Near-Infrared-Emitting Probes for Detection of Nanomolar Hydrazine in a Complete Aqueous Medium with Real-Time Application in Bioimaging and Vapor-Phase Hydrazine Detection

2020 ◽  
Vol 8 (11) ◽  
pp. 4457-4463 ◽  
Author(s):  
Natarajan Vijay ◽  
Sivan Velmathi
Keyword(s):  
Aqueous Medium ◽  
Real Time ◽  
Vapor Phase ◽  
Near Infrared ◽  
Real Time Application

scholarly journals Evaluation of Optimal Vibrotactile Feedback for Force-Controlled Upper Limb Myoelectric Prostheses

Sensors ◽  
2019 ◽  
Vol 19 (23) ◽  
pp. 5209 ◽  
Author(s):  
Andrea Gonzalez-Rodriguez ◽  
Jose L. Ramon ◽  
Vicente Morell ◽  
Gabriel J. Garcia ◽  
Jorge Pomares ◽  
...  
Keyword(s):  
Real Time ◽  
Upper Limb ◽  
Closed Loop ◽  
Fine Tuning ◽  
Vibrotactile Feedback ◽  
Loop Control ◽  
Myoelectric Prostheses ◽  
Amplitude Level ◽  
Real Time Application ◽  
Selection Of

The main goal of this study is to evaluate how to optimally select the best vibrotactile pattern to be used in a closed loop control of upper limb myoelectric prostheses as a feedback of the exerted force. To that end, we assessed both the selection of actuation patterns and the effects of the selection of frequency and amplitude parameters to discriminate between different feedback levels. A single vibrotactile actuator has been used to deliver the vibrations to subjects participating in the experiments. The results show no difference between pattern shapes in terms of feedback perception. Similarly, changes in amplitude level do not reflect significant improvement compared to changes in frequency. However, decreasing the number of feedback levels increases the accuracy of feedback perception and subject-specific variations are high for particular participants, showing that a fine-tuning of the parameters is necessary in a real-time application to upper limb prosthetics. In future works, the effects of training, location, and number of actuators will be assessed. This optimized selection will be tested in a real-time proportional myocontrol of a prosthetic hand.

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

scholarly journals Research on Real-time Application of Ubiquitous Power Internet of Things Information Based on Smart Sensor Technology

2021 ◽  
Vol 714 (4) ◽  
pp. 042046
Author(s):  
Jiangping Nan ◽  
Yajuan Jia ◽  
Xuezhen Dai ◽  
Yinglu Liu ◽  
Xiaowen Ren ◽  
...  
Keyword(s):  
Internet Of Things ◽  
Real Time ◽  
Sensor Technology ◽  
Smart Sensor ◽  
Real Time Application

scholarly journals A hybrid upconversion nanoprobe for ratiometric detection of aliphatic biogenic amines in aqueous medium

2021 ◽  
Author(s):  
Shilpi Jaiswal ◽  
Subhankar Kundu ◽  
Sujoy Bandyopadhyay ◽  
Abhijit Patra
Keyword(s):  
Aqueous Medium ◽  
Biogenic Amines ◽  
Real Time ◽  
Inorganic Hybrid ◽  
The Real ◽  
Ratiometric Detection ◽  
Real Time Detection

An organic–inorganic hybrid upconversion nanoprobe was developed for the real-time detection of aliphatic biogenic amines in an aqueous medium, adulterated milk, and rotten fish.

Real-time tracking lysosomal pH changes under heatstroke and redox stress with a novel near-infrared emissive probe

Talanta ◽  
2021 ◽  
Vol 228 ◽  
pp. 122184
Author(s):  
Qingfeng Xia ◽  
Shumin Feng ◽  
Jiaxin Hong ◽  
Guoqiang Feng
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Redox Stress ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Real Time Tracking ◽  
Emissive Probe
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