scholarly journals Biosynthesis of Poly(3HB-co-3HP) with Variable Monomer Composition in Recombinant Cupriavidus necator H16

2021 ◽  
Author(s):  
Callum McGregor ◽  
Nigel P. Minton ◽  
Katalin Kovács
Keyword(s):  
Cupriavidus Necator ◽  
Monomer Composition ◽  
Cupriavidus Necator H16

scholarly journals Complete Genome Sequence of Cupriavidus necator H16 (DSM 428)

2019 ◽  
Vol 8 (37) ◽  
Author(s):  
Gareth T. Little ◽  
Muhammad Ehsaan ◽  
Christian Arenas-López ◽  
Kamran Jawed ◽  
Klaus Winzer ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Cupriavidus Necator ◽  
Rrna Genes ◽  
Trna Genes ◽  
Protein Coding ◽  
Content Type ◽  
Protein Coding Genes ◽  
Cupriavidus Necator H16

The hydrogen-utilizing strain Cupriavidus necator H16 (DSM 428) was sequenced using a combination of PacBio and Illumina sequencing. Annotation of this strain reveals 6,543 protein-coding genes, 263 pseudogenes, 64 tRNA genes, and 15 rRNA genes.

scholarly journals Adaptive Laboratory Evolution of Cupriavidus necator H16 for Carbon Co-Utilization with Glycerol

2019 ◽  
Vol 20 (22) ◽  
pp. 5737 ◽  
Author(s):  
Miriam González-Villanueva ◽  
Hemanshi Galaiya ◽  
Paul Staniland ◽  
Jessica Staniland ◽  
Ian Savill ◽  
...  
Keyword(s):  
Type Strain ◽  
Wild Type Strain ◽  
Carbon Sources ◽  
Strain Engineering ◽  
Cupriavidus Necator ◽  
Superior Performance ◽  
Wild Type ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
Cupriavidus Necator H16

Cupriavidus necator H16 is a non-pathogenic Gram-negative betaproteobacterium that can utilize a broad range of renewable heterotrophic resources to produce chemicals ranging from polyhydroxybutyrate (biopolymer) to alcohols, alkanes, and alkenes. However, C. necator H16 utilizes carbon sources to different efficiency, for example its growth in glycerol is 11.4 times slower than a favorable substrate like gluconate. This work used adaptive laboratory evolution to enhance the glycerol assimilation in C. necator H16 and identified a variant (v6C6) that can co-utilize gluconate and glycerol. The v6C6 variant has a specific growth rate in glycerol 9.5 times faster than the wild-type strain and grows faster in mixed gluconate–glycerol carbon sources compared to gluconate alone. It also accumulated more PHB when cultivated in glycerol medium compared to gluconate medium while the inverse is true for the wild-type strain. Through genome sequencing and expression studies, glycerol kinase was identified as the key enzyme for its improved glycerol utilization. The superior performance of v6C6 in assimilating pure glycerol was extended to crude glycerol (sweetwater) from an industrial fat splitting process. These results highlight the robustness of adaptive laboratory evolution for strain engineering and the versatility and potential of C. necator H16 for industrial waste glycerol valorization.

scholarly journals Cupriavidus necator H16 Uses Flavocytochrome c Sulfide Dehydrogenase To Oxidize Self-Produced and Added Sulfide

2017 ◽  
Vol 83 (22) ◽  
Author(s):  
Chuanjuan Lü ◽  
Yongzhen Xia ◽  
Daixi Liu ◽  
Rui Zhao ◽  
Rui Gao ◽  
...  
Keyword(s):  
Gas Phase ◽  
Heterotrophic Bacteria ◽  
Sulfide Oxidation ◽  
Cupriavidus Necator ◽  
Content Type ◽  
Sulfide:Quinone Oxidoreductase ◽  
Transport Chain ◽  
Genes Encoding ◽  
Cupriavidus Necator H16 ◽  
Chemolithotrophic Bacteria

ABSTRACT Production of sulfide (H2S, HS−, and S2−) by heterotrophic bacteria during aerobic growth is a common phenomenon. Some bacteria with sulfide:quinone oxidoreductase (SQR) and persulfide dioxygenase (PDO) can oxidize self-produced sulfide to sulfite and thiosulfate, but other bacteria without these enzymes release sulfide into the medium, from which H2S can volatilize into the gas phase. Here, we report that Cupriavidus necator H16, with the fccA and fccB genes encoding flavocytochrome c sulfide dehydrogenases (FCSDs), also oxidized self-produced H2S. A mutant in which fccA and fccB were deleted accumulated and released H2S. When fccA and fccB were expressed in Pseudomonas aeruginosa strain Pa3K with deletions of its sqr and pdo genes, the recombinant rapidly oxidized sulfide to sulfane sulfur. When PDO was also cloned into the recombinant, the recombinant with both FCSD and PDO oxidized sulfide to sulfite and thiosulfate. Thus, the proposed pathway is similar to the pathway catalyzed by SQR and PDO, in which FCSD oxidizes sulfide to polysulfide, polysulfide spontaneously reacts with reduced glutathione (GSH) to produce glutathione persulfide (GSSH), and PDO oxidizes GSSH to sulfite, which chemically reacts with polysulfide to produce thiosulfate. About 20.6% of sequenced bacterial genomes contain SQR, and only 3.9% contain FCSD. This is not a surprise, since SQR is more efficient in conserving energy because it passes electrons from sulfide oxidation into the electron transport chain at the quinone level, while FCSD passes electrons to cytochrome c. The transport of electrons from the latter to O2 conserves less energy. FCSDs are grouped into three subgroups, well conserved at the taxonomic level. Thus, our data show the diversity in sulfide oxidation by heterotrophic bacteria. IMPORTANCE Heterotrophic bacteria with SQR and PDO can oxidize self-produced sulfide and do not release H2S into the gas phase. C. necator H16 has FCSD but not SQR, and it does not release H2S. We confirmed that the bacterium used FCSD for the oxidation of self-produced sulfide. The bacterium also oxidized added sulfide. The common presence of SQRs, FCSDs, and PDOs in heterotrophic bacteria suggests the significant role of heterotrophic bacteria in sulfide oxidation, participating in sulfur biogeochemical cycling. Further, FCSDs have been identified in anaerobic photosynthetic bacteria and chemolithotrophic bacteria, but their physiological roles are unknown. We showed that heterotrophic bacteria use FCSDs to oxidize self-produced sulfide and extraneous sulfide, and they may be used for H2S bioremediation.

Raman Spectral Profiles of PHB Synthesis by Cupriavidus necator H16 at Different Fructose Levels

2015 ◽  
Vol 42 (3) ◽  
pp. 0315003
Author(s):  
覃赵军 Qin Zhaojun ◽  
彭立新 Peng Lixin ◽  
竺利波 Zhu Libo ◽  
宋水山 Song Shuishan ◽  
刘军贤 Liu Junxian ◽  
...  
Keyword(s):  
Cupriavidus Necator ◽  
Cupriavidus Necator H16 ◽  
Spectral Profiles ◽  
Raman Spectral

Application of thermo-separating aqueous two-phase system in extractive bioconversion of polyhydroxyalkanoates by Cupriavidus necator H16

2019 ◽  
Vol 287 ◽  
pp. 121474 ◽  
Author(s):  
Yoong Kit Leong ◽  
Pau-Loke Show ◽  
John Chi-Wei Lan ◽  
Rambabu Krishnamoorthy ◽  
Dinh-Toi Chu ◽  
...  
Keyword(s):  
Cupriavidus Necator ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Cupriavidus Necator H16 ◽  
Extractive Bioconversion

scholarly journals Probing the Kinetic Anabolism of Poly-Beta-Hydroxybutyrate in Cupriavidus necator H16 Using Single-Cell Raman Spectroscopy

Sensors ◽  
2016 ◽  
Vol 16 (8) ◽  
pp. 1257 ◽  
Author(s):  
Zhanhua Tao ◽  
Lixin Peng ◽  
Pengfei Zhang ◽  
Yong-Qing Li ◽  
Guiwen Wang
Keyword(s):  
Raman Spectroscopy ◽  
Single Cell ◽  
Cupriavidus Necator ◽  
Cupriavidus Necator H16 ◽  
Beta Hydroxybutyrate

Effect of ethanol and hydrogen peroxide on poly(3-hydroxybutyrate) biosynthetic pathway in Cupriavidus necator H16

2010 ◽  
Vol 26 (7) ◽  
pp. 1261-1267 ◽  
Author(s):  
Stanislav Obruca ◽  
Ivana Marova ◽  
Marie Stankova ◽  
Ludmila Mravcova ◽  
Zdenek Svoboda
Keyword(s):  
Hydrogen Peroxide ◽  
Biosynthetic Pathway ◽  
Cupriavidus Necator ◽  
Cupriavidus Necator H16
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