scholarly journals CpACpP: In Silico Cell-Penetrating Anticancer Peptide Prediction Using a Novel Bioinformatics Framework

ACS Omega ◽  
2021 ◽  
Author(s):  
Farid Nasiri ◽  
Fereshteh Fallah Atanaki ◽  
Saman Behrouzi ◽  
Kaveh Kavousi ◽  
Mojtaba Bagheri
Keyword(s):  
In Silico ◽  
Anticancer Peptide ◽  
Cell Penetrating ◽  
Peptide Prediction

scholarly journals In Silico Bioactive Peptide Prediction from The Enzymatic Hydrolysates of Edible Seaweed Rubisco Large Chain

2021 ◽  
Vol 21 (12) ◽  
pp. 615-625
Author(s):  
Ayse Kose
Keyword(s):  
In Silico ◽  
Bioactive Peptides ◽  
Antioxidant Properties ◽  
In Silico Analysis ◽  
Bioactive Peptide ◽  
Bioactive Substances ◽  
Edible Seaweed ◽  
Ic50 Values ◽  
Peptide Prediction ◽  
Ancient Food

Seaweeds are one of the ancient food supplements on Earth. Especially Asian countries use seaweeds as the fundamental ingredient in their cuisine. Seaweeds are photosynthetic organisms living in aquatic ecosystems and in the coastal territories. Seaweeds out of farm areas are frequently observed as coastal wastes. However, seaweeds are outstanding sources for bioactive substances and investigation bioactive properties of seaweed RuBisCO has never been done. RuBisCO is the most abundant protein on Earth but a vast amount of RuBisCO goes through waste. In this study, bioactive peptide prediction of frequently consumed seaweed RuBisCO proteins were analyzed in silico to identify possible bioactive peptides as substitute or support for grain, meat, and dairy based bioactive peptides. A huge portion of peptides were di-, tri- peptides with IC50 values less than 300 µM according to the comparison of BIOPEP database. Including gastric digestion, more than half of the peptides showed DDP-IV and ACE inhibitory activity followed by antioxidant properties. Also, novel antiinflammatory and anti-cancer peptides were found through in silico analysis.

In silico peptide prediction for antibody generation to recognize 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in genetically modified organisms

Biopolymers ◽  
2015 ◽  
Vol 104 (2) ◽  
pp. 91-100 ◽  
Author(s):  
Mariela M. Marani ◽  
Joana Costa ◽  
Isabel Mafra ◽  
Maria Beatriz P.P. Oliveira ◽  
Silvia A. Camperi ◽  
...  
Keyword(s):  
In Silico ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Peptide Prediction ◽  
Phosphate Synthase

scholarly journals In silico approaches for designing highly effective cell penetrating peptides

2013 ◽  
Vol 11 (1) ◽  
pp. 74 ◽  
Author(s):  
Ankur Gautam ◽  
Kumardeep Chaudhary ◽  
Rahul Kumar ◽  
Arun Sharma ◽  
Pallavi Kapoor ◽  
...  
Keyword(s):  
In Silico ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating ◽  
Highly Effective

Discovering New In Silico Tools for Antimicrobial Peptide Prediction

2012 ◽  
Vol 13 (9) ◽  
pp. 1148-1157 ◽  
Author(s):  
Marc Torrent ◽  
M. Victoria Nogues ◽  
Ester Boix
Keyword(s):  
Antimicrobial Peptide ◽  
In Silico ◽  
Peptide Prediction ◽  
In Silico Tools

scholarly journals In Silico Studies of Asymmetric Membranes Perturbations Caused by Dynamic Aggregation of a Cell-Penetrating Peptide

2014 ◽  
Vol 106 (2) ◽  
pp. 452a
Author(s):  
Jean Helie ◽  
Mickael Lelimousin ◽  
Charlotte M. Deane ◽  
Francesca Milletti ◽  
Mark S.P. Sansom
Keyword(s):  
In Silico ◽  
Cell Penetrating Peptide ◽  
Asymmetric Membranes ◽  
In Silico Studies ◽  
Cell Penetrating ◽  
A Cell

Human-protein-derived peptides for intracellular delivery of biomolecules

2012 ◽  
Vol 442 (3) ◽  
pp. 583-593 ◽  
Author(s):  
Alexander K. Haas ◽  
Daniela Maisel ◽  
Juliane Adelmann ◽  
Christoffer von Schwerin ◽  
Ines Kahnt ◽  
...  
Keyword(s):  
Cell Viability ◽  
In Silico ◽  
Cultured Cells ◽  
Cell Penetrating Peptides ◽  
Extracellular Proteins ◽  
Human Protein ◽  
Rna Transfection ◽  
Cell Penetrating ◽  
High Concentrations ◽  
Α Helix

Access of therapeutic biomolecules to cytoplasmic and nuclear targets is hampered by the inability of these molecules to cross biological membranes. Approaches to overcome this hurdle involve CPPs (cell-penetrating peptides) or protein transduction domains. Most of these require rather high concentrations to elicit cell-penetrating functionality, are non-human, pathogen-derived or synthetic entities, and may therefore not be tolerated or even immunogenic. We identified novel human-protein-derived CPPs by a combination of in silico and experimental analyses: polycationic CPP candidates were identified in an in silico library of all 30-mer peptides of the human proteome. Of these peptides, 60 derived from extracellular proteins were evaluated experimentally. Cell viability and siRNA (small interfering RNA) transfection assays revealed that 20 out of the 60 peptides were functional. Three of these showed CPP functionality without interfering with cell viability. A peptide derived from human NRTN (neurturin), which contains an α-helix, performed the best in our screen and was uniformly taken up by cultured cells. Examples for payloads that can be delivered to the cytosol by the NRTN peptide include complexed siRNAs and both N- and C-terminally fused pro-apoptotic peptides.

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