scholarly journals PLLA Porous Microsphere-Reinforced Silk-Based Scaffolds for Auricular Cartilage Regeneration

ACS Omega ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 3372-3383
Author(s):  
Yan Zeng ◽  
Xiaokai Li ◽  
Xia Liu ◽  
Yuzhou Yang ◽  
Zhimin Zhou ◽  
...  
Keyword(s):  
Cartilage Regeneration ◽  
Auricular Cartilage ◽  
Porous Microsphere

scholarly journals Comparative Analysis of Adipose-Derived Stromal Cells and Their Secretome for Auricular Cartilage Regeneration

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Se-Joon Oh ◽  
Kyung-Un Choi ◽  
Sung-Won Choi ◽  
Sung-Dong Kim ◽  
Soo-Keun Kong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Stromal Cells ◽  
Collagen Type ◽  
Cartilage Regeneration ◽  
Cartilage Defects ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Auricular Cartilage ◽  
Adipose Derived Stromal Cells

Adipose-derived stromal cells (ADSCs) can repair auricular cartilage defects. Furthermore, stem cell secretome may also be a promising biological therapeutic option, which is equal to or even superior to the stem cell. We explored the therapeutic efficacies of ADSCs and their secretome in terms of rabbit auricular cartilage regeneration. ADSCs and their secretome were placed into surgically created auricular cartilage defects. After 4 and 8 weeks, the resected auricles were histopathologically and immunohistochemically examined. We used real-time PCR to determine the levels of genes expressing collagen type II, transforming growth factor-β1 (TGF-β1), and insulin-like growth factor-1 (IGF-1). ADSCs significantly improved auricular cartilage regeneration at 4 and 8 weeks, compared to the secretome and PBS groups, as revealed by gross examination, histopathologically and immunohistochemically. ADSCs upregulated the expression of collagen type II, TGF-β1, and IGF-1 more so than did the secretome or PBS. The expression levels of collagen type II and IGF-1 were significantly higher at 8 weeks than at 4 weeks after ADSC injection. Although ADSCs thus significantly enhanced new cartilage formation, their secretome did not. Therefore, ADSCs may be more effective than their secretome in the repair of auricular cartilage defect.

scholarly journals Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in Rabbits

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Se-Joon Oh ◽  
Hee-Young Park ◽  
Kyung-Un Choi ◽  
Sung-Won Choi ◽  
Sung-Dong Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cartilage Defect ◽  
Collagen Type ◽  
Cartilage Regeneration ◽  
Control Group ◽  
Adipose Derived Stem Cells ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Auricular Cartilage

Tissue engineering cell-based therapy using induced pluripotent stem cells and adipose-derived stem cells (ASCs) may be promising tools for therapeutic applications in tissue engineering because of their abundance, relatively easy harvesting, and high proliferation potential. The purpose of this study was to investigate whether ASCs can promote the auricular cartilage regeneration in the rabbit. In order to assess their differentiation ability, ASCs were injected into the midportion of a surgically created auricular cartilage defect in the rabbit. Control group was injected with normal saline. After 1 month, the resected auricles were examined histopathologically and immunohistochemically. The expression of collagen type II and transforming growth factor-β1 (TGF-β1) were analyzed by quantitative polymerase chain reaction. Histopathology showed islands of new cartilage formation at the site of the surgically induced defect in the ASC group. Furthermore, Masson’s trichrome staining and immunohistochemistry for S-100 showed numerous positive chondroblasts. The expression of collagen type II and TGF-β1 were significantly higher in the ASCs than in the control group. In conclusion, ASCs have regenerative effects on the auricular cartilage defect of the rabbit. These effects would be expected to contribute significantly to the regeneration of damaged cartilage tissue in vivo.

Biocompatibility evaluation of densified bacterial nanocellulose hydrogel as an implant material for auricular cartilage regeneration

2014 ◽  
Vol 98 (17) ◽  
pp. 7423-7435 ◽  
Author(s):  
Héctor Martínez Ávila ◽  
Silke Schwarz ◽  
Eva-Maria Feldmann ◽  
Athanasios Mantas ◽  
Achim von Bomhard ◽  
...  
Keyword(s):  
Cartilage Regeneration ◽  
Auricular Cartilage ◽  
Bacterial Nanocellulose ◽  
Implant Material

scholarly journals Inhibition of apoptosis signal-regulating kinase 1 alters the wound epidermis and enhances auricular cartilage regeneration

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0185803 ◽  
Author(s):  
Qian-Shi Zhang ◽  
Deepa S. Kurpad ◽  
My G. Mahoney ◽  
Marla J. Steinbeck ◽  
Theresa A. Freeman
Keyword(s):  
Cartilage Regeneration ◽  
Auricular Cartilage ◽  
Wound Epidermis

scholarly journals Stereoscopic visualization and quantification of auricular cartilage regeneration in rabbits using multiphoton microscopy

Scanning ◽  
2014 ◽  
Vol 36 (5) ◽  
pp. 540-546 ◽  
Author(s):  
Jing Chen ◽  
Xiaoqin Zhu ◽  
Yahao Xu ◽  
Yiyan Tang ◽  
Shuyuan Xiong ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Cartilage Regeneration ◽  
Auricular Cartilage ◽  
Stereoscopic Visualization

3D bioprinting of human chondrocyte-laden nanocellulose hydrogels for patient-specific auricular cartilage regeneration

Bioprinting ◽  
2016 ◽  
Vol 1-2 ◽  
pp. 22-35 ◽  
Author(s):  
Héctor Martínez Ávila ◽  
Silke Schwarz ◽  
Nicole Rotter ◽  
Paul Gatenholm
Keyword(s):  
Cartilage Regeneration ◽  
Patient Specific ◽  
3D Bioprinting ◽  
Auricular Cartilage ◽  
Human Chondrocyte

scholarly journals The Potential Use of Platelet-Rich Plasma to Reconstruct the Microtia Chondrocyte in Human Auricular Cartilage Regeneration

2015 ◽  
Vol 2015 ◽  
pp. 1-7
Author(s):  
Wei-Hong Chen ◽  
Hen-Yu Liu ◽  
Ching-Yu Tsai ◽  
Chia-che Wu ◽  
Hong-Jian Wei ◽  
...  
Keyword(s):  
Platelet Rich Plasma ◽  
Collagen Scaffold ◽  
Cartilage Regeneration ◽  
Type Ii Collagen ◽  
Surgical Reconstruction ◽  
Auricular Cartilage ◽  
3 Dimensional ◽  
Potential Use ◽  
Specific Mrna

Microtia is characterized as an incomplete auricular development and surgical reconstruction for microtia is still limited even with emerging developments. This study aimed to apply bionanomaterials (PRP/collagen scaffold) for human auricular neocartilage reconstruction by using microtia chondrocytes. The results showed that PRP (TGF-β1 750 pg/mL and 1 ng/mL) increased cell viability of microtia chondrocytes during in vitro 9-day cultures. Additionally, chondrogenic-specific mRNA of Aggrecan and type II collagen (Col II) was significantly and continuously expressed with PRP treatment during the 21-day in vitro expansion. Tissue engineering of auricular neocartilage was performed by seeding microtia chondrocytes in bionanomaterials (PRP/collagen scaffold) 3-dimensional (3D) cultures. Immunohistochemistry (IHC) of Col II showed intensive signals between cells and matrix after 4-week cultures. Conclusion. Our results demonstrated that PRP promotes proliferation and redifferentiation of microtia chondrocytes and provides regenerative potentials in auricular neocartilage reconstruction.

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