Marker-Free Automatic Quantification of Drug-Treated Cardiomyocytes with Digital Holographic Imaging

ACS Photonics ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Keyvan Jaferzadeh ◽  
Benjamin Rappaz ◽  
Fabien Kuttler ◽  
Bo kyoung Kim ◽  
Inkyu Moon ◽  
...  
Keyword(s):  
Automatic Quantification ◽  
Holographic Imaging ◽  
Marker Free

Performance and applications of the holography electron microscope

1993 ◽  
Vol 51 ◽  
pp. 1078-1079
Author(s):  
Akira Tonomura
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Phase Measurement ◽  
Electron Holography ◽  
Imaging Method ◽  
High Contrast ◽  
Magnetic Lens ◽  
High Brightness ◽  
Holographic Imaging ◽  
Dynamic Observation

Electron holography is a two-step imaging method. However, the ultimate performance of holographic imaging is mainly determined by the brightness of the electron beam used in the hologram-formation process. In our 350kV holography electron microscope (see Fig. 1), the decrease in the inherently high brightness of field-emitted electrons is minimized by superposing a magnetic lens in the gun, for a resulting value of 2 × 109 A/cm2 sr. This high brightness has lead to the following distinguished features. The minimum spacing (d) of carrier fringes is d = 0.09 Å, thus allowing a reconstructed image with a resolution, at least in principle, as high as 3d=0.3 Å. The precision in phase measurement can be as high as 2π/100, since the position of fringes can be known precisely from a high-contrast hologram formed under highly collimated illumination. Dynamic observation becomes possible because the current density is high.

Production of γ-Linolenic Acid and Stearidonic Acid in Seeds of Marker-Free Transgenic Soybean

Crop Science ◽  
2004 ◽  
Vol 44 (2) ◽  
pp. 646 ◽  
Author(s):  
Shirley Sato ◽  
Aiqiu Xing ◽  
Xingguo Ye ◽  
Bruce Schweiger ◽  
Anthony Kinney ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Stearidonic Acid ◽  
Transgenic Soybean ◽  
Marker Free

scholarly journals Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic particle-mediated co-transformation

2017 ◽  
Vol 5 (4) ◽  
pp. 271-281 ◽  
Author(s):  
Dan Feng ◽  
Yanwei Wang ◽  
Jinxia Wu ◽  
Tiegang Lu ◽  
Zhiguo Zhang
Keyword(s):  
Drought Tolerance ◽  
Transgenic Rice ◽  
Marker Free

Rapid visualized assessment of drug efficacy in live mice with a selectable marker-free autoluminescent Klebsiella pneumoniae

2021 ◽  
Vol 177 ◽  
pp. 112919
Author(s):  
Xirong Tian ◽  
Yamin Gao ◽  
Shuai Wang ◽  
H.M. Adnan Hameed ◽  
Wei Yu ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Selectable Marker ◽  
Drug Efficacy ◽  
Marker Free
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