Component Optimization of Neomycin Biosynthesis via the Reconstitution of a Combinatorial Mini-Gene-Cluster in Streptomyces fradiae

2020 ◽  
Vol 9 (9) ◽  
pp. 2493-2501
Author(s):  
Jiazhen Zheng ◽  
Yue Li ◽  
Hanye Guan ◽  
Junyue Li ◽  
Dong Li ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Streptomyces Fradiae

The lipopeptide antibiotic A54145 biosynthetic gene cluster from Streptomyces fradiae

2005 ◽  
Vol 33 (2) ◽  
pp. 129-140 ◽  
Author(s):  
Vivian Miao ◽  
Renee Brost ◽  
Joanne Chapple ◽  
Kevin She ◽  
Marie-François Coëffet-Le Gal ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Streptomyces Fradiae ◽  
Lipopeptide Antibiotic

Characterization of RbmD (Glycosyltransferase in Ribostamycin Gene Cluster) through neomycin production reconstituted from the engineered Streptomyces fradiae BS1

2008 ◽  
Vol 27 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Keshav Kumar Nepal ◽  
Tae-Jin Oh ◽  
Bimala Subba ◽  
Jin Cheol Yoo ◽  
Jae Kyung Sohng
Keyword(s):  
Gene Cluster ◽  
Streptomyces Fradiae

scholarly journals Development of a Genetic System for Combinatorial Biosynthesis of Lipopeptides in Streptomyces fradiae and Heterologous Expression of the A54145 Biosynthesis Gene Cluster

2010 ◽  
Vol 76 (20) ◽  
pp. 6877-6887 ◽  
Author(s):  
Dylan C. Alexander ◽  
Jessica Rock ◽  
Xiaowei He ◽  
Paul Brian ◽  
Vivian Miao ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Glutamic Acid ◽  
Gene Cluster ◽  
Genetic System ◽  
Combinatorial Biosynthesis ◽  
Streptomyces Fradiae ◽  
Bac Clone ◽  
Biosynthesis Gene Cluster ◽  
Streptomyces Ambofaciens ◽  
Biosynthesis Gene

ABSTRACT A54145 factors are calcium-dependent lipopeptide antibiotics produced by Streptomyces fradiae NRRL 18160. A54145 is structurally related to the clinically important daptomycin, and as such may be a useful scaffold for the development of a novel lipopeptide antibiotic. We developed methods to genetically manipulate S. fradiae by deletion mutagenesis and conjugal transfer of plasmids from Escherichia coli. Cloning the complete pathway on a bacterial artificial chromosome (BAC) vector and the construction of ectopic trans-complementation with plasmids utilizing the φC31 or φBT1 site-specific integration system allowed manipulation of A54145 biosynthesis. The BAC clone pDA2002 was shown to harbor the complete A54145 biosynthesis gene cluster by heterologous expression in Streptomyces ambofaciens and Streptomyces roseosporus strains in yields of >100 mg/liter. S. fradiae mutants defective in LptI methyltransferase function were constructed, and they produced only A54145 factors containing glutamic acid (Glu12), at the expense of factors containing 3-methyl-glutamic acid (3mGlu12). This provided a practical route to produce high levels of pure Glu12-containing lipopeptides. A suite of mutant strains and plasmids was created for combinatorial biosynthesis efforts focused on modifying the A54145 peptide backbone to generate a compound with daptomycin antibacterial activity and activity in Streptococcus pneumoniae pulmonary infections.

The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine

2005 ◽  
Vol 3 (8) ◽  
pp. 1410 ◽  
Author(s):  
Fanglu Huang ◽  
Stephen F. Haydock ◽  
Tatiana Mironenko ◽  
Dieter Spiteller ◽  
Yanyan Li ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Streptomyces Fradiae
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