scholarly journals Mycobacterium tuberculosis Cell Wall Permeability Model Generation Using Chemoinformatics and Machine Learning Approaches

ACS Omega ◽  
2021 ◽  
Author(s):  
Selvaraman Nagamani ◽  
G. Narahari Sastry
Keyword(s):  
Machine Learning ◽  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
Model Generation ◽  
Learning Approaches ◽  
Permeability Model ◽  
Wall Permeability ◽  
Cell Wall Permeability

scholarly journals 3D-QSAR and Cell Wall Permeability of Antitubercular Nitroimidazoles against Mycobacterium tuberculosis

Molecules ◽  
2013 ◽  
Vol 18 (11) ◽  
pp. 13870-13885 ◽  
Author(s):  
Sang-Ho Lee ◽  
Minsung Choi ◽  
Pilho Kim ◽  
Pyung Myung
Keyword(s):  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
3D Qsar ◽  
Wall Permeability ◽  
Cell Wall Permeability

scholarly journals Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis

2021 ◽  
Vol 12 ◽  
Author(s):  
Cheng-Cheung Chen ◽  
Yih-Yuan Chen ◽  
Chang-Ching Yeh ◽  
Chia-Wei Hsu ◽  
Shang-Jie Yu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
Therapeutic Option ◽  
Infectious Agent ◽  
Drug Susceptibility ◽  
Drug Resistant ◽  
Wall Permeability ◽  
Cell Wall Permeability

Tuberculosis (TB) is a leading cause of death from a single infectious agent, Mycobacterium tuberculosis (Mtb). Although progress has been made in TB control, still about 10 million people worldwide develop TB annually and 1.5 million die of the disease. The rapid emergence of aggressive, drug-resistant strains and latent infections have caused TB to remain a global health challenge. TB treatments are lengthy and their side effects lead to poor patient compliance, which in turn has contributed to the drug resistance and exacerbated the TB epidemic. The relatively low output of newly approved antibiotics has spurred research interest toward alternative antibacterial molecules such as silver nanoparticles (AgNPs). In the present study, we use the natural biopolymer alginate to serve as a stabilizer and/or reductant to green synthesize AgNPs, which improves their biocompatibility and avoids the use of toxic chemicals. The average size of the alginate-capped AgNPs (ALG-AgNPs) was characterized as nanoscale, and the particles were round in shape. Drug susceptibility tests showed that these ALG-AgNPs are effective against both drug-resistant Mtb strains and dormant Mtb. A bacterial cell-wall permeability assay showed that the anti-mycobacterial action of ALG-AgNPs is mediated through an increase in cell-wall permeability. Notably, the anti-mycobacterial potential of ALG-AgNPs was effective in both zebrafish and mouse TB animal models in vivo. These results suggest that ALG-AgNPs could provide a new therapeutic option to overcome the difficulties of current TB treatments.

scholarly journals Deficiency of the Novel Exopolyphosphatase Rv1026/PPX2 Leads to Metabolic Downshift and Altered Cell Wall Permeability in Mycobacterium tuberculosis

mBio ◽  
2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Yu-Min Chuang ◽  
Nirmalya Bandyopadhyay ◽  
Dalin Rifat ◽  
Harvey Rubin ◽  
Joel S. Bader ◽  
...  
Keyword(s):  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
Stringent Response ◽  
Inorganic Polyphosphate ◽  
Content Type ◽  
P Accumulation ◽  
Wall Permeability ◽  
Altered Cell ◽  
Cell Wall Permeability ◽  
Long Chain

ABSTRACTMycobacterium tuberculosiscan persist for decades in the human host. Stringent response pathways involving inorganic polyphosphate [poly(P)], which is synthesized and hydrolyzed by polyphosphate kinase (PPK) and exopolyphosphatase (PPX), respectively, are believed to play a key regulatory role in bacterial persistence. We show here thatM. tuberculosispoly(P) accumulation is temporally linked to bacillary growth restriction. We also identifyM. tuberculosisRv1026 as a novel exopolyphosphatase with hydrolytic activity against long-chain poly(P). Using a tetracycline-inducible expression system to knock down expression ofRv1026(ppx2), we found thatM. tuberculosispoly(P) accumulation leads to slowed growth and reduced susceptibility to isoniazid, increased resistance to heat and acid pH, and enhanced intracellular survival during macrophage infection. By transmission electron microscopy, theppx2knockdown strain exhibited increased cell wall thickness, which was associated with reduced cell wall permeability to hydrophilic drugs rather than induction of drug efflux pumps or altered biofilm formation relative to the empty vector control. Transcriptomic and metabolomic analysis revealed a metabolic downshift of theppx2knockdown characterized by reduced transcription and translation and a downshift of glycerol-3-phosphate levels. In summary, poly(P) plays an important role inM. tuberculosisgrowth restriction and metabolic downshift and contributes to antibiotic tolerance through altered cell wall permeability.IMPORTANCEThe stringent response, involving the regulatory molecules inorganic polyphosphate [poly(P)] and (p)ppGpp, is believed to mediateMycobacterium tuberculosispersistence. In this study, we identified a novel enzyme (Rv1026, PPX2) responsible for hydrolyzing long-chain poly(P). A genetically engineered M. tuberculosis strain deficient in theppx2gene showed increased poly(P) levels, which were associated with early bacterial growth arrest and reduced susceptibility to the first-line drug isoniazid, as well as increased bacterial survival during exposure to stress conditions and within macrophages. Relative to the control strain, the mutant showed increased thickness of the cell wall and reduced drug permeability. Global gene expression and metabolite analysis revealed reduced expression of the transcriptional and translational machinery and a shift in carbon source utilization. In summary, regulation of the poly(P) balance is critical for persister formation in M. tuberculosis.

Cell Wall Permeability of Pinto Bean Cotyledon Cells Regulates In Vitro Fecal Fermentation and Gut Microbiota

2021 ◽  
Author(s):  
Yanrong Huang ◽  
Sushil Dhital ◽  
Feitong Liu ◽  
Xiong Fu ◽  
Qiang Huang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Changes ◽  
Microbiota Composition ◽  
Colonic Fermentation ◽  
Pinto Bean ◽  
Wall Permeability ◽  
Cell Wall Permeability ◽  
Cotyledon Cells ◽  
Whole Foods

Processing induced structural changes of whole foods on regulation of colonic fermentation rate and microbiota composition are least understood and often overlooked. In the present study, intact cotyledon cells from...

scholarly journals Carbon Source-Induced Modifications in the Mycolic Acid Content and Cell Wall Permeability of Rhodococcus erythropolis E1

2003 ◽  
Vol 69 (12) ◽  
pp. 7019-7027 ◽  
Author(s):  
Ivana Sokolovská ◽  
Raoul Rozenberg ◽  
Christophe Riez ◽  
Paul G. Rouxhet ◽  
Spiros N. Agathos ◽  
...  
Keyword(s):  
Cell Wall ◽  
Carbon Source ◽  
Acid Content ◽  
Rhodococcus Erythropolis ◽  
Mycolic Acid ◽  
Mycolic Acids ◽  
Carbon Chains ◽  
Linear Alkanes ◽  
Wall Permeability ◽  
Cell Wall Permeability

ABSTRACT The influence of the carbon source on cell wall properties was analyzed in an efficient alkane-degrading strain of Rhodococcus erythropolis (strain E1), with particular focus on the mycolic acid content. A clear correlation was observed between the carbon source and the mycolic acid profiles as estimated by high-performance liquid chromatography and mass spectrometry. Two types of mycolic acid patterns were observed after growth either on saturated linear alkanes or on short-chain alkanoates. One type of pattern was characterized by the lack of odd-numbered carbon chains and resulted from growth on linear alkanes with even numbers of carbon atoms. The second type of pattern was characterized by mycolic acids with both even- and odd-numbered carbon chains and resulted from growth on compounds with odd-numbered carbon chains, on branched alkanes, or on mixtures of different compounds. Cellular short-chain fatty acids were twice as abundant during growth on a branched alkane (pristane) as during growth on acetate, while equal amounts of mycolic acids were found under both conditions. More hydrocarbon-like compounds and less polysaccharide were exposed at the cell wall surface during growth on alkanes. Whatever the substrate, the cells had the same affinity for aqueous-nonaqueous solvent interfaces. By contrast, bacteria displayed completely opposite susceptibilities to hydrophilic and hydrophobic antibiotics and were found to be strongly stained by hydrophobic dyes after growth on pristane but not after growth on acetate. Taken together, these data show that the cell wall composition of R. erythropolis E1 is influenced by the nutritional regimen and that the most marked effect is a radical change in cell wall permeability.

scholarly journals A Tetrameric Porin Limits the Cell Wall Permeability ofMycobacterium smegmatis

2002 ◽  
Vol 277 (40) ◽  
pp. 37567-37572 ◽  
Author(s):  
Harald Engelhardt ◽  
Christian Heinz ◽  
Michael Niederweis
Keyword(s):  
Cell Wall ◽  
Wall Permeability ◽  
Cell Wall Permeability

scholarly journals The Activity of Cell-Wall Modifying β-1,3-Glucanases in Soybean Grown in Presence of Heavy Metals

2016 ◽  
Vol 15 (2) ◽  
pp. 114-121
Author(s):  
Monika Bardáčová ◽  
Marína Maglovski ◽  
Zuzana Gregorová ◽  
Yevheniia Konotop ◽  
Miroslav Horník ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Cell Wall ◽  
Cell Walls ◽  
Regulation Mechanism ◽  
Soybean Variety ◽  
Toxic Heavy Metals ◽  
Metal Sequestration ◽  
Enzyme Isoforms ◽  
Wall Permeability ◽  
Cell Wall Permeability

AbstractCell walls represent the first barrier that can prevent the entrance of toxic heavy metals into plants. The composition and the flexibility of the cell wall are regulated by different enzymes. The ß-1,3-glucanases control the degradation of the polysaccharide callose as a flexible regulation mechanism of cell wall permeability and/or its ability to bind metals under stress conditions. The profile and activity of ß-1,3-glucanases in the presence of heavy metals, however, has rarely been studied. Here we studied these enzymes in four soybean varieties (Glycine max) grown in the presence of cadmium ions. These analyses revealed three acidic and one basic enzyme isoforms in each soybean variety, but only two of the acidic isoforms in the variety Moravians were substantially responsive to the presence of Cd2+. Since the responses of certain glucanases were detected mainly in the varieties sensitive to metal and accumulating high amounts of metals, we assume their role in the defense rather than strategic metal sequestration.

Sign in / Sign up

Export Citation Format

Share Document