scholarly journals Proteomics Study on the Cerebrospinal Fluid of Patients with Encephalitis

ACS Omega ◽  
2021 ◽  
Author(s):  
Liu-Lin Xiong ◽  
Lu-Lu Xue ◽  
Yan-Jun Chen ◽  
Ruo-Lan Du ◽  
Qian Wang ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Proteomics Study

Proteomics Study of Mesenchymal Stem Cell-Like Cells Isolated from Cerebrospinal Fluid of Patients with Meningioma

2019 ◽  
Vol 16 (4) ◽  
pp. 282-288
Author(s):  
Arash Saffarian ◽  
Amir Tarokh ◽  
Mohammad Reza Haghshenas ◽  
Mousa Taghipour ◽  
Nooshafarin Chenari ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Cancer Progression ◽  
Flow Cytometer ◽  
Differentially Expressed ◽  
Phosphoglycerate Mutase ◽  
Intracranial Meningioma ◽  
Type I ◽  
Differentially Expressed Proteins ◽  
Isolated Cells ◽  
Proteomics Study

Background:Cerebrospinal fluid (CSF) contains pro-growth factors that can affect proliferation, migration and differentiation of Mesenchymal Stem Cells (MSCs).Objective:This study aimed to isolate MSC like cells from CSF of patients with meningioma and psudotumorcerebri (PTC) and identify differentially expressed proteins in these cells.Methods:Five patients with newly diagnosed intracranial meningioma and five patients with PTC were recruited in this comparative proteomics study. MSCs were isolated from CSF and validated by mesenchyml and non-mesenchyml fluorochrome antibodies, and flow cytometer analysis. Two- Dimensional Gel Electrophoresis (2-DE) coupled with Mass Spectrometry (MS) was performed to identify differentially expressed proteins.Results:Microscopic views of the isolated cells as well as flow cytometer analysis were found to be compatible with MSC-like cells. Eight distinct protein spots were differentially and reproducibly expressed among the stained gels of two studied groups. The identified proteins were Phosphoglycerate Mutase 1 (PGAM1), LIM and SH3 domain protein (LASP1), peroxiredoxin-6 (PRDX-6), type I cytoskeletal 9 (KRT9), Superoxide Dismutase (SOD), endoplasmin, Stathmin 1 (STMN1), and glutathione S-transferase (GST).Conclusion:This study provides new insights into the plausible role of CSF derived MSCs in cancer progression, and reveals a promising therapeutic opportunity for targeting of MSC proteins in patients with meningioma.

In search of cerebrospinal fluid biomarkers of fatigue in multiple sclerosis: A proteomics study

2018 ◽  
Vol 28 (3) ◽  
pp. e12721 ◽  
Author(s):  
Philipp O. Valko ◽  
Bernd Roschitzki ◽  
Wolfgang Faigle ◽  
Jonas Grossmann ◽  
Christian Panse ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Cerebrospinal Fluid Biomarkers ◽  
Proteomics Study
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