scholarly journals Structural Characterization of the N-Terminal Domain of the Dictyostelium discoideum Mitochondrial Calcium Uniporter

ACS Omega ◽  
2020 ◽  
Vol 5 (12) ◽  
pp. 6452-6460 ◽  
Author(s):  
Yuan Yuan ◽  
Chan Cao ◽  
Maorong Wen ◽  
Min Li ◽  
Ying Dong ◽  
...  
Keyword(s):  
Dictyostelium Discoideum ◽  
Structural Characterization ◽  
Mitochondrial Calcium ◽  
Mitochondrial Calcium Uniporter ◽  
Terminal Domain ◽  
Calcium Uniporter

scholarly journals Structural characterization of the N-terminal domain of the Dictyostelium discoideum mitochondrial calcium uniporter

2019 ◽  
Author(s):  
Yuan Yuan ◽  
Chan Cao ◽  
Maorong Wen ◽  
Min Li ◽  
Ying Dong ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dictyostelium Discoideum ◽  
Self Assembly ◽  
Calcium Influx ◽  
Transmembrane Protein ◽  
Critical Role ◽  
Mitochondrial Calcium ◽  
Mitochondrial Calcium Uniporter ◽  
Terminal Domain ◽  
Calcium Uniporter

AbstractThe mitochondrial calcium uniporter (MCU) plays a critical role in the mitochondrial calcium uptake into the matrix. In metazoans, the uniporter is a tightly regulated multi-component system including the pore-forming subunit MCU and several regulators (MICU1, MICU2, EMRE). The calcium-conducting activity of metazoan MCU requires the single-transmembrane protein EMRE. Dictyostelium discoideum (Dd), however, developed a simplified uniporter for which the pore-forming MCU (DdMCU) alone is necessary and sufficient for calcium influx. Here, we report a crystal structure of the N-terminal domain (NTD) of DdMCU at 1.7 Å resolution. The DdMCU-NTD contains four helices and two strands arranged in a fold that is completely different from the known structures of other MCU-NTD homologs. Biochemical and biophysical analyses of DdMCU-NTD in solution indicated that the domain exists as oligomers, most probably as a pentamer or hexamer. Mutagenesis showed that the acidic residues Asp60, Glu72 and Glu74, which appeared to mediate the parallel interface as observed in the crystal structure, participated in the self-assembly of DdMCU-NTD. Intriguingly, the oligomeric complex readily dissociated to lower-order oligomers in the presence of calcium. We propose that the calcium-triggered dissociation of NTD regulates the channel activity of DdMCU by a yet unknown mechanism.

Purification of the mitochondrial calcium uniporter from beef heart and characterization of its properties

BIOPHYSICS ◽  
2010 ◽  
Vol 55 (5) ◽  
pp. 718-722
Author(s):  
E. N. Gritsenko ◽  
V. P. Kutyshenko ◽  
N. -E. L. Saris ◽  
M. Wahlsten ◽  
J. Jokela ◽  
...  
Keyword(s):  
Mitochondrial Calcium ◽  
Beef Heart ◽  
Mitochondrial Calcium Uniporter ◽  
Calcium Uniporter

scholarly journals Electrophysiological Characterization of the Activity and Regulation of the Mitochondrial Calcium Uniporter

2014 ◽  
Vol 106 (2) ◽  
pp. 760a
Author(s):  
Vanessa Checchetto ◽  
Enrico Teardo ◽  
Diego De Stefani ◽  
Maria Patron ◽  
Anna Raffaello ◽  
...  
Keyword(s):  
Mitochondrial Calcium ◽  
Mitochondrial Calcium Uniporter ◽  
Calcium Uniporter ◽  
Electrophysiological Characterization

NMR structural characterization of the N-terminal domain of the adenylyl cyclase-associated protein (CAP) from Dictyostelium discoideum

2004 ◽  
Vol 29 (1) ◽  
pp. 73-84 ◽  
Author(s):  
Chrystelle Mavoungou ◽  
Lars Israel ◽  
Till Rehm ◽  
Dorota Ksiazek ◽  
Marcin Krajewski ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Dictyostelium Discoideum ◽  
Structural Characterization ◽  
Terminal Domain

scholarly journals Novel Approach for The Electrophysiological Characterization of Mitochondrial Calcium Uniporter

2021 ◽  
Vol 35 (S1) ◽  
Author(s):  
Iuliia Polina ◽  
An Xie ◽  
Jyotsna Mishra ◽  
Neeta Adhikari ◽  
Richard Clements ◽  
...  
Keyword(s):  
Mitochondrial Calcium ◽  
Mitochondrial Calcium Uniporter ◽  
Calcium Uniporter ◽  
Novel Approach ◽  
Electrophysiological Characterization

scholarly journals Physiological Characterization of a Plant Mitochondrial Calcium Uniporter in Vitro and in Vivo

2016 ◽  
Vol 173 (2) ◽  
pp. 1355-1370 ◽  
Author(s):  
Enrico Teardo ◽  
Luca Carraretto ◽  
Stephan Wagner ◽  
Elide Formentin ◽  
Smrutisanjita Behera ◽  
...  
Keyword(s):  
Mitochondrial Calcium ◽  
Mitochondrial Calcium Uniporter ◽  
Physiological Characterization ◽  
Calcium Uniporter

scholarly journals Structure and function of the N‐terminal domain of the human mitochondrial calcium uniporter

EMBO Reports ◽  
2015 ◽  
Vol 16 (10) ◽  
pp. 1318-1333 ◽  
Author(s):  
Youngjin Lee ◽  
Choon Kee Min ◽  
Tae Gyun Kim ◽  
Hong Ki Song ◽  
Yunki Lim ◽  
...  
Keyword(s):  
Structure And Function ◽  
Mitochondrial Calcium ◽  
Mitochondrial Calcium Uniporter ◽  
Terminal Domain ◽  
Calcium Uniporter ◽  
And Function

288-LB: Effects of the Hepatic Mitochondrial Calcium Uniporter on Hepatic Gluconeogenesis and Mitochondrial Metabolism in Awake Mice

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 288-LB
Author(s):  
JI EUN LEE ◽  
LEIGH GOEDEKE ◽  
YE ZHANG ◽  
RACHEL J. PERRY ◽  
RUSSELL GOODMAN ◽  
...  
Keyword(s):  
Mitochondrial Metabolism ◽  
Mitochondrial Calcium ◽  
Hepatic Gluconeogenesis ◽  
Mitochondrial Calcium Uniporter ◽  
Calcium Uniporter
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