scholarly journals Immobilization of Lipase from Thermomyces lanuginosus in Magnetic Macroporous ZIF-8 Improves Lipase Reusability in Biodiesel Preparation

ACS Omega ◽  
2021 ◽  
Author(s):  
Yuhan Li ◽  
Hao Zhou ◽  
Lingmei Dai ◽  
Dehua Liu ◽  
Sulaiman Al-Zuhair ◽  
...  
Keyword(s):  
Thermomyces Lanuginosus

Amylase hyperproduction by deregulated mutants of the thermophilic fungus Thermomyces lanuginosus

2002 ◽  
Vol 29 (2) ◽  
pp. 70-74
Author(s):  
K Rubinder ◽  
BS Chadha ◽  
N Singh ◽  
HS Saini ◽  
S Singh
Keyword(s):  
Thermophilic Fungus ◽  
Thermomyces Lanuginosus

scholarly journals Design of a New Gemini Lipoaminoacid with Immobilized Lipases Based on an Eco-Friendly Biosynthetic Process

Catalysts ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 164
Author(s):  
Patrícia M. Carvalho ◽  
Rita C. Guedes ◽  
Maria R. Bronze ◽  
Célia M. C. Faustino ◽  
Maria H. L. Ribeiro
Keyword(s):  
High Performance ◽  
Transfection Efficiency ◽  
Sol Gel ◽  
Reaction Rates ◽  
Solvent Free ◽  
Bioactive Molecules ◽  
Thermomyces Lanuginosus ◽  
Chemical Production ◽  
Immobilized Lipases ◽  
Free Media

Lipoaminoacids (LAA) are an important group of biosurfactants, formed by a polar hydrophilic part (amino acid) and a hydrophobic tail (lipid). The gemini LAA structures allow the formation of a supramolecular complex with bioactive molecules, like DNA, which provides them with good transfection efficiency. Since lipases are naturally involved in lipid and protein metabolism, they are an alternative to the chemical production of LAA, offering an eco-friendly biosynthetic process option. This work aimed to design the production of novel cystine derived gemini through a bioconversion system using immobilized lipases. Three lipases were used: porcine pancreatic lipase (PPL); lipase from Thermomyces lanuginosus (TLL); and lipase from Rizhomucor miehei (RML). PPL was immobilized in sol-gel lenses. L-cystine dihydrochloride and dodecylamine were used as substrates for the bioreaction. The production of LAA was evaluated by thin layer chromatography (TLC), and colorimetric reaction with eosin. The identification and quantification was carried out by High Performance Liquid Chromatographer-Mass Spectrometry (HPLC-MS/MS). The optimization of media design included co-solvent (methanol, dimethylsulfoxide), biphasic (n-hexane and 2-propanol) or solvent-free media, in order to improve the biocatalytic reaction rates and yields. Moreover, a new medium was tested where dodecylamine was melted and added to the cystine and to the biocatalyst, building a system of mainly undissolved substrates, leading to 5 mg/mL of LAA. Most of the volume turned into foam, which indicated the production of the biosurfactant. For the first time, the gemini derived cystine lipoaminoacid was produced, identified, and quantified in both co-solvent and solvent-free media, with the lipases PPL, RML, and TLL.

Comparative biocontrol ability of chitinases from bacteria and recombinant chitinases from the thermophilic fungus Thermomyces lanuginosus

2019 ◽  
Vol 127 (6) ◽  
pp. 663-671 ◽  
Author(s):  
Ruth Nyanduko Okongo ◽  
Adarsh Kumar Puri ◽  
Zhengxiang Wang ◽  
Suren Singh ◽  
Kugen Permaul
Keyword(s):  
Thermophilic Fungus ◽  
Thermomyces Lanuginosus

Promoting protein self-association in non-glycosylated Thermomyces lanuginosus lipase based on crystal lattice contacts

2015 ◽  
Vol 1854 (12) ◽  
pp. 1914-1921 ◽  
Author(s):  
Jens Kvist Madsen ◽  
Thomas Rebsdorf Sørensen ◽  
Jørn Døvling Kaspersen ◽  
Maria Berggård Silow ◽  
Jesper Vind ◽  
...  
Keyword(s):  
Crystal Lattice ◽  
Thermomyces Lanuginosus ◽  
Thermomyces Lanuginosus Lipase ◽  
Self Association

scholarly journals Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145

2002 ◽  
Vol 33 (4) ◽  
Author(s):  
Mônica Caramez Triches Damaso ◽  
Carolina M.M. Carvalho Andrade ◽  
Nei Pereira Jr
Keyword(s):  
Thermomyces Lanuginosus

scholarly journals Production of Structured Phosphatidylcholine with High Content of Myristic Acid by Lipase-Catalyzed Acidolysis and Interesterification

Catalysts ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 281 ◽  
Author(s):  
Anna Chojnacka ◽  
Witold Gładkowski
Keyword(s):  
Myristic Acid ◽  
Rhizomucor Miehei ◽  
Egg Yolk ◽  
Thermomyces Lanuginosus ◽  
Molar Ratio ◽  
Acyl Donor ◽  
Thermomyces Lanuginosus Lipase ◽  
Screening Experiments ◽  
Miehei Lipase ◽  
Acyl Donors

Synthesis of structured phosphatidylcholine (PC) enriched with myristic acid (MA) was conducted by acidolysis and interesterification reactions using immobilized lipases as catalysts and two acyl donors: trimyristin (TMA) isolated from ground nutmeg, and myristic acid obtained by saponification of TMA. Screening experiments indicated that the most effective biocatalyst for interesterification was Rhizomucor miehei lipase (RML), whereas for acidolysis, the most active were Thermomyces lanuginosus lipase (TLL) and RML. The effect of the molar ratio of substrates (egg-yolk PC/acyl donor), enzyme loading, and different solvent on the incorporation of MA into PC and on PC recovery was studied. The maximal incorporation of MA (44 wt%) was achieved after 48 h of RML-catalyzed interesterification in hexane using substrates molar ratio (PC/trimyristin) 1/5 and 30% enzyme load. Comparable results were obtained in toluene with 1/3 substrates molar ratio. Interesterification of PC with trimyristin resulted in significantly higher MA incorporation than acidolysis with myristic acid, particularly in the reactions catalyzed by RML.

Enzymatic synthesis of a chiral chalcogran intermediate

2014 ◽  
Vol 68 (6) ◽  
Author(s):  
Vladimír Mastihuba ◽  
Pavel Čepec ◽  
Silvia Vlčková ◽  
Erika Farkašová ◽  
Mária Mastihubová ◽  
...  
Keyword(s):  
Vinyl Acetate ◽  
Bark Beetle ◽  
Enzymatic Synthesis ◽  
Kinetic Resolution ◽  
Thermomyces Lanuginosus ◽  
Methyl Tert Butyl Ether ◽  
Lipozyme Tl Im ◽  
Butyl Ether ◽  
Novozyme 435 ◽  
Reaction Media

AbstractTwo lipases, Novozyme 435 (lipase B from Candida Antarctica) and Lipozyme TL IM (Thermomyces lanuginosus) were used successfully for the kinetic resolution of racemic 1-(2-furyl)-3-pentanol, the key intermediate in synthesis of the bark beetle pheromone, chalcogran. The desired S-(+)-enantiomer was prepared in enantiomeric excesses higher than 98 % and with yields of 26.3 % and 32.5 %, respectively. Methyl tert-butyl ether and vinyl acetate were found to be the best reaction media and the acetyl donor to achieve fast and effective resolution.

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