scholarly journals Mass Spectrometric Imaging of the Brain Demonstrates the Regional Displacement of 6-Monoacetylmorphine by Naloxone

ACS Omega ◽  
2020 ◽  
Vol 5 (22) ◽  
pp. 12596-12602
Author(s):  
Belin G. Teklezgi ◽  
Annapurna Pamreddy ◽  
Sphamandla Ntshangase ◽  
Sipho Mdanda ◽  
Sanil D. Singh ◽  
...  
Keyword(s):  
Mass Spectrometric ◽  
Mass Spectrometric Imaging ◽  
The Brain

Investigating time dependent brain distribution of nevirapine via mass spectrometric imaging

2019 ◽  
Vol 50 (6) ◽  
pp. 593-599
Author(s):  
Sipho Mdanda ◽  
Sphamandla Ntshangase ◽  
Sanil D. Singh ◽  
Tricia Naicker ◽  
Hendrik G. Kruger ◽  
...  
Keyword(s):  
Mass Spectrometric ◽  
Time Dependent ◽  
Brain Distribution ◽  
Mass Spectrometric Imaging

MO430MASS-SPECTROMETRIC IDENTIFICATION OF POST-TRANSLATIONAL GUANIDINYLATED PROTEINS IN THE CONTEXT OF SYSTEMIC LUPUS ERYTHEMATOSUS

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Juliane Hermann ◽  
Ute Raffetseder ◽  
Michaela Lellig ◽  
Joachim Jankowski ◽  
Vera Jankowski
Keyword(s):  
Mass Spectrometry ◽  
Systemic Lupus Erythematosus ◽  
High Resolution ◽  
Lupus Erythematosus ◽  
Imaging Techniques ◽  
Mass Spectrometric ◽  
Mass Spectrometric Imaging ◽  
Systemic Lupus ◽  
Notch 3 ◽  
Resolution Mass

Abstract Background and Aims With continuous identification of post-translational modified isoforms of proteins, it is becoming increasingly clear that post-translational modifications limit or modify the biological functions of native proteins are majorly involved in development of various chronic disease. This is mostly due to technically advanced molecular identification and quantification methods, mainly based on mass spectrometry. Mass spectrometry has become one of the most powerful tools for the identification of lipids. Method In this study, we used sophisticated high-resolution mass-spectrometric methods to analyze the soluble ligand of receptor Notch-3, namely the Y-box protein (YB)-1, in serum from systemic lupus erythematosus (SLE) patients. In addition, kidneys of lupus-prone (MRL.lpr) mice were analyzed by mass-spectrometric imaging techniques to identify the underlying pathomechanisms. Serum YB-1 was isolated by chromatographic methods, afterwards digested by trypsin and analyzed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). The kidneys were fixed in paraffin, then kidney sections were deparaffinized, tryptic digested and analyzed by mass-spectrometric imaging techniques. Mass-spectrometry of extracellular YB-1 in SLE patient serum revealed post-translational guanidinylation of two lysine’s within the highly conserved cold shock domain (CSD) of the YB-1 protein (YB-1-2G). Patients with increased disease activity and those with active renal involvement (lupus nephritis, LN) had a higher degree of dual-guanidinylation within the CSD. Of note, at least one of these modifications was present in all analyzed LN patients, whereas single-guanidinylated YB-1 was present in only one and double modification in none of the control individuals. Mass-spectrometric imaging analyses specifically localized YB-1-2G and increases Notch-3 expression in kidney sections from MRL.lpr mice. Results The data from this study clearly demonstrate the high potential of high-resolution mass spectrometric methods as well as mass spectrometric imaging techniques to identify pathomechanisms of diseases like SLE/LN.

Structural and Mass Spectrometric Imaging Analyses of Adhered Tunic and Adhesive Projections of Solitary Ascidians

2018 ◽  
Vol 35 (6) ◽  
pp. 535-547 ◽  
Author(s):  
Tatsuya Ueki ◽  
Kanae Koike ◽  
Ikuko Fukuba ◽  
Nobuo Yamaguchi
Keyword(s):  
Mass Spectrometric ◽  
Mass Spectrometric Imaging

Optimization and evaluation of MALDI TOF mass spectrometric imaging for quantification of orally dosed octreotide in mouse tissues

Talanta ◽  
2017 ◽  
Vol 165 ◽  
pp. 128-135 ◽  
Author(s):  
Tai Rao ◽  
Boyu Shen ◽  
Zhangpei Zhu ◽  
Yuhao Shao ◽  
Dian Kang ◽  
...  
Keyword(s):  
Mass Spectrometric ◽  
Mass Spectrometric Imaging ◽  
Maldi Tof ◽  
Mouse Tissues

scholarly journals Mass spectrometric imaging of lipids using desorption electrospray ionization

2009 ◽  
Vol 877 (26) ◽  
pp. 2883-2889 ◽  
Author(s):  
Allison L. Dill ◽  
Demian R. Ifa ◽  
Nicholas E. Manicke ◽  
Zheng Ouyang ◽  
R. Graham Cooks
Keyword(s):  
Electrospray Ionization ◽  
Desorption Electrospray Ionization ◽  
Mass Spectrometric ◽  
Mass Spectrometric Imaging

scholarly journals Nanomaterials and continuous wave laser-based efficient desorption for atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices

RSC Advances ◽  
2018 ◽  
Vol 8 (15) ◽  
pp. 8021-8025 ◽  
Author(s):  
Jae Young Kim ◽  
Eun Seok Seo ◽  
Hee Jin Lim ◽  
Hyunmin Kim ◽  
Ji-Won Park ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Continuous Wave ◽  
Mass Spectrometric ◽  
Continuous Wave Laser ◽  
Mass Spectrometric Imaging ◽  
Tissue Slices ◽  
Hippocampal Tissue ◽  
Wave Laser ◽  
532 Nm ◽  
Resolution Mass

Micrometer-resolution mass spectrometric imaging of live hippocampal tissue is achieved with a highly efficient desorption of biomolecules using a 532 nm continuous wave laser and gold nanoparticles or graphene oxide as an energy transporter.

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