Exploring Catalysis Specificity of Phytoene Dehydrogenase CrtI in Carotenoid Synthesis

2020 ◽  
Vol 9 (7) ◽  
pp. 1753-1762
Author(s):  
Nan Liang ◽  
Chen Chen ◽  
Ying Wang ◽  
Ming-Zhu Ding ◽  
Ming-Dong Yao ◽  
...  
Keyword(s):  
Phytoene Dehydrogenase ◽  
Carotenoid Synthesis

scholarly journals RegA Control of Bacteriochlorophyll and Carotenoid Synthesis in Rhodobacter capsulatus

2007 ◽  
Vol 189 (21) ◽  
pp. 7765-7773 ◽  
Author(s):  
Jonathan Willett ◽  
James L. Smart ◽  
Carl E. Bauer
Keyword(s):  
Rhodobacter Capsulatus ◽  
Protoporphyrin Ix ◽  
Carotenoid Biosynthesis ◽  
Divergent Promoters ◽  
Anaerobic Induction ◽  
Single Region ◽  
Phytoene Dehydrogenase ◽  
Carotenoid Synthesis

ABSTRACT We provide in vivo genetic and in vitro biochemical evidence that RegA directly regulates bacteriochlorophyll and carotenoid biosynthesis in Rhodobacter capsulatus. β-Galactosidase expression assays with a RegA-disrupted strain containing reporter plasmids for Mg-protoporphyrin IX monomethyl ester oxidative cyclase (bchE), Mg-protoporphyrin IX chelatase (bchD), and phytoene dehydrogenase (crtI) demonstrate RegA is responsible for fourfold anaerobic induction of bchE, threefold induction of bchD, and twofold induction of crtI. Promoter mapping studies, coupled with DNase I protection assays, map the region of RegA binding to three sites in the bchE promoter region. Similar studies at the crtA and crtI promoters indicate that RegA binds to a single region equidistant from these divergent promoters. These results demonstrate that RegA is directly responsible for anaerobic induction of bacteriochlorophyll biosynthesis genes bchE, bchD, bchJ, bchI, bchG, and bchP and carotenoid biosynthesis genes crtI, crtB, and crtA.

scholarly journals Combined Production of Astaxanthin and β-Carotene in a New Strain of the Microalga Bracteacoccus aggregatus BM5/15 (IPPAS C-2045) Cultivated in Photobioreactor

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 643
Author(s):  
Konstantin Chekanov ◽  
Daniil Litvinov ◽  
Tatiana Fedorenko ◽  
Olga Chivkunova ◽  
Elena Lobakova
Keyword(s):  
Bubble Column ◽  
Biomass Accumulation ◽  
Natural Antioxidants ◽  
Gas Chromatography Mass Spectrometry ◽  
Stage Scheme ◽  
Green Microalga ◽  
Animal Feeding ◽  
Carotenoid Synthesis ◽  
Bubble Column Photobioreactor ◽  
Β Carotene

Carotenoids astaxanthin and β-carotene are widely used natural antioxidants. They are key components of functional food, cosmetics, drugs and animal feeding. They hold leader positions on the world carotenoid market. In current work, we characterize the new strain of the green microalga Bracteacoccus aggregatus BM5/15 and propose the method of its culturing in a bubble-column photobioreactor for simultaneous production of astaxanthin and β-carotene. Culture was monitored by light microscopy and pigment kinetics. Fatty acid profile was evaluated by tandem gas-chromatography–mass spectrometry. Pigments were obtained by the classical two-stage scheme of autotrophic cultivation. At the first, vegetative, stage biomass accumulation occurred. Maximum specific growth rate and culture productivity at this stage were 100–200 mg∙L−1∙day−1, and 0.33 day−1, respectively. At the second, inductive, stage carotenoid synthesis was promoted. Maximal carotenoid fraction in the biomass was 2.2–2.4%. Based on chromatography data, astaxanthin and β-carotene constituted 48 and 13% of total carotenoid mass, respectively. Possible pathways of astaxanthin synthesis are proposed based on carotenoid composition. Collectively, a new strain B. aggregatus BM5/15 is a potential biotechnological source of two natural antioxidants, astaxanthin and β-carotene. The results give the rise for further works on optimization of B. aggregatus cultivation on an industrial scale.

scholarly journals Functional Lycopene Cyclase (CruA) in Cyanobacterium, Arthrospira platensis NIES-39, and its Role in Carotenoid Synthesis

2017 ◽  
Vol 58 (4) ◽  
pp. 831-838 ◽  
Author(s):  
Kenjiro Sugiyama ◽  
Masashi Ebisawa ◽  
Masaharu Yamada ◽  
Yoshiki Nagashima ◽  
Hideyuki Suzuki ◽  
...  
Keyword(s):  
Arthrospira Platensis ◽  
Lycopene Cyclase ◽  
Carotenoid Synthesis

scholarly journals Potential of macrophyte for removing arsenic from aqueous solution

2012 ◽  
Vol 30 (4) ◽  
pp. 683-696 ◽  
Author(s):  
F.P. Guimaraes ◽  
R. Aguiar ◽  
J.A. Oliveira ◽  
J.A.A. Silva ◽  
D. Karam
Keyword(s):  
Aquatic Macrophytes ◽  
Leaf Size ◽  
Mass Gain ◽  
Dry Mass ◽  
Phosphate Deficiency ◽  
Lemna Gibba ◽  
High Concentration ◽  
Carotenoid Synthesis ◽  
High Concentrations ◽  
Selection Of

The potential of three aquatic macrophytes, Azoll caroliniana, Salvinia minima and Lemna gibba, was evaluated in this work aimed at selection of plants to be used in remediation of environments contaminated by arsenic (As). The experiments were carried out in a greenhouse during six days in pots containing Hoagland solution (¼ ionic strength) at As concentrations of 0.5; 2.5 and 5.0 mg L-1. The three species showed greater As accumulation as the concentration of the metalloid in solution increased. However, a reduction was detected in fresh and dry mass gain when the plants were exposed to high As concentrations. The macrophytes showed differences in efficiency of removal of As in solution. A. caroliniana, S. minima and L. gibba accumulated, on average, 0.130; 0.200; and 1.397 mg mDM-1, respectively, when exposed to 5.0 mg L-1 of As. The macrophytes absorbed a greater quantity of As in solution with low phosphate content. The greater As concentration in L. gibba tissues lowered the chlorophyll and carotenoid contents as shown by the high chlorosis incidence. Lemna gibba also exhibited a decrease in leaf size, with the total chlorophyll and carotenoid synthesis not being affected by As in A. caroliniana. This species exhibited purplish leaves with high concentration of anthocyanin, whose presence suggested association to phosphate deficiency. Marginal necrosis occurred on S. minima floating leaves, with the released daughter-plants not showing any visual symptoms during the treatment. The percentage of As removed from the solution decreased when the plants were exposed to high concentrations of the pollutant. Among the three species studied, only L. gibba could be considered an As hyper-accumulator. The use of this plant species for remediation of aquatic environments was shown to be limited and requires further investigation.

ChemInform Abstract: Carotenoid Synthesis: A Progress Report

ChemInform ◽  
2010 ◽  
Vol 29 (6) ◽  
pp. no-no
Author(s):  
H. PFANDER ◽  
B. TRABER ◽  
M. LANZ
Keyword(s):  
Progress Report ◽  
Carotenoid Synthesis

scholarly journals Suppression of carotenoid synthesis in transgenic Arabidopsis cultured cells over-expressing the AHL29/SOB3 gene

2008 ◽  
Vol 25 (6) ◽  
pp. 573-577
Author(s):  
Ryosuke Sano ◽  
Hideyuki Suzuki ◽  
Yoichi Ogawa ◽  
Tomoko Dansako ◽  
Nozomu Sakurai ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Transgenic Arabidopsis ◽  
Carotenoid Synthesis
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