scholarly journals Class I Polyhydroxyalkanoate Synthase from the Purple Photosynthetic Bacterium Rhodovulum sulfidophilum Predominantly Exists as a Functional Dimer in the Absence of a Substrate

ACS Omega ◽  
2017 ◽  
Vol 2 (8) ◽  
pp. 5071-5078 ◽  
Author(s):  
Mieko Higuchi-Takeuchi ◽  
Yoko Motoda ◽  
Takanori Kigawa ◽  
Keiji Numata
Keyword(s):  
Photosynthetic Bacterium ◽  
Class I ◽  
Rhodovulum Sulfidophilum ◽  
Polyhydroxyalkanoate Synthase ◽  
Purple Photosynthetic Bacterium

Reduction of the oxidized bacteriochlorophyll dimer in reaction centers by ferrocene is dependent upon the driving force

2007 ◽  
Vol 11 (03) ◽  
pp. 205-211 ◽  
Author(s):  
László Kálmán ◽  
Arlene L. M. Haffa ◽  
JoAnn C. Williams ◽  
Neal W. Woodbury ◽  
James P. Allen
Keyword(s):  
Electron Transfer ◽  
Rate Constants ◽  
Ph Dependence ◽  
Photosynthetic Bacterium ◽  
Energy Difference ◽  
Reaction Centers ◽  
Free Energy Difference ◽  
Midpoint Potential ◽  
Bacteriochlorophyll Dimer ◽  
Purple Photosynthetic Bacterium

The rates of electron transfer from ferrocene to the oxidized bacteriochlorophyll dimer, P , in reaction centers from the purple photosynthetic bacterium Rhodobacter sphaeroides, were measured for a series of mutants in which the P / P + midpoint potentials range from 410 to 765 mV (Lin et al. Proc. Natl. Acad. Sci. USA 1994; 91: 10265-10269). The observed rate constant for each mutant was found to be linearly dependent upon the ferrocene concentration up to 50 μM. The electron transfer is described as a second order reaction with rate constants increasing from 1.5 to 35 × 106 M -1. s -1 with increasing P / P + midpoint potential. This dependence was tested for three additional mutants, each of which exhibits a pH dependence of the P / P + midpoint potential due to an electrostatic interaction with an introduced carboxylic group (Williams et al. Biochemistry 2001; 40: 15403-15407). For these mutants, the pH dependence of the bimolecular rate constants followed a sigmoidal pattern that could be described with a Henderson-Hasselbalch equation, attributable to the change of the free energy difference for the reaction due to deprotonation of the introduced carboxylic side chains.

Characterization of extracellular DNA production and flocculation of the marine photosynthetic bacterium Rhodovulum sulfidophilum

2009 ◽  
Vol 84 (2) ◽  
pp. 349-356 ◽  
Author(s):  
Hiromichi Suzuki ◽  
Masahide Daimon ◽  
Tomoyuki Awano ◽  
So Umekage ◽  
Terumichi Tanaka ◽  
...  
Keyword(s):  
Photosynthetic Bacterium ◽  
Extracellular Dna ◽  
Rhodovulum Sulfidophilum ◽  
Dna Production ◽  
Marine Photosynthetic Bacterium
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