scholarly journals New Polylactic Acid Multifunctional Ultrasound Contrast Agent Based on Graphene Oxide as the Carrier of Targeted Factor and Drug Delivery

ACS Omega ◽  
2019 ◽  
Vol 4 (3) ◽  
pp. 4691-4696
Author(s):  
Jie Zhang ◽  
Limei Song ◽  
Huiming Zhang ◽  
Shujing Zhou ◽  
Yufeng Jiao ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Graphene Oxide ◽  
Contrast Agent ◽  
Polylactic Acid ◽  
Ultrasound Contrast Agent ◽  
Agent Based ◽  
Ultrasound Contrast

scholarly journals Novel Span-PEG Multifunctional Ultrasound Contrast Agent Based on CNTs as a Magnetic Targeting Factor and a Drug Carrier

ACS Omega ◽  
2020 ◽  
Vol 5 (49) ◽  
pp. 31525-31534
Author(s):  
Jie Zhang ◽  
Zhongtao Liu ◽  
Shujing Zhou ◽  
Yang Teng ◽  
Xiangyu Zhang ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Drug Carrier ◽  
Ultrasound Contrast Agent ◽  
Magnetic Targeting ◽  
Agent Based ◽  
Ultrasound Contrast

Inducing Tumor Cell Apoptosis: Mediated Survivin miRNA by Ultrasound and Cationic Lipid Contrast Agent

2020 ◽  
Vol 20 ◽  
Author(s):  
Feng Yang ◽  
Liu-Fu Chun ◽  
Zhi-Yi Chen
Keyword(s):  
Drug Delivery ◽  
Contrast Agent ◽  
Hela Cells ◽  
Cell Apoptosis ◽  
Transfection Efficiency ◽  
Ultrasound Contrast Agent ◽  
Chemical Properties ◽  
Cationic Lipid ◽  
Viral Gene ◽  
Ultrasound Contrast

Background: Non-viral delivery systems is a promising method for gene or drug delivery. Polyethyleneimine(PEI) is a double-edged sword. It internalizes itself into the cell through endocytosis and promotes gene transfer efficiency. However, the large positive charge also makes PEI highly toxic to cells. Ultrasound-targeted microbubble destruction (UTMD) is a promising non-viral method for gene and drug delivery, but its efficiency still needs to be improved. Objective: The aim of this study was to explore a system that combines ultrasound with non-viral gene delivery for the treatment of cervical cancer HeLa cells. Methods: In this study, we synthesized a kind of cationic ultrasound contrast agent(C-UCA)that the physical and chemical properties, gene carrying capacity and cytotoxicity were verified. On the basis of previous studies, we further optimized the following transfusion parameters including ultrasound parameters, microbubble concentration, plasmid concentration, cell density and other parameters. The experiment was designed to compare the following six groups: (1) Plasmid group (P group), plasmid 15 μg; (2) PEI + plasmid group (PEI + P group),1 μl of PEI containing 10 nmol nitrogen and 1 μg of DNA containing 3 nmol phosphate for a PEI/DNA ratio equal to a nitrogen/phosphate ratio of 7 for transfection; (3) Ultrasound + plasmid group (US + P), plasmid 15 µg; (4) Ultrasound + cationic liposomal ultrasound contrast agent + plasmid group (UTMD + P group), plasmid 15 µg and cationic liposomal ultrasound contrast agent 5%; (5) Ultrasound + cationic liposomal ultrasound contrast agent + PEI + plasmid group (UTMD + PEI + P group), The influence on Hela cells was observed under microscope, the efficiency of apoptosis was measured by flow cytometry, and cell viability was tested in CCK 8. Results: The optimized transfection parameters can improve the transfection efficiency of ultrasound combined with CUCA to a certain extent, but its transfection efficiency is still lower than that of branched polyethyleneimine (bPEI) 25 kDa. By investigating the effect of HeLa cells apoptosis induced by UTMD in combination with PEI mediated survivin miRNA, we found that both PEI alone and ultrasound in combination with C-UCA were able to transfect cells with survivin miRNA to effectively induce HeLa cell apoptosis. However, the synergistic effect between the two methods was not significant. Conclusion: In contrast, the combined use of ultrasound, C-UCA and PEI may significantly reduce the transfection efficiency of UTMD and PEI, and the specific mechanism remains to be further studied.

Lack of an Immune Response to Albunex®, a New Ultrasound Contrast Agent Based on Air-Filled Albumin Microspheres

1994 ◽  
Vol 104 (4) ◽  
pp. 372-378 ◽  
Author(s):  
Cathrine Christiansen ◽  
Anne Juelsrud Vebner ◽  
Berit Muan ◽  
Hogne Vik ◽  
Trond Haider ◽  
...  
Keyword(s):  
Immune Response ◽  
Contrast Agent ◽  
Ultrasound Contrast Agent ◽  
Agent Based ◽  
Albumin Microspheres ◽  
Ultrasound Contrast

Novel ultrasound contrast agent based on microbubbles generated from surfactant mixtures of Span 60 and polyoxyethylene 40 stearate

2010 ◽  
Vol 6 (9) ◽  
pp. 3542-3549 ◽  
Author(s):  
Zhanwen Xing ◽  
Hengte Ke ◽  
Jinrui Wang ◽  
Bo Zhao ◽  
Xiuli Yue ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Ultrasound Contrast Agent ◽  
Surfactant Mixtures ◽  
Agent Based ◽  
Ultrasound Contrast ◽  
Span 60

scholarly journals Transdermal Drug Delivery Aided by an Ultrasound Contrast Agent: An In Vitro Experimental Study

2010 ◽  
Vol 4 (1) ◽  
pp. 56-62 ◽  
Author(s):  
Donghee Park ◽  
Jinhee Yoon ◽  
Jingam Park ◽  
Byungjo Jung ◽  
Hyunjin Park ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Contrast Agent ◽  
Transdermal Drug Delivery ◽  
Ultrasound Contrast Agent ◽  
Low Frequency ◽  
Optical Measurements ◽  
Skin Permeability ◽  
Target Drug ◽  
Ultrasound Contrast

Sonophoresis temporarily increases skin permeability such that medicine can be delivered transdermally. Cavitation is believed to be the predominant mechanism in sonophoresis. In this study, an ultrasound contrast agent (UCA) strategy was adopted instead of low frequency ultrasound to assure that cavitation occurred, and the efficacy of sonophoresis with UCA was quantitatively analyzed by optical measurements. The target drug used in this study was 0.1 % Definity® in 70% glycerol, which was delivered into porcine skin samples. Glycerol was used because it is an optical clearing agent, and the efficiency of glycerol delivery could be analyzed with optical measurements. The applied acoustic pressure was approximately 600 kPa at 1 MHz ultrasound with a 10% duty cycle for 60 minutes. Experimental results indicated that the measured relative contrast (RC) after sonophoresis with UCA was approximately 80% higher than RC after sonophoresis without UCA. In addition, the variance of RC was also reduced by more than 50% with the addition of a UCA. The use of a UCA appeared to increase cavitation, demonstrating that the use of a UCA can be effective in transdermal drug delivery (TDD).

A New Ultrasound Contrast Agent Based on Biodegradable Polymeric Microballoons

1991 ◽  
Vol 26 ◽  
pp. S190-S191 ◽  
Author(s):  
M. SCHNEIDER ◽  
P. BUSSAT ◽  
M. B. BARRAU ◽  
F. BODINO ◽  
C. GOTTI ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Ultrasound Contrast Agent ◽  
Agent Based ◽  
Ultrasound Contrast

Balancing stealth and echogenic properties in an ultrasound contrast agent with drug delivery potential

Biomaterials ◽  
2016 ◽  
Vol 103 ◽  
pp. 197-206 ◽  
Author(s):  
Lauren J. Jablonowski ◽  
David Alfego ◽  
James I. Andorko ◽  
John R. Eisenbrey ◽  
Nutte Teraphongphom ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Contrast Agent ◽  
Ultrasound Contrast Agent ◽  
Ultrasound Contrast

Low-frequency and high-intensity ultrasonic may play a better role in Ultrasound Contrast Agent drug delivery system in vivo

2009 ◽  
Vol 73 (2) ◽  
pp. 211-212 ◽  
Author(s):  
Jia-an Zhu ◽  
Yi-zhou Hu ◽  
Ye-qing Jiang ◽  
Chun-yan Xing ◽  
Dian-cheng Li ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Contrast Agent ◽  
Drug Delivery System ◽  
Delivery System ◽  
High Intensity ◽  
Ultrasound Contrast Agent ◽  
Low Frequency ◽  
Ultrasound Contrast
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