scholarly journals Live-Cell Surface-Enhanced Raman Spectroscopy Imaging of Intracellular pH: From Two Dimensions to Three Dimensions

ACS Sensors ◽  
2020 ◽  
Vol 5 (10) ◽  
pp. 3194-3206
Author(s):  
Yizhi Zhang ◽  
Dorleta Jimenez de Aberasturi ◽  
Malou Henriksen-Lacey ◽  
Judith Langer ◽  
Luis M. Liz-Marzán
Keyword(s):  
Raman Spectroscopy ◽  
Cell Surface ◽  
Intracellular Ph ◽  
Surface Enhanced Raman Spectroscopy ◽  
Two Dimensions ◽  
Live Cell ◽  
Three Dimensions ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Surface enhanced Raman spectroscopy (SERS) for the discrimination of Arthrobacter strains based on variations in cell surface composition

The Analyst ◽  
2012 ◽  
Vol 137 (18) ◽  
pp. 4280 ◽  
Author(s):  
Kate E. Stephen ◽  
Darren Homrighausen ◽  
Glen DePalma ◽  
Cindy H. Nakatsu ◽  
Joseph Irudayaraj
Keyword(s):  
Raman Spectroscopy ◽  
Cell Surface ◽  
Surface Composition ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Nanoprobes for intracellular and single cell surface-enhanced Raman spectroscopy (SERS)

2012 ◽  
Vol 43 (7) ◽  
pp. 817-827 ◽  
Author(s):  
Elina A. Vitol ◽  
Zulfiya Orynbayeva ◽  
Gary Friedman ◽  
Yury Gogotsi
Keyword(s):  
Raman Spectroscopy ◽  
Cell Surface ◽  
Single Cell ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Comparison of 4-Mercaptobenzoic Acid Surface-Enhanced Raman Spectroscopy-Based Methods for pH Determination in Cells

2020 ◽  
Vol 74 (11) ◽  
pp. 1423-1432
Author(s):  
Brian T. Scarpitti ◽  
Amy M. Morrison ◽  
Marina Buyanova ◽  
Zachary D. Schultz
Keyword(s):  
Raman Spectroscopy ◽  
Frequency Shift ◽  
Intracellular Ph ◽  
Surface Enhanced Raman Spectroscopy ◽  
Therapeutic Drug ◽  
High Signal ◽  
Signal To Noise ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
In Cells

Measurements of cellular pH are used to infer information such as stage of cell cycle, presence of cancer and other diseases, as well as delivery or effect of a therapeutic drug. Surface-enhanced Raman spectroscopy (SERS) of nanoparticle-based pH probes have been used to interrogate intracellular pH, with the significant advantage of avoiding photobleaching compared to fluorescent indicators. 4-Mercaptobenzoic acid (MBA) is a commonly used pH-sensitive reporter molecule. Intracellular pH sensing by SERS requires analysis of the observed MBA spectrum and spectral interference can affect the pH determination. Background from common cell containers, imaging too few particles, signal-to-noise ratios, and degradation of reporter molecules are among the factors that may alter appropriate SERS-based pH determination in cells. Here, we have compared common methods of spectral analysis to see how different factors alter the calculated pH in Raman maps of MBA functionalized Au nanostars in SW620 cancer cells. The methods included in our comparison use the relative intensity of the ν(COO–) stretch, chemometric analysis of the ν8a mode, and analyzing the frequency shift of the ν8a mode. These methods show different sensitivity to some of these sources of error in live cell experiments. pH determination based on Raman frequency shift appears to give a more reliable pH determination, though in high signal-to-noise environments, intensity ratios may provide better sensitivity to small changes in pH for cellular imaging.

Detection of Cell Surface Protein with Surface Enhanced Raman Spectroscopy

2006 ◽  
Vol 952 ◽  
Author(s):  
Li-Lin Tay ◽  
Qingyan Hu ◽  
Matthew Noestheden ◽  
John Pezacki
Keyword(s):  
Raman Spectroscopy ◽  
Cell Surface ◽  
Hela Cells ◽  
Transmembrane Domain ◽  
Surface Protein ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Proteins ◽  
Cell Surface Protein ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

ABSTRACTIn this study, HeLa cells were transfected to express a recombinant transmembrane domain of the platelet-derived growth factor receptor. Silver nanoparticle (Ag-NP) functionalized with terminal hydrazide was employed to specifically target the ketone-conjugated cell surface proteins. In addition, a nitrile functional group was incorporated onto the Ag nanoprobe to provide unique vibrational signature (∼ 2230 cm−1) in a window which is free from other cellular components. The designed Ag nanoprobe functions as both recognition and transduction elements in the detection of the expressed cell surface protein. Scanning electron microscopy and surface enhanced Raman spectroscopy (SERS) imaging of the nanoparticle (NP) labeled HeLa cells revealed that all the observed intense SERS signals originated from aggregated NPs. Although isolated Ag-NPs is capable of SERS enhancement, its enhancement factor is beyond the detestability of such imaging experiments. The results have implication in the future design of SERS nanosensors for in vivo detection application.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

Sign in / Sign up

Export Citation Format

Share Document