scholarly journals Computational Prediction of Protein Arginine Methylation Based on Composition–Transition–Distribution Features

ACS Omega ◽  
2020 ◽  
Vol 5 (42) ◽  
pp. 27470-27479
Author(s):  
Ruiyan Hou ◽  
Jin Wu ◽  
Lei Xu ◽  
Quan Zou ◽  
Yi-Jun Wu
Keyword(s):  
Computational Prediction ◽  
Arginine Methylation ◽  
Transition Distribution ◽  
Distribution Features ◽  
Protein Arginine Methylation

scholarly journals Protein arginine methylation during lytic adenovirus infection

2004 ◽  
Vol 383 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Julia KZHYSHKOWSKA ◽  
Elisabeth KREMMER ◽  
Markus HOFMANN ◽  
Hans WOLF ◽  
Thomas DOBNER
Keyword(s):  
Cell Function ◽  
Arginine Methylation ◽  
Productive Infection ◽  
Mrna Metabolism ◽  
Infected Cells ◽  
Protein Arginine Methylation ◽  
Adenovirus E1b ◽  
Protein Arginine Methyltransferase 1

Arginine methylation of proteins affects major processes in the cell, including transcriptional regulation, mRNA metabolism, signal transduction and protein sorting. Arginine methylation of Ad (adenovirus) E1B 55-kDa-associated protein E1B-AP5 was recently described by us [Kzhyshkowska, Schutt, Liss, Kremmer, Stauber, Wolf and Dobner (2001) Biochem. J. 358, 305–314]. In this first example of protein arginine methylation analysis in Ad-infected cells, we investigated methylation of the E1B-AP5 and the viral L4-100 kDa protein. We demonstrate that E1B-AP5 methylation is enhanced during the course of infection in a cell-type-specific manner. We also show that L4-100 kDa is efficiently methylated in Ad-infected cells. L4-100 kDa formed complex with methyltransferase in vivo during productive infection, and can be methylated by HRMT1L2 (human protein arginine methyltransferase 1) in vitro. Comparative analysis of E1B-AP5 and L4-100 kDa protein methylation in Ad-infected HeLa, MCF-7 and H1299 cells revealed that the profile of protein arginine methylation correlates with the efficiency of Ad proteins production. Our results suggest that protein arginine methylation is an important host-cell function required for efficient Ad replication.

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