Elucidating the Membrane Dynamics and Encapsulation Mechanism of Large DNA Molecules Under Molecular Crowding Conditions Using Giant Unilamellar Vesicles

2020 ◽  
Vol 9 (10) ◽  
pp. 2819-2827
Author(s):  
Mamiko Tsugane ◽  
Hiroaki Suzuki
Keyword(s):  
Membrane Dynamics ◽  
Molecular Crowding ◽  
Dna Molecules ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

scholarly journals DNA translocation to giant unilamellar vesicles during electroporation is independent of DNA size

Soft Matter ◽  
2019 ◽  
Vol 15 (45) ◽  
pp. 9187-9194
Author(s):  
Shaurya Sachdev ◽  
Aswin Muralidharan ◽  
Dipendra K. Choudhary ◽  
Dayinta L. Perrier ◽  
Lea Rems ◽  
...  
Keyword(s):  
Electrophoretic Mobility ◽  
Dna Delivery ◽  
Dna Molecules ◽  
Dna Translocation ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Dna Size

DNA delivery into GUVs during electroporation is governed by bulk electrophoretic mobility implying a mechanism in which DNA molecules enter in their coiled conformation, as opposed to stochastic threading, through electro-pores.

scholarly journals Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles

2019 ◽  
Vol 55 (62) ◽  
pp. 9084-9087 ◽  
Author(s):  
Yusuke Sato ◽  
Ken Komiya ◽  
Ibuki Kawamata ◽  
Satoshi Murata ◽  
Shin-ichiro M. Nomura
Keyword(s):  
Isothermal Amplification ◽  
Dna Molecules ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Artificial Dna

An artificial DNA circuit in giant unilamellar vesicles allows isothermal amplification of DNA by over 5000-fold within several hours.

scholarly journals Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 634
Author(s):  
Ylenia Miele ◽  
Gábor Holló ◽  
István Lagzi ◽  
Federico Rossi
Keyword(s):  
Phospholipid Fatty Acid ◽  
Enzymatic Reaction ◽  
Stimuli Responsive ◽  
Membrane Composition ◽  
Giant Unilamellar Vesicles ◽  
Physical Processes ◽  
Unilamellar Vesicles ◽  
Division Process ◽  
The Origin Of Life ◽  
Area Difference

The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles.

Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass

Langmuir ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 1082-1088
Author(s):  
Chiho Kataoka-Hamai ◽  
Kohsaku Kawakami
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

scholarly journals Production of giant unilamellar vesicles and encapsulation of lyotropic nematic liquid crystals

Soft Matter ◽  
2021 ◽  
Author(s):  
Peng Bao ◽  
Daniel A. Paterson ◽  
Sally A. Peyman ◽  
J. Cliff Jones ◽  
Jonathan A. T. Sandoe ◽  
...  
Keyword(s):  
Liquid Crystals ◽  
Nematic Liquid Crystals ◽  
Double Emulsion ◽  
Lyotropic Liquid Crystals ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Emulsion Droplets ◽  
Double Emulsion Droplets

We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets and encapsulation of nematic lyotropic liquid crystals (LNLCs).

scholarly journals Inter- and Intra-Plane Interactions Control the Existence of Macrodomains in Asymmetric Giant Unilamellar Vesicles

2021 ◽  
Vol 120 (3) ◽  
pp. 147a
Author(s):  
Thais A. Enoki ◽  
Haden L. Scott ◽  
Gerald W. Feigenson ◽  
Frederick A. Heberle
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles
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