A Ratiometric Dual Color Luciferase Reporter for Fast Characterization of Transcriptional Regulatory Elements in Plants

2021 ◽  
Author(s):  
Eduardo González-Grandío ◽  
Gozde S. Demirer ◽  
Wenhe Ma ◽  
Siobhan Brady ◽  
Markita P. Landry
Keyword(s):  
Regulatory Elements ◽  
Luciferase Reporter ◽  
Transcriptional Regulatory Elements ◽  
Dual Color ◽  
Transcriptional Regulatory

scholarly journals Characterization of Transcriptional Regulatory Elements in the Promoter Region of the Murine Blood Coagulation Factor VII Gene

1998 ◽  
Vol 273 (4) ◽  
pp. 2277-2287 ◽  
Author(s):  
Daniel R. Stauffer ◽  
Beatrice N. Chukwumezie ◽  
Julie A. Wilberding ◽  
Elliot D. Rosen ◽  
Francis J. Castellino
Keyword(s):  
Blood Coagulation ◽  
Promoter Region ◽  
Coagulation Factor ◽  
Regulatory Elements ◽  
Factor Vii ◽  
Transcriptional Regulatory Elements ◽  
Coagulation Factor Vii ◽  
Transcriptional Regulatory

scholarly journals A ratiometric dual color luciferase reporter for fast characterization of transcriptional regulatory elements

2021 ◽  
Author(s):  
Eduardo González-Grandío ◽  
Gözde Sultan Demirer ◽  
Wenhe Ma ◽  
Siobhan M Brady ◽  
Markita P Landry
Keyword(s):  
Gene Expression ◽  
Synthetic Biology ◽  
Plant Traits ◽  
Regulatory Elements ◽  
Luciferase Reporter ◽  
Control Of Gene Expression ◽  
Transcriptional Reporter ◽  
Dual Color ◽  
Reporter Assays

Plant synthetic biology requires precise characterization of genetic elements to construct complex genetic circuits that can improve plant traits or confer them new characteristics. Transcriptional reporter assays are essential to quantify the effect of gene expression regulator elements. Therefore, transcriptional reporter systems are a key tool in understanding control of gene expression in biology. In this work we construct and characterize a dual-color luciferase ratiometric reporter system that possesses several advantages over currently used reporters. It is ratiometric, reducing variability and increasing consistency between experiments; it is fast, as both reporters can be measured at the same time in a single reaction, and it is cheaper to perform than current dual-luciferase reporter assays. We have validated our system quantifying the transcriptional capability of a panel of promoters and terminators commonly used in synthetic biology with a broad range of expression magnitudes, and in a biologically relevant system, nitrate response.

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