scholarly journals Fungitoxic effect of inorganic salts for the management of seed borne Macrophomina phaseolina and Fusarium sp. causing charcoal rot and wilt disease in blackgram

2019 ◽  
Author(s):  
N. Indra ◽  
A. S. Kauvyashree ◽  
D. S. Swetha ◽  
M. Asmina ◽  
. Shalini
Keyword(s):  
Sodium Bicarbonate ◽  
Potassium Chloride ◽  
Sodium Carbonate ◽  
Mycelial Growth ◽  
Potassium Phosphate ◽  
Ammonium Molybdate ◽  
Charcoal Rot ◽  
Potassium Phosphate Dibasic ◽  
Fusarium Sp

The laboratory experiments were carried out to study the effect of different salts viz., potassium chloride (KCl), potassium phosphate dibasic (K2HPO4), sodium carbonate (Na2CO3), sodium bicarbonate (NaHCO3) and ammonium molybdate (NH4)6 Mo7O24) on seed borne M. phaseolina and Fusarium sp. as an alternative to synthetic fungicides for the control of charcoal rot and wilt diseases in blackgram. The evaluation of different salts was performed in vitro using various concentrations viz., 0.5, 1.0, 2.0, 4.0 and 8.0 per cent (w/v). Among the salts tested against M. phaseolina, sodium carbonate, sodium bicarbonate and ammonium molybdate at 0.5, 2.0 and 4.0 per cent respectively inhibited the fungal growth. Among the salts tested against Fusarium sp. sodium carbonate (Na2CO3) at 4.0 per cent concentration recorded complete inhibition of the mycelial growth compared to the other salts. Also seed priming of these salts significantly reduced the seed borne infection due to M. phaseolina and Fusarium sp. under standard blotter test. The salts like potassium chloride and potassium phosphate dibasic (K2HPO4) at all concentrations did not inhibit M. phaseolina and Fusarium sp. which recorded 100 per cent mycelial growth as that of control.

scholarly journals A Novel Analytical Method for Simultaneous Quantification of Silodosin and Tadalafil by RP-HPLC

2021 ◽  
pp. 193-202
Author(s):  
Ajay Gupta ◽  
S. K. Mishra
Keyword(s):  
Analytical Method ◽  
Potassium Phosphate ◽  
Cost Effective ◽  
Hplc Method ◽  
Simultaneous Quantification ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Accuracy Parameter ◽  
Potassium Phosphate Dibasic

The Reverse phase HPLC method was developed for simultaneous determination of Silodosin and Tadalafil in single analytical method. Chromatographic separation was achieved on a Supelco C8 (150mmx4.6mm, 5µm) column applying an isocratic elution based on premix of potassium phosphate dibasic buffer pH (4.3) and acetonitrile in the ratio of (70:30 v/v) as mobile. Validation parameters specificity, precision and robustness have been observed to be desirable over the concentration ranges of 80-240 µg/ml for Silodosin and 50-150 µg/ml for Tadalafil in accuracy parameter and 128-192 µg/ml for Silodosin and 80-120 µg/ml for Tadalafil in linearity parameter. All the variables have been studied to optimize the chromatographic conditions. The optimized approach verified through validation and confirmed to be intended purpose for the quality control of the mentioned drugs, as per ICH guidelines. For simultaneous quantification of Silodosin and Tadalafil, the developed method was found to be genuinely exact precise, accurate, linear, fast and cost effective.

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