scholarly journals Further Studies on Cationic Gemini Amphiphiles as Carriers for Gene Delivery─The Effect of Linkers in the Structure and Other Factors Affecting the Transfection Efficacy of These Amphiphiles

ACS Omega ◽  
2021 ◽  
Author(s):  
Mange Ram Yadav ◽  
Mukesh Kumar ◽  
Prashant R. Murumkar
Keyword(s):  
Gene Delivery ◽  
Factors Affecting ◽  
Transfection Efficacy ◽  
Gemini Amphiphiles

Nanoparticles Composed by Oligochitosan and Polyethylenimine for Gene Delivery

2013 ◽  
Vol 284-287 ◽  
pp. 418-422
Author(s):  
Chi Hsien Liu ◽  
Mei Shan Cheng
Keyword(s):  
Gene Delivery ◽  
Cell Lines ◽  
Fluorescent Protein ◽  
Synergistic Effects ◽  
Image Analyzer ◽  
Transfection Efficacy ◽  
Fluorescence Protein ◽  
Green Fluorescent ◽  
3T3 Cell Lines

Cationic carriers including polyethylenimine, liposomes, and chitosan have been used to transfer plasmid DNA in vitro by condensing anionic DNA. Here, oligochitosan (OC) was found to have capacity for in-vitro gene delivery in four cell lines. Plasmid containing green fluorescent protein (GFP) gene was used as a reporter gene. The transfection efficacy and cell viability of the transfection vehicles were analyzed by using a high-throughput image analyzer. We found that DNA polyplexes formed by high dosage of OC could be efficiently delivered into the cells. The combination of OC and polyethylenimine (PEI) were found to significantly enhance the fluorescence protein expression. The introduction of oligochitosan in PEI-mediated transfection could increase the transfection efficacy and could reduce the toxicity of PEI. Additionally, the synergistic effects of PEI and OC were confirmed in CHO, Caco2, Hep-SK, and 3T3 cell lines. The detailed mechanism of PEI and oligochitosan on transfection was investigated by using gel retardation and DNase degradation experiments. A facile and inexpensive construction of gene delivering vehicles was developed herein by using oligochitosan and PEI.

Gemini Amphiphiles: A Novel Class of Nonviral Gene Delivery Vectors

2010 ◽  
Vol 27 (3) ◽  
pp. 237-278 ◽  
Author(s):  
Mukesh Kumar ◽  
Kaustubh Jinturkar ◽  
M.R. Yadav ◽  
Ambikanandan Misra
Keyword(s):  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Gene Delivery Vectors ◽  
Gemini Amphiphiles

A core–shell structured polyplex for efficient and non-toxic gene delivery

2017 ◽  
Vol 5 (26) ◽  
pp. 5101-5108 ◽  
Author(s):  
Saisai Wang ◽  
Fei Wang ◽  
Qiang Zhang ◽  
Yiyun Cheng
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Core Shell ◽  
Low Molecular Weight ◽  
The Core ◽  
Transfection Efficacy ◽  
Toxic Gene ◽  
Low Toxicity ◽  
Linear Polyethylenimine

We developed a core–shell polyplex with minimal high generation dendrimer to condense DNA and low-molecular-weight linear polyethylenimine coated on the core. The polyplex represented both high transfection efficacy and low toxicity.

scholarly journals Physical Factors Affecting Plasmid DNA Compaction in Stearylamine-Containing Nanoemulsions Intended for Gene Delivery

2012 ◽  
Vol 5 (6) ◽  
pp. 643-654 ◽  
Author(s):  
André Leandro Silva ◽  
Francisco Alexandrino ◽  
Lourena Mafra Verissimo ◽  
Lucymara Fassarella Agnez-Lima ◽  
Lucila Carmem Monte Egito ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
Physical Factors ◽  
Factors Affecting ◽  
Dna Compaction
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