scholarly journals Identification and Characterization of a DmoB Flavin Oxidoreductase from a Putative Two-Component DMS C-Monooxygenase

ACS Omega ◽  
2020 ◽  
Vol 5 (17) ◽  
pp. 9830-9838
Author(s):  
D. Scott Hammers ◽  
Caroline M. Donaghy ◽  
Sarah L. Heiss ◽  
Lydia M. Harris ◽  
Jackson M. Gordon ◽  
...  
Keyword(s):  
Two Component ◽  
Flavin Oxidoreductase ◽  
Identification And Characterization

Identification and Characterization of the Two-Component System HK8700–RR8701 of Kocuria rhizophila DC2201

2019 ◽  
Vol 38 (6) ◽  
pp. 683-692
Author(s):  
Baohua Chen ◽  
Tingting Zou ◽  
Long Zou ◽  
Haiyan Ni ◽  
Yunhong Huang ◽  
...  
Keyword(s):  
Two Component System ◽  
Component System ◽  
Two Component ◽  
Identification And Characterization ◽  
Kocuria Rhizophila

scholarly journals Identification and characterization of KvgAS, a two-component system inKlebsiella pneumoniaeCG43

2003 ◽  
Vol 218 (1) ◽  
pp. 121-126 ◽  
Author(s):  
Yi-Chyi Lai ◽  
Ging-Ting Lin ◽  
Shu-Li Yang ◽  
Hwan-You Chang ◽  
Hwei-Ling Peng
Keyword(s):  
Two Component System ◽  
Component System ◽  
Two Component ◽  
Identification And Characterization

scholarly journals Candida albicans SRR1 , a Putative Two-Component Response Regulator Gene, Is Required for Stress Adaptation, Morphogenesis, and Virulence

2011 ◽  
Vol 10 (10) ◽  
pp. 1370-1374 ◽  
Author(s):  
Chirayu Desai ◽  
John Mavrianos ◽  
Neeraj Chauhan
Keyword(s):  
Candida Albicans ◽  
Response Regulator ◽  
Stress Adaptation ◽  
Regulator Gene ◽  
Content Type ◽  
Virulence Attenuation ◽  
Two Component ◽  
Resistance To Stress ◽  
Identification And Characterization

ABSTRACT We report here the identification and characterization of a previously uncharacterized, two-component response regulator gene (orf19.5843) from Candida albicans . Because of its apparent functions in stress adaptation, we have named this gene SRR1 ( s tress r esponse r egulator 1). Disruption of SRR1 causes defects in hyphal development, reduced resistance to stress, and severe virulence attenuation in the mouse model of disseminated candidiasis.

scholarly journals Identification and Characterization of the Flavin:NADH Reductase (PrnF) Involved in a Novel Two-Component Arylamine Oxygenase

2007 ◽  
Vol 189 (23) ◽  
pp. 8556-8563 ◽  
Author(s):  
Jung-Kul Lee ◽  
Huimin Zhao
Keyword(s):  
Escherichia Coli ◽  
Flavin Adenine Dinucleotide ◽  
Whole Genome ◽  
Flavin Reductase ◽  
Oxidation Reactions ◽  
Protein Protein Interaction ◽  
Two Component ◽  
Identification And Characterization ◽  
Efficient Transfer

ABSTRACT Two-component oxygenases catalyze a wide variety of important oxidation reactions. Recently we characterized a novel arylamine N-oxygenase (PrnD), a new member of the two-component oxygenase family (J. Lee et al., J. Biol. Chem. 280:36719-36728, 2005). Although arylamine N-oxygenases are widespread in nature, aminopyrrolnitrin N-oxygenase (PrnD) represents the only biochemically and mechanistically characterized arylamine N-oxygenase to date. Here we report the use of bioinformatic and biochemical tools to identify and characterize the reductase component (PrnF) involved in the PrnD-catalyzed unusual arylamine oxidation. The prnF gene was identified via sequence analysis of the whole genome of Pseudomonas fluorescens Pf-5 and subsequently cloned and overexpressed in Escherichia coli. The purified PrnF protein catalyzes reduction of flavin adenine dinucleotide (FAD) by NADH with a k cat of 65 s−1 (K m = 3.2 μM for FAD and 43.1 μM for NADH) and supplies reduced FAD to the PrnD oxygenase component. Unlike other known reductases in two-component oxygenase systems, PrnF strictly requires NADH as an electron donor to reduce FAD and requires unusual protein-protein interaction with the PrnD component for the efficient transfer of reduced FAD. This PrnF enzyme represents the first cloned and characterized flavin reductase component in a novel two-component arylamine oxygenase system.

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