Purification of an Intact Human Protein Overexpressed from Its Endogenous Locus via Direct Genome Engineering

Jihyeon Yu; Eunju Cho; Yeon-Gil Choi; You Kyeong Jeong; Yongwoo Na; Jong-Seo Kim; Sung-Rae Cho; Jae-Sung Woo; Sangsu Bae

doi:10.1021/acssynbio.0c00090

Purification of an Intact Human Protein Overexpressed from Its Endogenous Locus via Direct Genome Engineering

ACS Synthetic Biology ◽

10.1021/acssynbio.0c00090 ◽

2020 ◽

Vol 9 (7) ◽

pp. 1591-1598 ◽

Cited By ~ 1

Author(s):

Jihyeon Yu ◽

Eunju Cho ◽

Yeon-Gil Choi ◽

You Kyeong Jeong ◽

Yongwoo Na ◽

...

Keyword(s):

Genome Engineering ◽

Human Protein ◽

Endogenous Locus

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Chromatographic purification and properties of a therapeutic human protein C concentrate

Journal of Chromatography B ◽

10.1016/s0021-9673(02)02002-2 ◽

2003 ◽

Author(s):

M Radosevich

Keyword(s):

Protein C ◽

Human Protein ◽

Chromatographic Purification ◽

Purification And Properties ◽

Protein C Concentrate

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A New Functional Assay for Human Protein S Activity Using Activated Factor Vas Substrate

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647133 ◽

1989 ◽

Vol 62 (04) ◽

pp. 1144-1145 ◽

Cited By ~ 49

Author(s):

Martine Wolf ◽

Catherine Boyer-Neumann ◽

Jean-Luc Martinoli ◽

Catherine Leroy-Matheron ◽

Amiral Jean ◽

...

Keyword(s):

Protein S ◽

Human Protein ◽

Functional Assay ◽

Protein S Activity

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Faculty Opinions recommendation of Widespread purifying selection at polymorphic sites in human protein-coding loci.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1016701.201233 ◽

2003 ◽

Cited By ~ 1

Author(s):

Thomas Mitchell-Olds

Keyword(s):

Purifying Selection ◽

Human Protein ◽

Protein Coding

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Faculty Opinions recommendation of Distribution of SR protein exonic splicing enhancer motifs in human protein-coding genes.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1027960.335073 ◽

2005 ◽

Author(s):

Lawrence Chasin

Keyword(s):

Human Protein ◽

Exonic Splicing Enhancer ◽

Sr Protein ◽

Protein Coding ◽

Protein Coding Genes ◽

Splicing Enhancer

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Faculty Opinions recommendation of Interaction between intrinsically disordered proteins frequently occurs in a human protein-protein interaction network.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1164197.624888 ◽

2009 ◽

Author(s):

Vladimir Uversky

Keyword(s):

Protein Interaction ◽

Protein Interaction Network ◽

Intrinsically Disordered Proteins ◽

Interaction Network ◽

Human Protein ◽

Disordered Proteins ◽

Protein Protein Interaction ◽

Intrinsically Disordered ◽

Protein Protein Interaction Network

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Faculty Opinions recommendation of Profiling the human protein-DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1167956.648054 ◽

2009 ◽

Author(s):

Trey Ideker ◽

Sourav Bandyopadhyay

Keyword(s):

Transcriptional Repressor ◽

Human Protein ◽

Interferon Signaling

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Faculty Opinions recommendation of A systematic survey of loss-of-function variants in human protein-coding genes.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13923985.15512056 ◽

2012 ◽

Author(s):

Isaac Kohane

Keyword(s):

Human Protein ◽

Loss Of Function ◽

Protein Coding ◽

Systematic Survey ◽

Protein Coding Genes

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Faculty Opinions recommendation of Systematic analysis of human protein complexes identifies chromosome segregation proteins.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.5558956.5527068 ◽

2010 ◽

Author(s):

Ruedi Aebersold ◽

Lars Malmstroem

Keyword(s):

Chromosome Segregation ◽

Protein Complexes ◽

Human Protein ◽

Systematic Analysis

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Faculty Opinions recommendation of Global landscape of HIV-human protein complexes.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717965045.793466207 ◽

2012 ◽

Author(s):

Ruedi Aebersold ◽

Lars Malmstroem

Keyword(s):

Protein Complexes ◽

Human Protein

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Faculty Opinions recommendation of One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718007692.793477327 ◽

2013 ◽

Author(s):

Jakub Tolar ◽

Michael Vanden Oever

Keyword(s):

Genome Engineering ◽

Multiple Genes ◽

One Step ◽

Step Generation

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