scholarly journals A Point-of-Care Immunosensor Based on a Quartz Crystal Microbalance with Graphene Biointerface for Antibody Assay

ACS Sensors ◽  
2020 ◽  
Vol 5 (11) ◽  
pp. 3520-3532
Author(s):  
Piramon Hampitak ◽  
Thomas A. Jowitt ◽  
Daniel Melendrez ◽  
Maryline Fresquet ◽  
Patrick Hamilton ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Point Of Care ◽  
Antibody Assay

scholarly journals Blood Coagulation Testing Smartphone Platform Using Quartz Crystal Microbalance Dissipation Method

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 3073 ◽  
Author(s):  
Jia Yao ◽  
Bin Feng ◽  
Zhiqi Zhang ◽  
Chuanyu Li ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Clinical Laboratory ◽  
Point Of Care ◽  
Dissipation Factor ◽  
Parylene C ◽  
Adhesive Surface ◽  
Self Testing ◽  
Coagulation Testing

Blood coagulation function monitoring is important for people who are receiving anticoagulation treatment and a portable device is needed by these patients for blood coagulation self-testing. In this paper, a novel smartphone based blood coagulation test platform was proposed. It was developed based on parylene-C coated quartz crystal microbalance (QCM) dissipation measuring and analysis. The parylene-C coating constructed a robust and adhesive surface for fibrin capturing. The dissipation factor was obtained by measuring the frequency response of the sensor. All measured data were sent to a smartphone via Bluetooth for dissipation calculation and blood coagulation results computation. Two major coagulation indexes, activated partial thromboplastin time (APTT) and prothrombin time (PT) were measured on this platform compared with results by a commercial hemostasis system in a clinical laboratory. The measurement results showed that the adjusted R-square (R2) value for APTT and PT measurements were 0.985 and 0.961 respectively. The QCM dissipation method for blood coagulation measurement was reliable and effective and the platform together with the QCM dissipation method was a promising solution for point of care blood coagulation testing.

scholarly journals Rapid, Simple, and Sensitive Immunoagglutination Assay with SiO2 Particles and Quartz Crystal Microbalance for Quantifying Schistosoma japonicum Antibodies

2006 ◽  
Vol 52 (11) ◽  
pp. 2065-2071 ◽  
Author(s):  
Hua Wang ◽  
Yun Zhang ◽  
Bani Yan ◽  
Li Liu ◽  
Shiping Wang ◽  
...  
Keyword(s):  
Schistosoma Japonicum ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Point Of Care ◽  
Silica Particles ◽  
Rabbit Serum ◽  
Promising Alternative ◽  
Practical Applications ◽  
Sio2 Particles ◽  
Frequency Changes

Abstract Background: The resurgence of the parasitic disease schistosomiasis calls for more efficient diagnostic tests. We developed a rapid, simple, portable, and sensitive immunoagglutination assay that uses SiO2 particles and quartz crystal microbalance (QCM) for quantifying Schistosoma japonicum (Sj) antibodies (SjAb). Methods: We prepared submicrometer-sized silica particles derivatized with Sj antigens as replacements for traditional latex microspheres to specifically agglutinate in the presence of SjAb targets, and we used the QCM monitor to measure the resulting frequency shifts. We optimized the assay medium by adding poly(ethylene glycol) (PEG) as a response accelerator of immunoagglutination. To minimize or eliminate any nonspecific agglutination or adsorption interferences, we conducted appropriate sealing procedures separately for silica particles and the QCM probe. Results: The measured frequency changes were linearly related to the SjAb concentrations in infected rabbit serum. The PEG-assisted immunoagglutination system was quantitatively sensitive to SjAb concentrations ranging from ∼0.70 to 32.31 mg/L, with a detection limit of ∼0.46 mg/L. The obtained linear regression equation was: y = 43.61 x + 80.44 (r = 0.9872). Several serum specimens were evaluated with the developed QCM immunoassay and the results were compared with ELISA, validating the feasibility of practical applications. Conclusions: This novel immunoagglutination-based QCM detection format is rapid, simple to use, and more portable than conventional diagnostic immunoassays, thus offering a promising alternative tool that can be used for point-of-care clinical diagnosis of schistosomiasis, particularly in epidemic situations.

scholarly journals Verification of Quartz Crystal Microbalance Array using Vector Network Analyzer and OpenQCM

2018 ◽  
Vol 10 (1) ◽  
pp. 84 ◽  
Author(s):  
Ahmad Anwar Zainuddin ◽  
Anis Nurashikin Nordin ◽  
Rosminazuin Ab. Rahim ◽  
Aliza Aini Md. Ralib ◽  
Sheroz Khan ◽  
...  
Keyword(s):  
Resonance Frequency ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Point Of Care ◽  
Electrical Circuit ◽  
Vector Network Analyzer ◽  
Network Analyzer ◽  
Frequency Measurements ◽  
Percentage Difference ◽  
Resonance Frequencies

<p>Quartz Crystal Microbalance (QCM) is a device that allows non-destructive measurements of r in situ reaction activities. In this article, an array comprising of six 3MHz QCM sensors in an array were characterized using a vector network analyzer and OpenQCM, a portable measuring instrument that measures change in resonance frequency. Measurements of S21 transmission characteristics using the vector network analyzer provides the resonance frequency and can also be used to derive the RLC equivalent electrical circuit values of a resonant two-port network based on the Butterworth-Van Dyke model. In this work, R<sub>m</sub>, L<sub>m</sub>, C<sub>m</sub> and C<sub>o </sub>were obtained via curve-fitting of the measurement results to the simulated results. Measurements were done in triplicates to verify reproducibility for all 6 sensors. For comparison, measurements were also done using a portable, open-source instrument, OpenQCM. The OpenQCM instrument directly measures changes in resonance frequencies, making it ideal for biosensing experiments, which correlate changes in mass with changes in resonance frequencies. Comparison between resonance frequency measurements using VNA and OpenQCM exhibit low percentage difference 0.2%.  This QCM sensor array has the potential of conducting real-time, point-of-care analyses for detection of biological molecules. </p>

Interaction of Clear Flavor Emulsions Containing Lemon Essential Oils with Lipid Bilayers via a Quartz Crystal Microbalance

2019 ◽  
Vol 25 (6) ◽  
pp. 879-884
Author(s):  
Takahiro Sakai ◽  
Hayato Seki ◽  
Shogo Yoshida ◽  
Hayato Hori ◽  
Hisashi Suzuki ◽  
...  
Keyword(s):  
Essential Oils ◽  
Lipid Bilayers ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal

Effect of Current Distribution on Quartz Crystal Microbalance Measurements

1998 ◽  
Vol 145 (2) ◽  
pp. 492-497 ◽  
Author(s):  
James J. Kelly ◽  
K. M. Anisur Rahman ◽  
Christopher J. Durning ◽  
Alan C. West
Keyword(s):  
Quartz Crystal Microbalance ◽  
Current Distribution ◽  
Quartz Crystal
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