Identification and Characterization of the Mitochondrial Replication Origin for Stable and Episomal Expression in Yarrowia lipolytica

2021 ◽  
Author(s):  
Zhiyong Cui ◽  
Huihui Zheng ◽  
Zhennan Jiang ◽  
Zhaoxuan Wang ◽  
Jin Hou ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Replication Origin ◽  
Mitochondrial Replication ◽  
Identification And Characterization

Identification and characterization of two α-1,6-mannosyltransferases, Anl1p and Och1p, in the yeast Yarrowia lipolytica

Microbiology ◽  
2004 ◽  
Vol 150 (7) ◽  
pp. 2185-2195 ◽  
Author(s):  
Stéphanie Barnay-Verdier ◽  
Anita Boisramé ◽  
Jean-Marie Beckerich
Keyword(s):  
Yarrowia Lipolytica ◽  
Phenotypic Analysis ◽  
Calcofluor White ◽  
Direct Role ◽  
Increased Sensitivity ◽  
Glycosylation Pathway ◽  
Identification And Characterization ◽  
Glycosylation Defect

In this study, the identification and characterization of the Yarrowia lipolytica homologues of Saccharomyces cerevisiae α-1,6-mannosyltransferases Anp1p and Och1p, designated YlAnl1p and YlOch1p, are described. In order to confirm the function of the Y. lipolytica proteins, including the previously isolated YlMnn9p, in the N-glycosylation pathway, a phenotypic analysis of the disrupted strains ΔYlmnn9, ΔYlanl1, ΔYloch1, ΔYlanl1ΔYlmnn9 and ΔYlmnn9ΔYloch1 was performed. Disruption of the YlMNN9, YlANL1 and YlOCH1 genes caused an increased sensitivity to SDS, compatible with a glycosylation defect, and to Calcofluor White, characteristic of cell-wall defects. Moreover, Western-blot analysis of a heterologous glycosylated protein confirmed a direct role of YlMnn9p and YlAnl1p in the N-glycosylation process. These mutant strains, ΔYlmnn9, ΔYlanl1, ΔYloch1, ΔYlanl1ΔYlmnn9 and ΔYlmnn9ΔYloch1 may thus be used to establish a model for the Y. lipolytica N-linked glycosylation pathway.

Identification and characterization of EYD1, encoding an erythritol dehydrogenase in Yarrowia lipolytica and its application to bioconvert erythritol into erythrulose

2018 ◽  
Vol 247 ◽  
pp. 963-969 ◽  
Author(s):  
Frédéric Carly ◽  
Sébastien Steels ◽  
Samuel Telek ◽  
Marie Vandermies ◽  
Jean-Marc Nicaud ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Identification And Characterization

scholarly journals Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags

2018 ◽  
Vol 102 (12) ◽  
pp. 5221-5233 ◽  
Author(s):  
Ewelina Celińska ◽  
Monika Borkowska ◽  
Wojciech Białas ◽  
Paulina Korpys ◽  
Jean-Marc Nicaud
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Secretion ◽  
Signal Peptides ◽  
Identification And Characterization

scholarly journals Erratum to: Identification and characterization of EYK1, a key gene for erythritol catabolism in Yarrowia lipolytica

2017 ◽  
Vol 101 (16) ◽  
pp. 6573-6573 ◽  
Author(s):  
F. Carly ◽  
H. Gamboa-Melendez ◽  
M. Vandermies ◽  
C. Damblon ◽  
J. M. Nicaud ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Identification And Characterization

Identification and characterization of a complex chromosomal replication origin inSchizosaccharomyces pombe

Chromosoma ◽  
1992 ◽  
Vol 102 (S1) ◽  
pp. S7-S16 ◽  
Author(s):  
Jiguang Zhu ◽  
Christine Brun ◽  
Hisanori Kurooka ◽  
Mitsuhiro Yanagida ◽  
Joel A. Huberman
Keyword(s):  
Replication Origin ◽  
Chromosomal Replication ◽  
Identification And Characterization

Identification and characterization of EYK1, a key gene for erythritol catabolism in Yarrowia lipolytica

2017 ◽  
Vol 101 (17) ◽  
pp. 6587-6596 ◽  
Author(s):  
F. Carly ◽  
H. Gamboa-Melendez ◽  
M. Vandermies ◽  
C. Damblon ◽  
J. M. Nicaud ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Identification And Characterization
Sign in / Sign up

Export Citation Format

Share Document