Inducible Population Quality Control of Engineered Bacillus subtilis for Improved N-Acetylneuraminic Acid Biosynthesis

2021 ◽  
Author(s):  
Yanting Cao ◽  
Rongzhen Tian ◽  
Xueqin Lv ◽  
Jianghua Li ◽  
Long Liu ◽  
...  
Keyword(s):  
Quality Control ◽  
Bacillus Subtilis ◽  
Acid Biosynthesis ◽  
Acetylneuraminic Acid ◽  
Population Quality

scholarly journals A novel two-gene requirement for the octanoyltransfer reaction of Bacillus subtilis lipoic acid biosynthesis

2011 ◽  
Vol 80 (2) ◽  
pp. 335-349 ◽  
Author(s):  
Natalia Martin ◽  
Quin H. Christensen ◽  
María C. Mansilla ◽  
John E. Cronan ◽  
Diego de Mendoza
Keyword(s):  
Bacillus Subtilis ◽  
Lipoic Acid ◽  
Acid Biosynthesis

scholarly journals Structural basis for bacterial ribosome quality control

2020 ◽  
Author(s):  
Caillan Crowe-McAuliffe ◽  
Hiraku Takada ◽  
Victoriia Murina ◽  
Christine Polte ◽  
Sergo Kasvandik ◽  
...  
Keyword(s):  
Quality Control ◽  
Bacillus Subtilis ◽  
Single Particle ◽  
Ex Vivo ◽  
Large Subunit ◽  
Small Subunit ◽  
Structural Basis ◽  
Bacterial Ribosome

SummaryIn all branches of life, stalled translation intermediates are recognized and processed by ribosome-associated quality-control (RQC) pathways. RQC begins with splitting of stalled ribosomes, leaving an unfinished polypeptide still attached to the large subunit. Ancient and conserved NEMF family RQC proteins target these incomplete proteins for degradation by the addition of C-terminal ‘tails.’ How such tailing can occur without the regular suite of translational components is, however, unclear. Using ex vivo single-particle cryo-EM, we show that C-terminal tailing in Bacillus subtilis is mediated by NEMF protein RqcH in concert with YabO, a protein homologous to, yet distinct from, Hsp15. Our structures reveal how these factors mediate tRNA movement across the ribosomal 50S subunit to synthesize polypeptides in the absence of mRNA or the small subunit.

scholarly journals Precise Deletion of tagD and Controlled Depletion of Its Product, Glycerol 3-Phosphate Cytidylyltransferase, Leads to Irregular Morphology and Lysis of Bacillus subtilisGrown at Physiological Temperature

2001 ◽  
Vol 183 (22) ◽  
pp. 6688-6693 ◽  
Author(s):  
Amit P. Bhavsar ◽  
Terry J. Beveridge ◽  
Eric D. Brown
Keyword(s):  
Bacillus Subtilis ◽  
Gene Product ◽  
Genetic Background ◽  
Expression System ◽  
Teichoic Acid ◽  
Structural Defects ◽  
Acid Biosynthesis ◽  
Physiological Temperature ◽  
Conditional Expression ◽  
Phenotypic Rescue

ABSTRACT Using a previously reported conditional expression system for use in Bacillus subtilis (A. P. Bhavsar, X. Zhao, and E. D. Brown, Appl. Environ. Microbiol. 67:403–410, 2001), we report the first precise deletion of a teichoic acid biosynthesis (tag) gene, tagD,in B. subtilis. This teichoic acid mutant showed a lethal phenotype when characterized at a physiological temperature and in a defined genetic background. This tagD mutant was subject to full phenotypic rescue upon expression of the complementing copy oftagD. Depletion of the tagD gene product (glycerol 3-phosphate cytidylyltransferase) via modulated expression of tagD from the amyE locus revealed structural defects centered on shape, septation, and division. Thickening of the wall and ultimately lysis followed these events.

scholarly journals A Bifunctional Enzyme Catalyzes the First Two Steps inN-Acetylneuraminic Acid Biosynthesis of Rat Liver

1997 ◽  
Vol 272 (39) ◽  
pp. 24319-24324 ◽  
Author(s):  
Roger Stäsche ◽  
Stephan Hinderlich ◽  
Christoph Weise ◽  
Karin Effertz ◽  
Lothar Lucka ◽  
...  
Keyword(s):  
Rat Liver ◽  
Bifunctional Enzyme ◽  
Acid Biosynthesis ◽  
Acetylneuraminic Acid

scholarly journals Insufficient Expression of the ilv-leu Operon Encoding Enzymes of Branched-Chain Amino Acid Biosynthesis Limits Growth of a Bacillus subtilis ccpA Mutant

2002 ◽  
Vol 184 (18) ◽  
pp. 5174-5178 ◽  
Author(s):  
Holger Ludwig ◽  
Christoph Meinken ◽  
Anastasija Matin ◽  
Jörg Stülke
Keyword(s):  
Bacillus Subtilis ◽  
Amino Acid ◽  
Optimal Growth ◽  
Growth Defect ◽  
Acid Biosynthesis ◽  
Branched Chain Amino Acid ◽  
Mutant Strains ◽  
Regulatory Link ◽  
Branched Chain ◽  
Control Of Expression

ABSTRACT Bacillus subtilis ccpA mutant strains exhibit two distinct phenotypes: they are defective in catabolite repression, and their growth on minimal media is strongly impaired. This growth defect is largely due to a lack of expression of the gltAB operon. However, growth is impaired even in the presence of glutamate. Here, we demonstrate that the ccpA mutant strain needs methionine and the branched-chain amino acids for optimal growth. The control of expression of the ilv-leu operon by CcpA provides a novel regulatory link between carbon and amino acid metabolism.

scholarly journals Regulation of colominic acid biosynthesis by temperature: Role of cytidine 5′-monophosphateN-acetylneuraminic acid synthetase

FEBS Letters ◽  
1989 ◽  
Vol 250 (2) ◽  
pp. 429-432 ◽  
Author(s):  
Camino González-Clemente ◽  
José M. Luengo ◽  
Leandro B. Rodríguez-Aparicio ◽  
Angel Reglero
Keyword(s):  
Acid Biosynthesis ◽  
Acetylneuraminic Acid ◽  
Colominic Acid

Pathway Engineering of Bacillus subtilis for Enhanced N ‐Acetylneuraminic Acid Production via Whole‐Cell Biocatalysis

2019 ◽  
Vol 14 (7) ◽  
pp. 1800682 ◽  
Author(s):  
Lin Zhao ◽  
Rongzhen Tian ◽  
Qingyang Shen ◽  
Yanfeng Liu ◽  
Long Liu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Acid Production ◽  
Pathway Engineering ◽  
Whole Cell ◽  
Acetylneuraminic Acid ◽  
Whole Cell Biocatalysis
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