scholarly journals Electrochromogenic Detection of Live Bacteria Using Soluble and Insoluble Prussian Blue

ACS Omega ◽  
2021 ◽  
Author(s):  
Amparo Ferrer-Vilanova ◽  
Yasmine Alonso ◽  
Josune J Ezenarro ◽  
Sara Santiago ◽  
Xavier Muñoz-Berbel ◽  
...  
Keyword(s):  
Prussian Blue ◽  
Live Bacteria

Bacterial Peptidoglycan Stapling with Functionalized D-Amino Acids

2019 ◽  
Author(s):  
Sylvia L. Rivera ◽  
Akbar Espaillat ◽  
Arjun K. Aditham ◽  
Peyton Shieh ◽  
Chris Muriel-Mundo ◽  
...  
Keyword(s):  
Cell Wall ◽  
Clinical Success ◽  
Bacterial Species ◽  
Enzymatic Reaction ◽  
Amino Acid Derivative ◽  
Wall Structure ◽  
Live Bacteria ◽  
Cross Links ◽  
Osmotic Lysis ◽  
Bacterial Peptidoglycan

Transpeptidation reinforces the structure of cell wall peptidoglycan, an extracellular heteropolymer that protects bacteria from osmotic lysis. The clinical success of transpeptidase-inhibiting β-lactam antibiotics illustrates the essentiality of these cross-linkages for cell wall integrity, but the presence of multiple, seemingly redundant transpeptidases in many bacterial species makes it challenging to determine cross-link function precisely. Here we present a technique to covalently link peptide strands by chemical rather than enzymatic reaction. We employ bio-compatible click chemistry to induce triazole formation between azido- and alkynyl-D-alanine residues that are metabolically installed in the cell walls of Gram-positive and Gram-negative bacteria. Synthetic triazole cross-links can be visualized by substituting azido-D-alanine with azidocoumarin-D-alanine, an amino acid derivative that undergoes fluorescent enhancement upon reaction with terminal alkynes. Cell wall stapling protects the model bacterium Escherichia coli from β-lactam treatment. Chemical control of cell wall structure in live bacteria can provide functional insights that are orthogonal to those obtained by genetics.<br>

Controlled synthesis of functional Ag, Ag–Au/Au–Ag nanoparticles and their Prussian blue nanocomposites for bioanalytical applications

RSC Advances ◽  
2015 ◽  
Vol 5 (61) ◽  
pp. 49671-49679 ◽  
Author(s):  
Prem. C. Pandey ◽  
Richa Singh ◽  
Yashashwa Pandey
Keyword(s):  
Prussian Blue ◽  
Ag Nanoparticles ◽  
Active Role ◽  
Reducing Agents ◽  
Controlled Synthesis ◽  
Bioanalytical Applications

A facile method for the synthesis of functional AgNPs and bimetallic Ag–Au/Au–Ag are reported, enabling the formation of nanocomposite with prussian blue in a crystalline framework for bioanalytical applications, showing the active role of organic reducing agents and 3-aminopropyltrimethoxysilane.

Sign in / Sign up

Export Citation Format

Share Document