scholarly journals Nonspectroscopic Migratory Cell Monitoring Method Using Retroreflective Janus Microparticles

ACS Omega ◽  
2020 ◽  
Vol 5 (38) ◽  
pp. 24790-24798
Author(s):  
Ka Ram Kim ◽  
Danbi Lee ◽  
Kwan Young Jeong ◽  
Kyung Won Lee ◽  
Moon Suk Kim ◽  
...  
Keyword(s):  
Monitoring Method ◽  
Cell Monitoring ◽  
Migratory Cell

scholarly journals Single Cell Analysis of Neutrophils NETs by Microscopic LSPR Imaging System

Micromachines ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 52 ◽  
Author(s):  
Riyaz Ahmad Mohamed Ali ◽  
Daiki Mita ◽  
Wilfred Espulgar ◽  
Masato Saito ◽  
Masayuki Nishide ◽  
...  
Keyword(s):  
Single Cell ◽  
High Throughput Screening ◽  
Single Cell Analysis ◽  
Imaging System ◽  
Minimum Cost ◽  
Great Sensitivity ◽  
Monitoring Method ◽  
Cell Monitoring ◽  
Single Cell Isolation ◽  
Significant Release

A simple microengraving cell monitoring method for neutrophil extracellular traps (NETs) released from single neutrophils has been realized using a polydimethylsiloxane (PDMS) microwell array (MWA) sheet on a plasmon chip platform. An imbalance between NETs formation and the succeeding degradation (NETosis) are considered associated with autoimmune disease and its pathogenesis. Thus, an alternative platform that can conduct monitoring of this activity on single cell level at minimum cost but with great sensitivity is greatly desired. The developed MWA plasmon chips allow single cell isolation of neutrophils from 150 µL suspension (6.0 × 105 cells/mL) with an efficiency of 36.3%; 105 microwells with single cell condition. To demonstrate the utility of the chip, trapped cells were incubated between 2 to 4 h after introducing with 100 nM phorbol 12-myristate 13-acetate (PMA) before measurement. Under observation using a hyperspectral imaging system that allows high-throughput screening, the neutrophils stimulated by PMA solution show a significant release of fibrils and NETs after 4 h, with observed maximum areas between 314–758 µm2. An average absorption peak wavelength shows a redshift of Δλ = 1.5 nm as neutrophils release NETs.

IMPROVEMENT OF MONITORING METHOD FOR SPATIAL DISTRIBUTION OF VACANT HOUSES USING MUNICIPAL PUBLIC DATA

2019 ◽  
Vol 84 (764) ◽  
pp. 2165-2174
Author(s):  
Yuki AKIYAMA ◽  
Akihiro UEDA ◽  
Kenta OUCHI ◽  
Natsuki ITO ◽  
Yoshiya ONO ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Monitoring Method ◽  
Public Data
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