scholarly journals Optoacoustic/Fluorescent/Acoustic Imaging Probe Based on Air-Filled Bubbles Functionalized with Gold Nanorods and Fluorescein Isothiocyanate

ACS Omega ◽  
2021 ◽  
Vol 6 (5) ◽  
pp. 3809-3821
Author(s):  
Roman A. Barmin ◽  
Polina G. Rudakovskaya ◽  
Vasiliy S. Chernyshev ◽  
Olga I. Guslyakova ◽  
Pavel A. Belcov ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Fluorescein Isothiocyanate ◽  
Acoustic Imaging ◽  
Imaging Probe

scholarly journals Visualization of Protease Activity In Vivo Using an Activatable Photo-Acoustic Imaging Probe Based on CuS Nanoparticles

Theranostics ◽  
2014 ◽  
Vol 4 (2) ◽  
pp. 134-141 ◽  
Author(s):  
Kai Yang ◽  
Lei Zhu ◽  
Liming Nie ◽  
Xiaolian Sun ◽  
Liang Cheng ◽  
...  
Keyword(s):  
Protease Activity ◽  
Acoustic Imaging ◽  
Imaging Probe

Physiological Effects of Nonimmune Platelet Associated Immunoglobulin G

1981 ◽  
Vol 45 (01) ◽  
pp. 027-033 ◽  
Author(s):  
K Sugiura ◽  
M Steiner ◽  
M Baldini
Keyword(s):  
Shape Change ◽  
Fluorescein Isothiocyanate ◽  
Recovery Phase ◽  
Human Platelets ◽  
Serotonin Release ◽  
Platelet Volume ◽  
Igg Antibody ◽  
Heterologous Antiserum ◽  
Igg Binding ◽  
Series Of Experiments

SummaryThe function of nonimmune IgG associated with platelets is unknown. In a series of experiments we have investigated this problem, relating amount of platelet-associated IgG (PAIgG) to platelet volume, serotonin release, adherence of platelets to monocytes and platelet senescence. Most of these studies were performed with human platelets. Platelets freed of preexisting PAIgG by incubation at 22° C were incubated with IgG in a series of concentrations ranging from 0.4 — 27.0 X10-6 M. The IgG preparations used were demonstrably free of aggregated forms of the protein. The amount of PAIgG bound to platelets was determined by the use of fluorescein isothiocyanate-conjugated anti-IgG antibody (F-anti-IgG antibody) which was quantified in a fluorospectrophotometer. Newly bound IgG was assayed similarly by the use of F-IgG. A dose-dependent increase in platelet volume was associated with the binding of nonimmune IgG by platelets. The process which leveled off at an IgG concentration of 1.2 —1.5 X10-5 M was almost fully reversible and was not due to platelet shape change or aggregation. Release of serotonin from IgG-treated platelets was relatively small but to the extent that it occurred was positively related to the IgG concentration to which platelets were exposed. Adherence to autologous monocytes studied quantitatively by the use of formaldehyde-fixed cells was also positively related to the amount of IgG on the platelets. Normal or IgG-defident serum had a potent inhibitory (noncompetitive) action on the binding of F-IgG and F-anti-human IgG antibody to human platelets. Cohorts of platelets prepared in rabbits during the recovery phase of immunological thrombocytopenia induced by injection of heterologous antiserum, showed an age-dependent increase of PAIgG and of IgG binding. These results suggest that PAIgG plays a role in the clearance of senescent platelets.

Interspecific variation of zona pellucida glycoconjugates in several species of marsupial

Reproduction ◽  
2000 ◽  
pp. 111-120 ◽  
Author(s):  
JA Chapman ◽  
OW Wiebkin ◽  
WG Breed
Keyword(s):  
Glycine Max ◽  
Sialic Acid ◽  
Zona Pellucida ◽  
Fluorescein Isothiocyanate ◽  
Interspecific Variation ◽  
Lectin Histochemistry ◽  
Sialic Acids ◽  
Arachis Hypogea ◽  
Marsupial Species ◽  
Similar Intensity

The zona pellucida glycoconjugate content of several marsupial species was investigated using differential lectin histochemistry. Ovaries from fat-tailed dunnarts, a southern brown bandicoot, grey short-tailed opossums, brushtail possums, ringtail possums, koalas and eastern grey kangaroos were fixed, embedded in paraffin wax, sectioned and stained with ten fluorescein isothiocyanate-conjugated lectins. Sections were also incubated with either neuraminidase or saponified, respectively, before incubation with the lectins to identify saccharide residues masked by sialic acids or O-acetyl groups on sialic acids. The zonae pellucidae surrounding the oocytes of the marsupials demonstrated interspecific variation in glycoconjugate content, with mannose-containing glycoconjugates exhibiting the greatest variation. Some of the zona pellucida glycoconjugates of all species, except those of the opossums, were masked by sialic acid with an increase in fluorescence with lectins from Arachis hypogea (PNA), and Glycine max (SBA), after desialylation. The disaccharide beta-galactose(1-4)N-acetyl-D-glucosamine appeared to be conformationally masked by O-acetyl groups of sialic acids in the zonae pellucidae of all species, with an increase in fluorescence with the lectin from Erythrina cristagalli (ECA), after saponification. Similar intensity and localization of beta-(1-4)-N-acetyl-D-glucosamine, as shown by staining of the lectin from Triticum vulgaris (WGA), to the inner and outer regions of the zona pellucida, were found to those reported in eutherian species. WGA fluorescence became uniform throughout the zonae pellucidae after saponification, indicating differential O-acetylation of sialic acids on the internal compartment of the zonae pellucidae.

Development of in situ Imaging Probe for Surgical Operation of Deep Brain Stimulation

2011 ◽  
Vol 131 (12) ◽  
pp. 427-428
Author(s):  
Toshihiko Noda ◽  
Pan Yi-Li ◽  
Ayato Tagawa ◽  
Takuma Kobayashi ◽  
Kiyotaka Sasagawa ◽  
...  
Keyword(s):  
Deep Brain Stimulation ◽  
Brain Stimulation ◽  
Surgical Operation ◽  
Imaging Probe ◽  
Deep Brain ◽  
In Situ Imaging

1969-P: Time-Based Internalized Characterization of Exendin-4-Based Glucagon-Like Peptide-1 Receptor-Targeting Imaging Probe

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 1969-P
Author(s):  
TAKAAKI MURAKAMI ◽  
HIROYUKI FUJIMOTO ◽  
NAOTAKA FUJITA ◽  
KEITA HAMAMATSU ◽  
JUNJI FUJIKURA ◽  
...  
Keyword(s):  
Receptor Targeting ◽  
Imaging Probe ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

Synthesis and Characterization of Polydopamine-Coated Gold Nanorods

2017 ◽  
Vol 62 (1-2) ◽  
pp. 23-31
Author(s):  
L.C. Şuşu ◽  
◽  
A.M. Crăciun ◽  
S. Aştilean ◽  
◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Synthesis And Characterization
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