Ultrasensitive and Label-Free Detection of the Measles Virus Using an N-Heterocyclic Carbene-Based Electrochemical Biosensor

ACS Sensors ◽  
2020 ◽  
Vol 5 (9) ◽  
pp. 2747-2752
Author(s):  
Robert M. Mayall ◽  
Christene A. Smith ◽  
Alexander S. Hyla ◽  
Dianne S. Lee ◽  
Cathleen M. Crudden ◽  
...  
Keyword(s):  
Measles Virus ◽  
Electrochemical Biosensor ◽  
Label Free ◽  
Label Free Detection

Signal-on CoA-dependent electrochemical biosensor for highly sensitive and label-free detection of Citrate synthase activity

Talanta ◽  
2016 ◽  
Vol 161 ◽  
pp. 583-591 ◽  
Author(s):  
Qin Wang ◽  
Hongjun Chen ◽  
Yong Li ◽  
Huixia Wang ◽  
Zhou Nie ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Citrate Synthase ◽  
Label Free ◽  
Highly Sensitive ◽  
Label Free Detection

scholarly journals DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1125 ◽  
Author(s):  
Ana-Maria Chiorcea-Paquim ◽  
Ana Maria Oliveira-Brett
Keyword(s):  
Dna Damage ◽  
Electrode Surface ◽  
Oxidative Dna Damage ◽  
Electrochemical Biosensor ◽  
Electrochemical Biosensors ◽  
Label Free ◽  
Dna Electrochemical Biosensor ◽  
Wide Range ◽  
Label Free Detection

Deoxyribonucleic acid (DNA) electrochemical biosensors are devices that incorporate immobilized DNA as a molecular recognition element on the electrode surface, and enable probing in situ the oxidative DNA damage. A wide range of DNA electrochemical biosensor analytical and biotechnological applications in pharmacology are foreseen, due to their ability to determine in situ and in real-time the DNA interaction mechanisms with pharmaceutical drugs, as well as with their degradation products, redox reaction products, and metabolites, and due to their capacity to achieve quantitative electroanalytical evaluation of the drugs, with high sensitivity, short time of analysis, and low cost. This review presents the design and applications of label-free DNA electrochemical biosensors that use DNA direct electrochemical oxidation to detect oxidative DNA damage. The DNA electrochemical biosensor development, from the viewpoint of electrochemical and atomic force microscopy (AFM) characterization, and the bottom-up immobilization of DNA nanostructures at the electrode surface, are described. Applications of DNA electrochemical biosensors that enable the label-free detection of DNA interactions with pharmaceutical compounds, such as acridine derivatives, alkaloids, alkylating agents, alkylphosphocholines, antibiotics, antimetabolites, kinase inhibitors, immunomodulatory agents, metal complexes, nucleoside analogs, and phenolic compounds, which can be used in drug analysis and drug discovery, and may lead to future screening systems, are reviewed.

Facile electrochemical biosensor based on a new bifunctional probe for label-free detection of CGG trinucleotide repeat

2013 ◽  
Vol 49 ◽  
pp. 282-289 ◽  
Author(s):  
Hanping He ◽  
Jingping Xia ◽  
Xiaoqian Peng ◽  
Gang Chang ◽  
Xiuhua Zhang ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Trinucleotide Repeat ◽  
Label Free ◽  
Label Free Detection

High sensitive and selective electrochemical biosensor: Label-free detection of human norovirus using affinity peptide as molecular binder

2017 ◽  
Vol 87 ◽  
pp. 164-170 ◽  
Author(s):  
Hye Jin Hwang ◽  
Myung Yi Ryu ◽  
Chan Young Park ◽  
Junki Ahn ◽  
Hyun Gyu Park ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Label Free ◽  
Human Norovirus ◽  
Label Free Detection ◽  
Affinity Peptide

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

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