scholarly journals Structural Characterization of the Human Cytosolic Malate Dehydrogenase I

ACS Omega ◽  
2021 ◽  
Author(s):  
William M. McCue ◽  
Barry C. Finzel
Keyword(s):  
Malate Dehydrogenase ◽  
Structural Characterization ◽  
Cytosolic Malate Dehydrogenase

scholarly journals Cloning, expression and biochemical characterization of mitochondrial and cytosolic malate dehydrogenase from Phytophthora infestans

2009 ◽  
Vol 113 (6-7) ◽  
pp. 771-781 ◽  
Author(s):  
Patricia E. López-Calcagno ◽  
Johanna Moreno ◽  
Luis Cedeño ◽  
Luis Labrador ◽  
Juan L. Concepción ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Malate Dehydrogenase ◽  
Biochemical Characterization ◽  
Cytosolic Malate Dehydrogenase

Cloning, sequencing and functional expression of a DNA encoding pig cytosolic malate dehydrogenase: purification and characterization of the recombinant enzyme

Gene ◽  
1996 ◽  
Vol 172 (2) ◽  
pp. 303-308 ◽  
Author(s):  
Francisca Trejo ◽  
Montserrat Costa ◽  
Josep Lluis Gelpi ◽  
Montserrat Busquets ◽  
Anthony R. Clarke ◽  
...  
Keyword(s):  
Malate Dehydrogenase ◽  
Functional Expression ◽  
Recombinant Enzyme ◽  
Dna Encoding ◽  
Purification And Characterization ◽  
Cytosolic Malate Dehydrogenase

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

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