scholarly journals Adeno-associated Virus Virus-like Particle Characterization via Orthogonal Methods: Nanoelectrospray Differential Mobility Analysis, Asymmetric Flow Field-Flow Fractionation, and Atomic Force Microscopy

ACS Omega ◽  
2021 ◽  
Author(s):  
Samuele Zoratto ◽  
Victor U. Weiss ◽  
Gernot Friedbacher ◽  
Carsten Buengener ◽  
Robert Pletzenauer ◽  
...  
Keyword(s):  
Field Flow Fractionation ◽  
Particle Characterization ◽  
Asymmetric Flow ◽  
Differential Mobility ◽  
Adeno Associated Virus ◽  
Force Microscopy ◽  
Differential Mobility Analysis ◽  
Atomic Force ◽  
Virus Like Particle ◽  
Orthogonal Methods

Aggregation of Fullerol C60(OH)24Nanoparticles as Revealed Using Flow Field-Flow Fractionation and Atomic Force Microscopy

Langmuir ◽  
2010 ◽  
Vol 26 (20) ◽  
pp. 16063-16070 ◽  
Author(s):  
Shoeleh Assemi ◽  
Soheyl Tadjiki ◽  
Bogdan C. Donose ◽  
Anh V. Nguyen ◽  
Jan D. Miller
Keyword(s):  
Atomic Force Microscopy ◽  
Flow Field ◽  
Field Flow Fractionation ◽  
Force Microscopy ◽  
Atomic Force ◽  
Flow Fractionation

Comparative Observation of the Recombinant Adeno-Associated Virus 2 Using Transmission Electron Microscopy and Atomic Force Microscopy

2007 ◽  
Vol 13 (5) ◽  
pp. 384-389 ◽  
Author(s):  
Heng Chen
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Vector System ◽  
Particle Analysis ◽  
Adeno Associated Virus ◽  
Force Microscopy ◽  
Virus Particles ◽  
Atomic Force ◽  
Transmission Electron

Adeno-associated virus (AAV) is a defective, nonpathogenic human parvovirus, which coinfects with a helper adenovirus or herpes virus. AAV's unique characteristics have made it an appealing vector system for gene delivery. AAV or recombinant AAV (rAAV) has been widely detected using negative stain transmission electron microscopy (TEM) but little has been detected using atomic force microscopy (AFM). In this article, we used AFM and TEM to observe the recombinant AAV-2 (rAAV-2) virus particles and applied statistical analysis to the AFM and TEM images. The results indicated that the rAAV-2 particle was a slightly elliptic particle close to round when it was detected by TEM (the mean length of major and minor axes of rAAV-2 particles was 24.77 ± 1.78 nm and 21.84 ± 1.57 nm, respectively), whereas when detected by AFM, the rAAV-2 particle was almost round. Even though the dimensions of the rAAV-2 particle exhibited a polymorphous distribution via off-line particle analysis of AFM, most of the rAAV-2 particles had a mean diameter of approximate 22.04 nm, which was similar to the results obtained by TEM. The results above suggested that AFM was important for accurately determining the average dimensions and distributions of virus particles.

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Sign in / Sign up

Export Citation Format

Share Document