Field-Grown Transgenic Hybrid Poplar with Modified Lignin Biosynthesis to Improve Enzymatic Saccharification Efficiency

2017 ◽  
Vol 5 (3) ◽  
pp. 2407-2414 ◽  
Author(s):  
Zhouyang Xiang ◽  
Suman Kumar Sen ◽  
Douyong Min ◽  
Dhanalekshmi Savithri ◽  
Fachuang Lu ◽  
...  
Keyword(s):  
Hybrid Poplar ◽  
Enzymatic Saccharification ◽  
Lignin Biosynthesis ◽  
Saccharification Efficiency ◽  
Modified Lignin

scholarly journals CRISPR-Knockout of CSE Gene Improves Saccharification Efficiency by Reducing Lignin Content in Hybrid Poplar

2021 ◽  
Vol 22 (18) ◽  
pp. 9750
Author(s):  
Hyun-A Jang ◽  
Eun-Kyung Bae ◽  
Min-Ha Kim ◽  
Su-Jin Park ◽  
Na-Young Choi ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Lignin Content ◽  
Hybrid Poplar ◽  
Lignin Biosynthesis ◽  
Biofuel Production ◽  
Guide Rnas ◽  
Hybrid Poplars ◽  
Four Seasons ◽  
Promising Target ◽  
Saccharification Efficiency

Caffeoyl shikimate esterase (CSE) has been shown to play an important role in lignin biosynthesis in plants and is, therefore, a promising target for generating improved lignocellulosic biomass crops for sustainable biofuel production. Populus spp. has two CSE genes (CSE1 and CSE2) and, thus, the hybrid poplar (Populus alba × P. glandulosa) investigated in this study has four CSE genes. Here, we present transgenic hybrid poplars with knockouts of each CSE gene achieved by CRISPR/Cas9. To knockout the CSE genes of the hybrid poplar, we designed three single guide RNAs (sg1–sg3), and produced three different transgenic poplars with either CSE1 (CSE1-sg2), CSE2 (CSE2-sg3), or both genes (CSE1/2-sg1) mutated. CSE1-sg2 and CSE2-sg3 poplars showed up to 29.1% reduction in lignin deposition with irregularly shaped xylem vessels. However, CSE1-sg2 and CSE2-sg3 poplars were morphologically indistinguishable from WT and showed no significant differences in growth in a long-term living modified organism (LMO) field-test covering four seasons. Gene expression analysis revealed that many lignin biosynthetic genes were downregulated in CSE1-sg2 and CSE2-sg3 poplars. Indeed, the CSE1-sg2 and CSE2-sg3 poplars had up to 25% higher saccharification efficiency than the WT control. Our results demonstrate that precise editing of CSE by CRISPR/Cas9 technology can improve lignocellulosic biomass without a growth penalty.

scholarly journals Characterization of lignocellulose of Erianthus arundinaceus in relation to enzymatic saccharification efficiency

2013 ◽  
Vol 30 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Masaomi Yamamura ◽  
Soichiro Noda ◽  
Takefumi Hattori ◽  
Amiu Shino ◽  
Jun Kikuchi ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Erianthus Arundinaceus ◽  
Saccharification Efficiency

Recycling of Black Liquor Generated from Cellulosic Ethanol Process for Plant Growth

2020 ◽  
Vol 14 (4) ◽  
pp. 511-516
Author(s):  
Changzhong Song ◽  
Bowen Zhang ◽  
Wen Wang ◽  
Xuesong Tan ◽  
Zahoor ◽  
...  
Keyword(s):  
Plant Growth ◽  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Black Liquor ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Alkaline Pretreatment ◽  
Solid Loading ◽  
Solid Ratio ◽  
Saccharification Efficiency

The alkaline pretreatment has the advantages of low energy input and atmospheric pressure to highly enhance the conversion of lignocellulose to ethanol. However, the black liquor from the process would pollute the environment, which hinders its industrial application. This study selected the potassium hydroxide (KOH) as the alkaline reagent for lignocellulosic pretreatment and investigated the feasibility of recycling the black liquor (BL) as molecular bio-activator for plant growth. After optimization of KOH pretreatment, the enzymatic saccharification efficiency of rice straw achieved to 86.6% under the optimum condition of 2% KOH, 15:1 of liquid–solid ratio, 70 °C for 1 h. The ethanol production and conversion ratio was 32.24 g/L and 53.0% respectively at 20% solid loading. The tobacco cultured in the nutrient solution with BL was more luxuriant than that without BL, of which the dry weight of plant increased 367% and the leaf area increment of tobacco was about 2∼4 times than the control after 30 days. Thus this study provided a promising way to accelerate the industrialization of alkaline pretreatment for cellulosic ethanol production.

scholarly journals Chemical responses to modified lignin composition in tension wood of hybrid poplar (Populus tremula x Populus alba)

2013 ◽  
Vol 33 (4) ◽  
pp. 365-373 ◽  
Author(s):  
J. M. Al-Haddad ◽  
K.-Y. Kang ◽  
S. D. Mansfield ◽  
F. W. Telewski
Keyword(s):  
Tension Wood ◽  
Hybrid Poplar ◽  
Populus Tremula ◽  
Populus Alba ◽  
Lignin Composition ◽  
Modified Lignin

scholarly journals Ectomycorrhizal Colonization and Diversity in Relation to Tree Biomass and Nutrition in a Plantation of Transgenic Poplars with Modified Lignin Biosynthesis

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e59207 ◽  
Author(s):  
Lara Danielsen ◽  
Gertrud Lohaus ◽  
Anke Sirrenberg ◽  
Petr Karlovsky ◽  
Catherine Bastien ◽  
...  
Keyword(s):  
Lignin Biosynthesis ◽  
Tree Biomass ◽  
Ectomycorrhizal Colonization ◽  
Transgenic Poplars ◽  
Modified Lignin
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