (Gilmore 1967) and with combinations of two UAG suppressors when the efficiencies of each of the suppressors are within a critical range. In contrast, combinations of a UAA suppressor and a UAG suppressor do not affect growth rate. Apparently while either excessive UAA or excessive UAG suppression is deleterious to yeast, a moderate level of simultaneous UAA and UAG suppression is not.

Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol

2012 ◽  
Vol 29 (3) ◽  
pp. 379-386 ◽  
Author(s):  
Suthee Benjaphokee ◽  
Daisuke Hasegawa ◽  
Daiki Yokota ◽  
Thipa Asvarak ◽  
Choowong Auesukaree ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Temperature ◽  
Stress Tolerance ◽  
Bioethanol Production ◽  
Multiple Stress ◽  
Highly Efficient ◽  
Multiple Stress Tolerance

scholarly journals Combinatorial Design of a Highly Efficient Xylose-Utilizing Pathway in Saccharomyces cerevisiae for the Production of Cellulosic Biofuels

2012 ◽  
Vol 79 (3) ◽  
pp. 931-941 ◽  
Author(s):  
Byoungjin Kim ◽  
Jing Du ◽  
Dawn T. Eriksen ◽  
Huimin Zhao
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Flux ◽  
Value Added ◽  
Major Product ◽  
Combinatorial Design ◽  
Biofuel Production ◽  
Pathway Engineering ◽  
Full Potential ◽  
Xylose Utilization ◽  
Highly Efficient

ABSTRACTBalancing the flux of a heterologous metabolic pathway by tuning the expression and properties of the pathway enzymes is difficult, but it is critical to realizing the full potential of microbial biotechnology. One prominent example is the metabolic engineering of aSaccharomyces cerevisiaestrain harboring a heterologous xylose-utilizing pathway for cellulosic-biofuel production, which remains a challenge even after decades of research. Here, we developed a combinatorial pathway-engineering approach to rapidly create a highly efficient xylose-utilizing pathway for ethanol production by exploring various combinations of enzyme homologues with different properties. A library of more than 8,000 xylose utilization pathways was generated using DNA assembler, followed by multitiered screening, which led to the identification of a number of strain-specific combinations of the enzymes for efficient conversion of xylose to ethanol. The balancing of metabolic flux through the xylose utilization pathway was demonstrated by a complete reversal of the major product from xylitol to ethanol with a similar yield and total by-product formation as low as 0.06 g/g xylose without compromising cell growth. The results also suggested that an optimal enzyme combination depends on not only the genotype/phenotype of the host strain, but also the sugar composition of the fermentation medium. This combinatorial approach should be applicable to any heterologous pathway and will be instrumental in the optimization of industrial production of value-added products.

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