scholarly journals Telmisartan Mitigates TNF-α-Induced Type II Collagen Reduction by Upregulating SOX-9

ACS Omega ◽  
2021 ◽  
Vol 6 (17) ◽  
pp. 11756-11761
Author(s):  
Xiuying Zhang ◽  
Yanfeng Dong ◽  
Hanyu Dong ◽  
Yanhui Cui ◽  
Qing Du ◽  
...  
Keyword(s):  
Type Ii Collagen ◽  
Type Ii ◽  
Tnf Α

scholarly journals Effect of Marine Phytoplankton (Oceanix) and Undenatured Type II Collagen in Combination with Exercise on Endurance Capacity and Oxidative Stress in Rats

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1764-1764
Author(s):  
Kazim Sahin ◽  
Cemal Orhan ◽  
Mehmet Tuzcu ◽  
Nurhan Sahin ◽  
Vijaya Juturu
Keyword(s):  
Antioxidant Enzymes ◽  
Proinflammatory Cytokines ◽  
Type Ii Collagen ◽  
Marine Phytoplankton ◽  
Albino Rats ◽  
High Dose ◽  
Endurance Capacity ◽  
Type Ii ◽  
Tnf Α ◽  
Undenatured Type Ii Collagen

Abstract Objectives To study the effect of exercise training alone and or in combination with marine phytoplankton (Oceanix, OCX) and undenatured type II collagen (UCII) supplementation on the endurance capacity, pro-inflammatory markers, and antioxidant defense markers in rats. Methods A total of 28 male Wistar albino rats were randomly divided into four groups (n  =  7) (i) No exercise and no OCX (Control), (ii) Exercise, (iii) Exercise +OCX-I (2.55 mg d/rat) + UC-II (4 mg), iv) Exercise + OCX-2 (5.1 mg d/rat)+UC-II (4 mg). Levels of cholesterol, triglyceride, proinflammatory cytokines (IL-1β, IL-6, TNF-α, COMP, CRP), lactate and malondialdehyde (MDA) levels activities of antioxidant enzymes were determined in all the groups. Results Run to exhaustion (minutes) improved in the OCX + UC-II treated groups. Levels of cholesterol, triglyceride, proinflammatory cytokines (IL-1β, IL-6, TNF-α, COMP, CRP) decreased by OCX + UC-II supplementation. A significant decrease in lactate and malondialdehyde (MDA) levels and an increase in activities of antioxidant enzymes were observed in the combination of exercise and OCX + UC-II groups. Exercise + OCX + UC-II treated had lower TNF-α and IL-1β levels in muscle than exercise and control rats (P < 0.001). Muscle sterol regulatory element-binding protein 1c (SREBP-1c), liver X receptors (LXR), ATP citrate lyase (ACLY) and fatty acid synthase (FAS) levels in the exercise + OCX + UC-II group were lower than all groups (P < 0.05). The effectiveness of the high dose of OCX was more pronounced than the low dose of OCX. Conclusions These results suggest OCX and UC-II with exercise may enhance lipid metabolism by regulation of gene products involved in lipid and antioxidant metabolism including SREBP-1c, -γ, LXR, ACLY and FAS in rats. Funding Sources Lonza.

scholarly journals Concerted Actions by PIICP, CTXII, and TNF-α in Patients with Juvenile Idiopathic Arthritis

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 648
Author(s):  
Katarzyna Winsz-Szczotka ◽  
Kornelia Kuźnik-Trocha ◽  
Iwona Lachór-Motyka ◽  
Wojciech Lemski ◽  
Krystyna Olczyk
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Joint Destruction ◽  
Treatment Strategies ◽  
Type Ii Collagen ◽  
Diagnostic Tools ◽  
Type Ii ◽  
Process Indicators ◽  
Tnf Α ◽  
Turnover Markers ◽  
Factor Α

Joint destruction in juvenile idiopathic arthritis (JIA), initiated in the early, preclinical stage of the disease, is diagnosed on the basis of clinical evaluation and radiographic imaging. The determination of circulating cartilage-matrix turnover markers can facilitate the diagnosis and application of better and earlier treatment strategies for JIA. We have shown that 96 JIA patients have elevated levels of procollagen II C-terminal propeptide (PIICP), reflecting the extent of joint cartilage biosynthesis, and C-telopeptide of type II collagen (CTXII), a biomarker of the resorption of this tissue. Patients who did not respond to treatment had particularly high levels of these markers. JIA treatment resulted in the normalization of these markers in remissive patients, but not in those with active JIA. We showed correlations between examined variables and inflammatory process indicators, i.e., C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and tumor necrosis factor-α (TNF-α). The TNF-α of patients responding to treatment correlated with PIICP, especially in the patients before treatment (r = 0.898, p < 0.001). Significant changes in serum PIICP during JIA therapy suggest its potential diagnostic utility in the monitoring of disease activity and the possibility of its use in assessing treatment towards remission. Understanding changes in type II collagen metabolism over the course of the discussed arthritis may allow the implementation of both new diagnostic tools and new therapeutic strategies in children with JIA.

Inhibition of GPR17 with pranlukast protects against TNF-α-induced loss of type II collagen in ATDC5 cells

2020 ◽  
Vol 88 ◽  
pp. 106870
Author(s):  
Zhangfu Wang ◽  
Weiwei Zhou ◽  
Guangbin Zheng ◽  
Guangyong Yang
Keyword(s):  
Type Ii Collagen ◽  
Type Ii ◽  
Tnf Α

scholarly journals Niacinamide and undenatured type II collagen modulates the inflammatory response in rats with monoiodoacetate-induced osteoarthritis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kazim Sahin ◽  
Osman Kucuk ◽  
Cemal Orhan ◽  
Mehmet Tuzcu ◽  
Ali Said Durmus ◽  
...  
Keyword(s):  
Joint Pain ◽  
Type Ii Collagen ◽  
Pain Behavior ◽  
Type Ii ◽  
Oral Supplementation ◽  
Protein Levels ◽  
Tnf Α ◽  
Monosodium Iodoacetate ◽  
Undenatured Type Ii Collagen ◽  
Behavior Of Rats

AbstractThe current work aimed to examine the properties of oral supplementation of niacinamide and undenatured type II collagen (UCII) on the inflammation and joint pain behavior of rats with osteoarthritis (OA). Forty-nine Wistar rats were allocated into seven groups; control (no MIA), MIA as a non-supplemental group with monosodium iodoacetate (MIA)-induced knee osteoarthritis, MIA + undenatured type II collagen (UCII) at 4 mg/kg BW, MIA + Niacinamide at 40 mg/kg BW (NA40), MIA + Niacinamide at 200 mg/kg BW (NA200), MIA + UCII + NA40 and MIA + UCII + NA200. Serum IL‐1β, IL‐6, TNF-α, COMP, and CRP increased in rats with OA and decreased in UCII and NA groups (p < 0.05). Rats with osteoarthritis had greater serum MDA and knee joint MMP-3, NF-κB, and TGβ protein levels and decreased in treated groups with UCII and NA (p < 0.05). The rats with OA also bore elevated joint diameters with joint pain behavior measured as decreased the stride lengths, the paw areas, and the paw widths, and increased the Kellgren-Lawrence and the Mankin scores (p < 0.05) and decreased in UCII treated groups. These results suggest the combinations with the UCII + NA supplementation as being most effective and reduce the inflammation responses for most OA symptoms in rats.

Anti-inflammatory Effect of Bee Venom on Type II Collagen-Induced Arthritis

2004 ◽  
Vol 32 (03) ◽  
pp. 361-367 ◽  
Author(s):  
Jae-Dong Lee ◽  
Su-Young Kim ◽  
Tae-Woo Kim ◽  
Sang-Hoon Lee ◽  
Hyung-In Yang ◽  
...  
Keyword(s):  
Acupuncture Therapy ◽  
Acupuncture Point ◽  
Type Ii Collagen ◽  
Bee Venom ◽  
Control Group ◽  
Type Ii ◽  
Collagen Induced Arthritis ◽  
Collagen Injection ◽  
Tnf Α ◽  
Anti Inflammatory

Bee venom (BV) has been used to relieve pain and reduce inflammation in traditional Oriental medicine, especially in chronic inflammatory diseases such as rheumatoid arthritis (RA). We previously reported that the BV injection into a traditional acupuncture point (Zusanli) reduced arthritis-associated edema and nociceptive responses in Freund's adjuvant-induced arthritis in rats (Kwon et al., 2001). This study was designed to evaluate the anti-inflammatory and anti-cytokine effect of BV on a murine type-II collagen-induced arthritis (CIA) model. Male mice were immunized by spontaneous injection of 100 μg of an emulsion of bovine type-II collagen and complete Freund's adjuvant (CFA), with a booster injection after 2 weeks. In the experimental group, 0.1 ml BV was injected at acupuncture point (Zusanli) near both knees twice a week for a total of 5 times. In the control group, normal saline was injected at the same frequencies. These injections began 5 weeks after the first collagen injection. Starting the 3rd week after the first collagen injection, we examined limb swelling and severity of arthritis twice a week. At 8 weeks, mice were sacrificed and synovial tissue was examined with the light microscope and serum cytokines (IL-1β and TNF-α) were measured by ELISA. The incidence of arthritis, the mean arthritis index and the number of arthritic limbs were significantly lower in the treatment compared to the control group (63% versus 75%, 3.4% versus 8.5%, 23% versus 75%, respectively). Among the serum proinflammatory cytokines, the production of TNF-α in the BV group was suppressed compared to the control group (59+/-4.5 versus 99.5+/-6.5, p <0.05), but IL-1β was not suppressed. The examination of the histopathology of the joints of murine CIA showed decreased inflammation signs and less lymphocyte infiltration after BV acupuncture therapy. Acupuncture therapy with BV suppressed the development of arthritis and caused inhibition of the immune responses in type-II collagen-induced arthritis.

scholarly journals Effects of Exercise Combined with Undenatured Type II Collagen on Endurance Capacity, Antioxidant Status, Muscle Lipogenic Genes and E3 Ubiquitin Ligases in Rats

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 851
Author(s):  
Cemal Orhan ◽  
Emre Sahin ◽  
Besir Er ◽  
Mehmet Tuzcu ◽  
Andrey P. Lopes ◽  
...  
Keyword(s):  
Antioxidant Status ◽  
Type Ii Collagen ◽  
Serum Lactate ◽  
Creatinine Kinase ◽  
Control Group ◽  
Endurance Capacity ◽  
Type Ii ◽  
Sedentary Control ◽  
Tnf Α ◽  
Undenatured Type Ii Collagen

The current study aimed to investigate the effect of exercise combined with undenatured type II collagen (UCII) administration on endurance capacity, lipid metabolism, inflammation, and antioxidant status in rats. Twenty-one male Wistar albino rats were divided into three groups as follows: (1) Sedentary control, (2) Exercise (E), (3) Exercise + UCII (4 mg/kg BW/day; E + UCII). The findings showed that the exhaustive running time in the UCII group was significantly prolonged compared to that of the non-supplemented group (p < 0.001). When compared to the control group, total serum cholesterol (TC, p < 0.05) and triglyceride (TG, p < 0.05) levels decreased, while creatinine kinase (CK) levels increased in the E group (p < 0.001). Serum creatinine kinase levels were reduced in the E + UCII group compared to the E group (p < 0.01). Serum lactate, myoglobin (p < 0.01), and osteocalcin levels (p < 0.01) increased significantly in exercised rats compared to sedentary control rats, while serum lactate (p < 0.01) and myoglobin (p < 0.0001) levels decreased in the E + UCII group compared to control. Additionally, UCII supplementation caused significant increases in antioxidant enzyme activities [SOD (p < 0.01) and GSH-Px (p < 0.05)] and decreases in malondialdehyde (MDA) and tumor necrosis factor (TNF-α) levels (p < 0.001). Muscle lipogenic protein (SREBP-1c, ACLY, LXR, and FAS) levels were lower in the E + UCII group than in other groups. In addition, UCII supplementation decreased muscle MAFbx, MuRF-1, myostatin and increased MyoD levels in exercised rats. Moreover, the E + UCII group had lower muscle inflammatory markers [TNF-α (p < 0.0001) and IL-1β (p < 0.01)] than the control group. These results suggest exercise combined with UCII (4 mg/kg BW/day) modulates lipid, muscle, and antioxidant status in rats.

scholarly journals Anti-arthritic effect of total anthraquinone from Polygonum cuspidatum on type II collagen-induced arthritis in rats

2017 ◽  
Vol 16 (10) ◽  
pp. 2453-2459
Author(s):  
Feng Wang ◽  
Yan-hong Qiao ◽  
Hui-min Niu ◽  
Hong Zhao
Keyword(s):  
Inflammatory Cytokines ◽  
Type Ii Collagen ◽  
Serum Levels ◽  
Underlying Mechanism ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type Ii ◽  
Polygonum Cuspidatum ◽  
Collagen Induced Arthritis ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

Purpose: To study the anti-arthritic effect of total anthraquinone from Polygonum  cuspidatum (TAPC) on type II collagen-induced arthritis (CIA) in rats, and to  investigate the underlying mechanism(s).Methods: CIA rats were prepared and treated orally with TAPC at doses of 50, 100 and 200 mg/kg/day, for 24 days. Paw volume and arthritis score were measured prior to TAPC treatment, and subsequently at 3-day intervals on days 3, 6, 9, 12, 15, 18, 21 and 24. Serum levels of TNF-α, IL-6 and IL-17 were determined by enzyme-linked immunosorbent assay (ELISA), while synovial tissue TNF-α, IL-6 and IL-17mRNA expressions were assayed by real time-polymerase chain reaction (RT-PCR). Thymus and spleen indices were also determined.Results: TAPC (50, 100 and 200 mg/kg) significantly alleviated paw swelling (p < 0.05), arthritis scores (p < 0.05) and thymus and spleen indices (p < 0.05) of CIA rats, when compared with the control rats. In addition, TAPC significantly decreased serum levels of the pro-inflammatory cytokines TNF-α, IL-6 and IL-17 (p < 0.01); and down-regulated their mRNA expressions in synovial tissues (p < 0.01).Conclusion: These results suggest that TAPC exerts good anti-arthritic activity in rats, most probably via suppression of inflammatory responses.Keywords: Polygonum cuspidatum, Anthraquinone, Type II collagen-induced  arthritis, Pro-inflammatory cytokines

scholarly journals Changes in Synovial Fluid Inflammatory Mediators and Cartilage Biomarkers After Experimental Acute Equine Synovitis

2015 ◽  
Vol 59 (1) ◽  
pp. 129-134
Author(s):  
Guanying Wang ◽  
Xinran Li ◽  
Renli Jiang ◽  
Yue Li ◽  
Xiaojing Fan ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Baseline Level ◽  
Time Course ◽  
White Blood Cells ◽  
Type Ii Collagen ◽  
Inflammatory Biomarkers ◽  
Type Ii ◽  
Tnf Α ◽  
And Cartilage ◽  
Cartilage Biomarkers

Abstract The purpose of the study was to define transient changes in the concentration of inflammatory biomarkers and cartilage biomarkers in the synovial fluid of joints following experimentally induced acute equine synovitis. Acute synovitis was induced in eight skeletally mature mares by a sterile intra-articular injection of 1 mL of phosphate-buffered saline (PBS) containing 0.5 ng of lipopolysaccharide (LPS). The solution was injected into the right middle carpal joint. One mL of sterile PBS was injected into the left control joint. Synovial fluid was obtained at the baseline level and at 8, 24, and 168 h after injection. The levels of inflammatory biomarkers-prostaglandin E2 (PGE2), interleukin 1β (IL-1β), and tumour necrosis factor-α (TNF-α), and cartilage turnover biomarkers-collagenase-cleavage neoepitope of type II collagen (C2C) and C-terminal crosslinked telopeptide type II collagen (CTX-II) were detected with proper assays. Single injections of LPS raised the number of synovial white blood cells and concentrations of total protein, PGE2, IL-1β, TNF-α, C2C, and CTX-II. PGE2 and IL-1β rose sharply at 8 h, while TNF-α increased steadily through 8 h and 24 h, at that point; these three factors returned to the baseline level by 168 h. The time course of C2C and CTX-II concentrations peaked sharply at 24 h, and continued to be significantly elevated over the baseline level even at 168 h. Injections of LPS into the joints led to a temporal inflammatory response, which in turn increased local release of inflammatory biomarkers and significantly altered the concentrations of cartilage markers in the synovial fluid.

scholarly journals Effect of infusion irrigation with different irrigating solutions on transient receptor potential vanilloid 5 and intra-articular inflammation in a post-traumatic osteoarthritis rabbit model

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Xinghui Liu ◽  
Rong Chen ◽  
Liangbo Jiang ◽  
Xiangwei Li ◽  
Zhibo Sun
Keyword(s):  
Magnesium Sulfate ◽  
Type Ii Collagen ◽  
Cartilage Degeneration ◽  
The Other ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Type Ii ◽  
Tnf Α ◽  
Trap 5B ◽  
Post Traumatic

Abstract Background The incidence of post-traumatic osteoarthritis (PTOA) after anterior cruciate ligament reconstruction (ACLR) is high, but there is still a lack of intra-operative preventive measures. This study aimed to evaluate the effect of different irrigating solutions continuous irrigation on intra-articular inflammation and cartilage degeneration. Methods 66 New Zealand rabbits were randomly divided into normal (N) group, no treatment (NT) group, sodium chloride (NaCl) group, magnesium sulfate (MgSO4) group, and calcium chloride (CaCl2) group. The right knee joint of the experimental group was utilized to construct the model of PTOA, and the left side was utilized as the normal control group. At different time points postoperatively, the blood concentration of hemoglobin and Mg2 + , the synovial fluid concentration of IL-1 β, TNF-α, tartrate-resistant acid phosphatase-5b (TRAP-5b), and Type II Collagen, the gene expression of IL-1 β and MMP-3, and the protein expression of TRPV5 and CaM were detected. Pearson′s linear correlation was employed to identify the possible relationship between the expression of TRAP-5b and the expression of IL-1β, IL-6, TNF-α, and Type II collagen. The hematoxylin and eosin staining (HE), Masson’s trichrome staining, and Alcian blue staining were performed at postoperative 35 days. Osteoarthritis Scoring (OA score) comprised categories including Alcian blue staining, cartilage histology, the cellular density of cartilage, degree of cell disintegration, and formation of chondrocyte cluster were blindly scored by trained researchers at postoperative 35 days. Results There was no statistical difference (P > 0.05) in the hemoglobin concentration between different groups. The concentration of serum Mg2+ in the MgSO4 group was higher than that of the other three groups (P < 0.05) on the same day of operation, then gradually decreased. The expression of IL-1 β, IL-6, and TRAP-5b in synovial fluid increased 5 days after the operation, decreased at 15 days, and then increased again with time in the NT group, NaCl group, and NT group and NaCl group. At 35 days after the operation, the expression of IL-1 β, IL-6, TRAP-5b, and type II collagen in the MgSO4 group were lower than that in the other three groups (except group N) (P < 0.05).The correlation analysis results showed that the TRAP-5b levels correlated positively with IL-1 β, IL-6, TNF-α, and type II collagen concentrations. The histological examination revealed that the surface smoothness of cartilage, the morphology of chondrocytes, the arrangement of collagen fibers, and the density of proteoglycan in the MgSO4 group were better than those in other experimental groups. At 35 days postoperatively, the gene expression of IL-1 β and MMP-3 and the protein expression of CaM and TRPV5 in synovium in the MgSO4 group was lower than that in the NaCl group and CaCl2 group. Conclusion Intra-operative irrigation with magnesium sulfate solution can inhibit the inflammatory factors and the expression of TRPV5, which can also reduce collagen loss and delay cartilage degeneration. Therefore, the use of magnesium sulfate in intra-operative irrigation may be an ideal choice to prevent PTOA.

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