scholarly journals Combined Use of Scanning Electron Microscopy–Energy-Dispersive X-ray Spectroscopy and Fourier Transform Infrared Imaging Coupled with Principal Component Analysis in the Study of Ancient Egyptian Papyri

ACS Omega ◽  
2019 ◽  
Vol 4 (26) ◽  
pp. 22041-22047 ◽  
Author(s):  
Cinzia Chiappe ◽  
Christian Silvio Pomelli ◽  
Stefania Sartini
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Principal Component Analysis ◽  
Infrared Imaging ◽  
Principal Component ◽  
X Ray ◽  
Ancient Egyptian ◽  
Combined Use ◽  
Fourier Transform Infrared Imaging ◽  
Scanning Electron

Kaolinite dispersion in cassava starch-based composite films: a photonic microscopy and X-ray tomography study

2018 ◽  
Vol 38 (7) ◽  
pp. 641-647
Author(s):  
Jean Aimé Mbey ◽  
Fabien Thomas ◽  
Sandrine Hoppe
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Dimethyl Sulfoxide ◽  
Polymer Matrix ◽  
Composite Films ◽  
Cassava Starch ◽  
X Ray ◽  
Clay Dispersion ◽  
Combined Use ◽  
Scanning Electron

Abstract In the present study, a combined use of photonic microscopy, scanning electron microscopy and 3D X-ray tomography is carried out in order to analyze the dispersion and the distribution of raw and dimethyl sulfoxide (DMSO)-intercalated kaolinite used as filler in cassava starch-based films. It is shown that the association of these techniques allows a valuable analysis of clay dispersion in polymer-clay composite films. In the case of kaolinite-starch composite films on which this study is focused, it is obvious that previous intercalation of kaolinite with DMSO is an efficient way to improve dispersion and distribution of kaolinite in a starch polymer matrix.

Scanning Electron Microscopy and Geometric Contour Morphometry for Identifying Eggs of Three Amazonian Species of Mansonia (Diptera: Culicidae)

2019 ◽  
Vol 57 (3) ◽  
pp. 745-754
Author(s):  
Francisco Augusto da Silva Ferreira ◽  
Rejane de Castro Simões ◽  
Ruth Leila Ferreira-Keppler ◽  
Jeronimo Alencar ◽  
Vera Margarete Scarpassa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Principal Component Analysis ◽  
Principal Component ◽  
Accuracy Rate ◽  
Morphometric Characters ◽  
Geometric Morphometry ◽  
Neotropical Species ◽  
Egg Morphology ◽  
Scanning Electron

Abstract The eggs of three Amazonian species of the genus Mansonia (Diptera: Culicidae) were analyzed using morphological and morphometric characters. Eggs of Mansonia humeralis Dyar & Knab, 1916 were morphologically different from those of Mansonia titillans (Walker, 1848) and Mansonia amazonensis (Theobald, 1901), which were more similar to each other according to linear and geometric morphometry. A principal component analysis generated from elliptic Fourier contour data (PC1—92.6% and PC2—2.61%) indicated that Ma. amazonensis and Ma. titillans are more similar to each other than either is to Ma. humeralis. Discriminant multivariate analysis was highly accurate with only four classification errors and a 90% accuracy rate. The results indicate that the three Amazonian species can be precisely distinguished in the egg stage and that geometric morphometry based on elliptic Fourier contours is a promising method for distinguishing eggs of species of Mansonia. An identification key based on egg morphology is provided to distinguish the four Neotropical species.

scholarly journals Comparative morphology and anatomy of seeds of some Aethionema W.T. Aiton (Brassicaceae) taxa from Turkey

2019 ◽  
Vol 26 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Mehmet Cengiz Karaismailoğlu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Principal Component Analysis ◽  
Principal Component ◽  
Comparative Morphology ◽  
Seed Morphology ◽  
Anatomical Features ◽  
Major Significance ◽  
Surface Patterns ◽  
Scanning Electron

Seed morphology and anatomy are taxonomically significant in Brassicaceae. The seed structures of Aethionema from Turkey, which include 12 taxa (Aethionema syriacum, A. froedinii, A. arabicum, A. eunomioides, A. fimbriatum, A. speciosum subsp. speciosum, A. speciosum subsp. compactum, A. saxatile, A. oppositifolium, A. iberideum, A. armenum, A. grandiflorum) were studied for knowing seed morphological and anatomical features with one-way analysis of variance, cluster analysis and principal component analysis. Seed size, shape and color were examined with stereomicroscopy.The surface patterns of seed were observed using Scanning Electron Microscopy (SEM). In addition, structure, and thicknesses of testa and endosperm were investigated anatomically. Thickness of testa and endosperm were of major significance to illustrate interspecific relations among the examined taxa.

Identification of calcium oxalate crystals in dried or fixed tobacco leaf

1984 ◽  
Vol 42 ◽  
pp. 288-289
Author(s):  
Vicki L. Baliga ◽  
Mary Ellen Counts
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Calcium Oxalate ◽  
Growth And Development ◽  
Polarized Light ◽  
Calcium Oxalate Crystals ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

Calcium is an important element in the growth and development of plants and one form of calcium is calcium oxalate. Calcium oxalate has been found in leaf seed, stem material plant tissue culture, fungi and lichen using one or more of the following methods—polarized light microscopy (PLM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and x-ray diffraction.Two methods are presented here for qualitatively estimating calcium oxalate in dried or fixed tobacco (Nicotiana) leaf from different stalk positions using PLM. SEM, coupled with energy dispersive x-ray spectrometry (EDS), and powder x-ray diffraction were used to verify that the crystals observed in the dried leaf with PLM were calcium oxalate.

Scanning Electron Microscopy and X-Ray Analyses of Dental Tissues from a Hibernator

1976 ◽  
Vol 34 ◽  
pp. 178-179
Author(s):  
M. L. Zimny ◽  
A. C. Haller
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Body Temperature ◽  
Ground Squirrel ◽  
Alveolar Bone ◽  
X Ray ◽  
Dental Tissues ◽  
Bone Minerals ◽  
Apical Tooth ◽  
Scanning Electron

During hibernation the ground squirrel is immobile, body temperature reduced and metabolism depressed. Hibernation has been shown to affect dental tissues varying degrees, although not much work has been done in this area. In limited studies, it has been shown that hibernation results in (1) mobilization of bone minerals; (2) deficient dentinogenesis and degeneration of alveolar bone; (3) presence of calculus and tears in the cementum; and (4) aggrevation of caries and pulpal and apical tooth abscesses. The purpose of this investigation was to study the effects of hibernation on dental tissues employing scanning electron microscopy (SEM) and related x-ray analyses.

Scanning Electron Microscopy and x-ray analysis of microparticles and early hydration effects

1995 ◽  
Vol 53 ◽  
pp. 692-693
Author(s):  
Yun Lu ◽  
David C. Joy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Morphological Development ◽  
Early Hydration ◽  
X Ray ◽  
Blended Cements ◽  
Powder Particles ◽  
Chemical Wastes ◽  
Scanning Electron

High resolution scanning electron microscopy (SEM) and energy dispersive x-ray analysis (EDXA) were performed to investigate microparticles in blended cements and their hydration products containing sodium-rich chemical wastes. The physical appearance of powder particles and the morphological development at different hydration stages were characterized by using high resolution SEM Hitachi S-900 and by SEM S-800 with a EDX spectrometer. Microparticles were dispersed on the sample holder and glued by 1% palomino solution. Hydrated bulk samples were dehydrated by acetone and mounted on the holder by silver paste. Both fracture surfaces and flat cutting sections of hydrating samples were prepared and examined. Some specimens were coated with an 3 nm thick Au-Pd or Cr layer to provide good conducting surfaces. For high resolution SEM S-900 observations the accelerating voltage of electrons was 1-2 KeV to protect the electron charging. Microchemical analyses were carried out by S800/EDS equipped with a LINK detector of take-off angle =40°.

Defects of Platelet Function Recognized by X-Ray Imaging and Scanning Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 610-613
Author(s):  
M.G. Baldini ◽  
S. Morinaga ◽  
D. Minasian ◽  
R. Feder ◽  
D. Sayre ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Biology ◽  
Imaging Technique ◽  
Human Platelets ◽  
X Rays ◽  
X Ray ◽  
X Ray Imaging ◽  
Complex Phenomena ◽  
Scanning Electron

Contact X-ray imaging is presently developing as an important imaging technique in cell biology. Our recent studies on human platelets have demonstrated that the cytoskeleton of these cells contains photondense structures which can preferentially be imaged by soft X-ray imaging. Our present research has dealt with platelet activation, i.e., the complex phenomena which precede platelet appregation and are associated with profound changes in platelet cytoskeleton. Human platelets suspended in plasma were used. Whole cell mounts were fixed and dehydrated, then exposed to a stationary source of soft X-rays as previously described. Developed replicas and respective grids were studied by scanning electron microscopy (SEM).

Environmental scanning electron microscopy of fresh human gallstones reveals new morphologies of precipitated calcium salts

1992 ◽  
Vol 50 (2) ◽  
pp. 1322-1323
Author(s):  
Howard S. Kaufman ◽  
Keith D. Lillemoe ◽  
John T. Mastovich ◽  
Henry A. Pitt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Environmental Scanning Electron Microscopy ◽  
Environmental Scanning ◽  
X Ray Diffraction ◽  
Calcium Salts ◽  
Cholesterol Gallstones ◽  
X Ray ◽  
Ca Carbonate ◽  
Scanning Electron

Gallstones contain precipitated cholesterol, calcium salts, and proteins. Calcium (Ca) bilirubinate, palmitate, phosphate, and carbonate occurring in gallstones have variable morphologies but characteristic windowless energy dispersive x-ray (EDX) spectra. Previous studies of gallstone microstructure and composition using scanning electron microscopy (SEM) with EDX have been limited to dehydrated samples. In this state, Ca bilirubinates appear as either glassy masses, which predominate in black pigment stones, or as clusters, which are found mostly in cholesterol gallstones. The three polymorphs of Ca carbonate, calcite, vaterite, and aragonite, have been identified in gallstones by x-ray diffraction, however; the morphologies of these crystals vary in the literature. The purpose of this experiment was to study fresh gallstones by environmental SEM (ESEM) to determine if dehydration affects gallstone Ca salt morphology.Gallstones and bile were obtained fresh at cholecystectomy from 6 patients. To prevent dehydration, stones were stored in bile at 37°C. All samples were studied within 4 days of procurement.

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