scholarly journals Mechanisms of Insecticidal Action of Metarhizium anisopliae on Adult Japanese Pine Sawyer Beetles (Monochamus alternatus)

ACS Omega ◽  
2020 ◽  
Vol 5 (39) ◽  
pp. 25312-25318
Author(s):  
Ho Myeong Kim ◽  
Seul-Gi Jeong ◽  
In Seong Choi ◽  
Jung Eun Yang ◽  
Kwang Ho Lee ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Monochamus Alternatus ◽  
Insecticidal Action ◽  
Japanese Pine Sawyer

Colonization of Metarhizium anisopliae on the surface of pine tree logs: A promising biocontrol strategy for the Japanese pine sawyer, Monochamus alternatus

2020 ◽  
Vol 124 (2) ◽  
pp. 125-134
Author(s):  
Jong Cheol Kim ◽  
Sehyeon Baek ◽  
So Eun Park ◽  
Sihyeon Kim ◽  
Mi Rong Lee ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Monochamus Alternatus ◽  
Pine Tree ◽  
Japanese Pine Sawyer

scholarly journals Transcriptome Analysis of the Japanese Pine Sawyer Beetle, Monochamus alternatus, Infected with the Entomopathogenic Fungus Metarhizium anisopliae JEF-197

2021 ◽  
Vol 7 (5) ◽  
pp. 373
Author(s):  
Jong-Cheol Kim ◽  
Mi-Rong Lee ◽  
Sihyeon Kim ◽  
So-Eun Park ◽  
Se-Jin Lee ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Ribosome Biogenesis ◽  
De Novo ◽  
Differentially Expressed Gene ◽  
Rna Seq ◽  
Monochamus Alternatus ◽  
Fungal Treatment ◽  
Chemical Agents ◽  
Japanese Pine Sawyer ◽  
Immune Related Genes

The Japanese pine sawyer (JPS) beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae), damages pine trees and transmits the pine wilt nematode, Bursaphelenchus xylophilus Nickle. Chemical agents have been used to control JPS beetle, but due to various issues, efforts are being made to replace these chemical agents with entomopathogenic fungi. We investigated the expression of immune-related genes in JPS beetle in response to infection with JEF-197, a Metarhizium anisopliae isolate, using RNA-seq. RNA samples were obtained from JEF-197, JPS adults treated with JEF-197, and non-treated JPS adults on the 8th day after fungal treatment, and RNA-seq was performed using Illumina sequencing. JPS beetle transcriptome was assembled de novo and differentially expressed gene (DEG) analysis was performed. There were 719 and 1953 up- and downregulated unigenes upon JEF-197 infection, respectively. Upregulated contigs included genes involved in RNA transport, ribosome biogenesis in eukaryotes, spliceosome-related genes, and genes involved in immune-related signaling pathways such as the Toll and Imd pathways. Forty-two fungal DEGs related to energy and protein metabolism were upregulated, and genes involved in the stress response were also upregulated in the infected JPS beetles. Together, our results indicate that infection of JPS beetles by JEF-197 induces the expression of immune-related genes.

Complete mitochondrial genome of the Japanese pine sawyer,Monochamus alternatus(Coleoptera: Cerambycidae)

2014 ◽  
Vol 27 (2) ◽  
pp. 1144-1145 ◽  
Author(s):  
Fengbo Li ◽  
Huixian Zhang ◽  
Wei Wang ◽  
Hongbiao Weng ◽  
Zhiqi Meng
Keyword(s):  
Mitochondrial Genome ◽  
Complete Mitochondrial Genome ◽  
Monochamus Alternatus ◽  
Japanese Pine Sawyer

Assessment of Metarhizium anisopliae (Clavicipitaceae) and its vector, Scleroderma guani (Hymenoptera: Bethylidae), for the control of Monochamus alternatus (Coleoptera: Cerambycidae)

2014 ◽  
Vol 147 (5) ◽  
pp. 628-634
Author(s):  
M. Xu ◽  
F.Y. Xu ◽  
Y.P. Liu ◽  
Y.S. Pan ◽  
X.Q. Wu
Keyword(s):  
Metarhizium Anisopliae ◽  
Bursaphelenchus Xylophilus ◽  
Biocontrol Agents ◽  
Lethal Concentration ◽  
Pinewood Nematode ◽  
Intensive Study ◽  
Monochamus Alternatus ◽  
Major Disease ◽  
Larval Parasitoid

AbstractThe pinewood nematode, Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle (Aphelenchida: Parasitaphelenchidae), is a major disease of pines forests in China. Its vector, Monochamus alternatus Hope (Coleoptera: Cerambycidae), has been the focus of an intensive study to improve the efficiency of the parasitoid Scleroderma guani Xiao and Wu (Hymenoptera: Bethylidae) by using the parasitoid both as a larval parasitoid in its own right and additionally as a vector for the entomophagous pathogen Metarhizium anisopliae (Metchnikoff) Sorokin (Clavicipitaceae). Twenty-one M. anisopliae strains were screened and the best four tested for sporulation capabilities at a range of temperatures. The best strain was then evaluated at several sporulation concentrations to define the lethal concentration 50 (LC50) against the larvae of M. alternatus. Scleroderma guani was inoculated with the best strain and tested against larvae of M. alternatus, which were constrained singly in glass vials. Three S. guani females per vial infected with M. anisopliae (strain 789) were shown to cause the death of 100% of the M. alternatus larvae they were exposed to as compared exposures to just one or two infected S. guani females per vial. This demonstrates a successful interaction of two biocontrol agents for the control of the pinewood nematode vector M. alternatus.

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