scholarly journals OLIVe: A Genetically Encoded Fluorescent Biosensor for Quantitative Imaging of Branched-Chain Amino Acid Levels inside Single Living Cells

ACS Sensors ◽  
2019 ◽  
Vol 4 (12) ◽  
pp. 3333-3342 ◽  
Author(s):  
Tomoki Yoshida ◽  
Hitomi Nakajima ◽  
Sena Takahashi ◽  
Akira Kakizuka ◽  
Hiromi Imamura
Keyword(s):  
Amino Acid ◽  
Quantitative Imaging ◽  
Living Cells ◽  
Fluorescent Biosensor ◽  
Branched Chain Amino Acid ◽  
Amino Acid Levels ◽  
Branched Chain ◽  
Single Living Cells ◽  
Single Living

scholarly journals Branched-Chain Amino Acid Levels Are Related with Surrogates of Disturbed Lipid Metabolism among Older Men

2016 ◽  
Vol 3 ◽  
Author(s):  
Urho M. Kujala ◽  
Markku Peltonen ◽  
Merja K. Laine ◽  
Jaakko Kaprio ◽  
Olli J. Heinonen ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Amino Acid ◽  
Older Men ◽  
Branched Chain Amino Acid ◽  
Amino Acid Levels ◽  
Branched Chain

Beta-ketothiolase deficiency brought with lethargy: Case report

2011 ◽  
Vol 30 (10) ◽  
pp. 1724-1727 ◽  
Author(s):  
Vefik Arica ◽  
Secil Gunher Arica ◽  
Huseyin Dag ◽  
Hatice Onur ◽  
Ömer Obut ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Case Report ◽  
Blood Glucose ◽  
Amino Acid ◽  
Ketone Body ◽  
Autosomal Recessive ◽  
Autosomal Recessive Disorder ◽  
Branched Chain Amino Acid ◽  
Amino Acid Levels ◽  
Branched Chain

Beta-ketothiolase deficiency is a rare autosomal recessive disorder of isoleucine and ketone body metabolism. This disorder is clinically characterized by ketoacidotic attacks. Ketoacidosis, vomiting, and dehydration, lethargy and coma may be seen during attacks. A 9-month-old girl was admitted to our hospital with acidosis and dehydration. The patient was lethargic. Ketoacidosis was suspected because of acetone odor on her breath. Her blood glucose level was 262 mg/dL and urine ketone was (++++). Branched chain amino acid levels were elevated in her blood sample. Organic acid analysis of urine revealed 2-methylacetoacetyl-CoA thiolase deficiency. This was reported because of rarity of the disease and we should consider it in the differential diagnosis of ketoacidotic episodes.

scholarly journals Associations between branched chain amino acid levels, obesity and cardiometabolic complications

2015 ◽  
Vol 1 (6) ◽  
Author(s):  
Bénédicte Allam-Ndoul ◽  
Frédéric Guénard ◽  
Véronique Garneau ◽  
Olivier Barbier ◽  
Louis Pérusse ◽  
...  
Keyword(s):  
Amino Acid ◽  
Branched Chain Amino Acid ◽  
Amino Acid Levels ◽  
Branched Chain

A Comparison of the Effects of Intravenous Infusion of Individual Branched-Chain Amino Acids on Blood Amino Acid Levels in Man

1981 ◽  
Vol 60 (1) ◽  
pp. 95-100 ◽  
Author(s):  
S. Eriksson ◽  
L. Hagenfeldt ◽  
J. Wahren
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Intravenous Infusion ◽  
Serum Insulin ◽  
Aromatic Amino Acids ◽  
Branched Chain Amino Acids ◽  
Blood Concentrations ◽  
Branched Chain Amino Acid ◽  
Amino Acid Levels ◽  
Branched Chain

1., Intravenous infusions of l-valine (600 μmol/min), l-isoleucine (150 μmol/min), l-leucine (300 μmol/min) and a mixture of the three branched-chain amino acids (70% l-leucine, 20% l-valine, 10% l-isoleucine; 270 μmol/min) were given to four groups of healthy volunteer subjects. Whole-blood concentrations of amino acids and glucose and serum insulin were measured before and during the infusions. 2. Valine and isoleucine infusions resulted in twelve- and six-fold increases in the respective amino acid. During valine infusion, tyrosine was the only amino acid for which a decrease in concentration was seen (25%, P < 0.05). With isoleucine administration, no significant changes were found. In contrast, leucine infusion (during which the leucine concentration rose about sixfold) was accompanied by significant decreases in tyrosine (35%), phenylalanine (35%), methionine (50%), valine (40%) and isoleucine (55%). The arterial glucose concentration fell slightly (5%) and the insulin concentration increased 20% during leucine infusion. 3. Infusion of the mixture of the three branched-chain amino acids resulted in marked decreases in tyrosine (50%), phenylalanine (50%) and methionine (35%). The decreased amino acid levels remained low for 2 h after the end of the infusion. 4. The present findings demonstrate that intravenous infusion of leucine (not infusion of valine or isoleucine) results in marked reductions in the concentrations of the aromatic amino acids and methionine. Infusion of a mixture of the three branched-chain amino acids gives results similar to those obtained with leucine infusion alone. Thus a mixed branched-chain amino acid solution with leucine as its main constituent seems to be the best alternative in the treatment of patients with hepatic cirrhosis and encephalopathy.

scholarly journals Branched-chain amino acid levels are associated with improvement in insulin resistance with weight loss

Diabetologia ◽  
2011 ◽  
Vol 55 (2) ◽  
pp. 321-330 ◽  
Author(s):  
S. H. Shah ◽  
D. R. Crosslin ◽  
C. S. Haynes ◽  
S. Nelson ◽  
C. B. Turer ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Weight Loss ◽  
Amino Acid ◽  
Branched Chain Amino Acid ◽  
Amino Acid Levels ◽  
Branched Chain

Elevation of branched-chain amino acid levels in diabetes and NAFL and changes with antidiabetic drug treatment

2015 ◽  
Vol 9 (3) ◽  
pp. 293-297 ◽  
Author(s):  
Motoh Iwasa ◽  
Tomoaki Ishihara ◽  
Rumi Mifuji-Moroka ◽  
Naoki Fujita ◽  
Yoshinao Kobayashi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Drug Treatment ◽  
Antidiabetic Drug ◽  
Branched Chain Amino Acid ◽  
Amino Acid Levels ◽  
Branched Chain
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