scholarly journals Synthetic Matrices for Intestinal Organoid Culture: Implications for Better Performance

ACS Omega ◽  
2021 ◽  
Author(s):  
Humendra Poudel ◽  
Karie Sanford ◽  
Peter K. Szwedo ◽  
Rupak Pathak ◽  
Anindya Ghosh
Keyword(s):  
Organoid Culture ◽  
Synthetic Matrices

scholarly journals Niche-inspired synthetic matrices for epithelial organoid culture

2019 ◽  
Author(s):  
Victor Hernandez-Gordillo ◽  
Timothy Kassis ◽  
Arinola Lampejo ◽  
GiHun Choi ◽  
Mario E. Gamboa ◽  
...  
Keyword(s):  
Single Cells ◽  
Biophysical Properties ◽  
Organoid Culture ◽  
Apicobasal Polarity ◽  
Induction Of Differentiation ◽  
Synthetic Matrix ◽  
Synthetic Matrices ◽  
Α2β1 Integrin ◽  
Dominant Parameter ◽  
Human Implantation

AbstractEpithelial organoids are now an important tool in fields ranging from regenerative medicine to drug discovery. Organoid culture requires Matrigel, a complex, tumor-derived, extracellular matrix. An alternative completely synthetic matrix could improve culture reproducibility, clarify mechanistic phenomena, and enable applications involving human implantation. Here, we designed synthetic matrices with tunable biomolecular and biophysical properties that allowed us to identify critical gel parameters in organoid formation. Inspired by known epithelial integrin expression in the proliferative niche of the human intestine, we identified an α2β1 integrin-binding peptide as a critical component of the synthetic matrix that supports human duodenal colon and endometrial organoid propagation. We show that organoids emerge from single cells, retain their proliferative capacity, are functionally responsive to basolateral stimulation and have correct apicobasal polarity upon induction of differentiation. The local biophysical presentation of the cues, rather than bulk mechanical properties, appears to be the dominant parameter governing epithelial cell proliferation and organoid formation in the synthetic matrix.

Whole exome sequencing and establishment of an organoid culture of the carcinoma showing thymus-like differentiation (CASTLE) of the parotid gland

2021 ◽  
Author(s):  
Tomohiko Ishikawa ◽  
Takenori Ogawa ◽  
Ayako Nakanome ◽  
Yasunari Yamauchi ◽  
Hajime Usubuchi ◽  
...  
Keyword(s):  
Parotid Gland ◽  
Exome Sequencing ◽  
Whole Exome Sequencing ◽  
Organoid Culture ◽  
Whole Exome

scholarly journals Quantification of Cardiomyocyte Beating Frequency Using Fourier Transform Analysis

Photonics ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 39 ◽  
Author(s):  
Allison Reno ◽  
Andrew Hunter ◽  
Yang Li ◽  
Tong Ye ◽  
Ann Foley
Keyword(s):  
Fourier Transform ◽  
Fast Fourier Transform ◽  
Sinoatrial Node ◽  
Frequency Data ◽  
True Rate ◽  
Video Clips ◽  
Organoid Culture ◽  
Cholinergic Signaling ◽  
Beating Frequency

Pacemaker cardiomyocytes of the sinoatrial node (SAN) beat more rapidly than cells of the working myocardium. Beating in SAN cells responds to β-adrenergic and cholinergic signaling by speeding up or slowing, respectively. Beat rate has traditionally been assessed using voltage or calcium sensitive dyes, however these may not reflect the true rate of beating because they sequester calcium. Finally, in vitro differentiated cardiomyocytes sometimes briefly pause during imaging giving inaccurate beat rates. We have developed a MATLAB automation to calculate cardiac beat rates directly from video clips based on changes in pixel density at the edges of beating areas. These data are normalized to minimize the effects of secondary movement and are converted to frequency data using a fast Fourier transform (FFT). We find that this gives accurate beat rates even when there are brief pauses in beating. This technique can be used to rapidly assess beating of cardiomyocytes in organoid culture. This technique could also be combined with field scanning techniques to automatically and accurately assess beating within a complex cardiac organoid.

scholarly journals Crypt Organoid Culture as an In Vitro Model in Drug Metabolism and Cytotoxicity Studies

2017 ◽  
Vol 45 (7) ◽  
pp. 748-754 ◽  
Author(s):  
Wenqi Lu ◽  
Eva Rettenmeier ◽  
Miles Paszek ◽  
Mei-Fei Yueh ◽  
Robert H. Tukey ◽  
...  
Keyword(s):  
Drug Metabolism ◽  
In Vitro Model ◽  
Organoid Culture ◽  
Cytotoxicity Studies ◽  
Vitro Model

Current status of organoid culture in urological malignancy

2021 ◽  
Author(s):  
Ryan Tsz‐Hei Tse ◽  
Hongda Zhao ◽  
Christine Yim‐Ping Wong ◽  
Peter Ka‐Fung Chiu ◽  
Jeremy Yuen‐Chun Teoh ◽  
...  
Keyword(s):  
Current Status ◽  
Organoid Culture

scholarly journals Protein-functionalized poly(ethylene glycol) hydrogels as scaffolds for monolayer organoid culture

2020 ◽  
Author(s):  
Reid L Wilson ◽  
Ganesh Swaminathan ◽  
Khalil Ettayebi ◽  
Carolyn Bomidi ◽  
Xi-Lei Zeng ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Organoid Culture ◽  
Poly Ethylene
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