scholarly journals Extraction, Characterization, and Anticoagulant Activity of a Sulfated Polysaccharide from Bursatella leachii Viscera

ACS Omega ◽  
2020 ◽  
Vol 5 (24) ◽  
pp. 14786-14795 ◽  
Author(s):  
Manel Dhahri ◽  
Salim Sioud ◽  
Rihab Dridi ◽  
Mohsen Hassine ◽  
Naceur A. Boughattas ◽  
...  
Keyword(s):  
Anticoagulant Activity ◽  
Sulfated Polysaccharide ◽  
Bursatella Leachii

Antioxidant and anticoagulant activity of sulfated polysaccharide from Gracilaria debilis (Forsskal)

2015 ◽  
Vol 81 ◽  
pp. 1031-1038 ◽  
Author(s):  
Sadhasivam Sudharsan ◽  
Namasivayam Subhapradha ◽  
Palaniappan Seedevi ◽  
Vairamani Shanmugam ◽  
Perumal Madeswaran ◽  
...  
Keyword(s):  
Anticoagulant Activity ◽  
Sulfated Polysaccharide

scholarly journals Anti-Thrombin, Anti-Adhesive, Anti-Migratory, and Anti-Proliferative Activities of Sulfated Galactans from the Tropical Green Seaweed, Udotea flabellum

Marine Drugs ◽  
2018 ◽  
Vol 17 (1) ◽  
pp. 5 ◽  
Author(s):  
Maxsuell Mendes Marques ◽  
Fernando Presa ◽  
Rony Viana ◽  
Mariana Costa ◽  
Monica Amorim ◽  
...  
Keyword(s):  
Antitumor Agents ◽  
Anticoagulant Activity ◽  
Sulfated Polysaccharide ◽  
Northeastern Brazil ◽  
Sulfated Galactans ◽  
Green Seaweed ◽  
Coated Surface ◽  
Time Test ◽  
Coated Surfaces

In this study, sulfated polysaccharide-rich extracts were isolated from 22 tropical seaweeds (4 red, 11 brown, and 7 green) found in northeastern Brazil, and evaluated for the role of anticoagulant agents. Fifteen of the extracts showed anticoagulant activity, including all the extracts from green seaweeds. Udotea flabellum (a green seaweed) extract was the most potent, requiring an amount of only 3 µg to double the plasma coagulation time in the activated partial thromboplastin time test. A similar result was obtained with 1 µg of heparin. Two sulfated homogalactans with anticoagulant activity, F-I (130 kDa) and F-II (75 kDa), were isolated from this extract using several bio-guided purification steps. Their anticoagulant activity, as well as properties related to antitumor activity (anti-proliferative, anti-adhesive, and anti-migratory), were accessed. Their anticoagulant activities were close to that of heparin. We found that F-I and F-II (0.5–10 μg/mL) were not able to directly inhibit thrombin. In the presence of anti-thrombin, F-I (0.5 μg/mL) was more effective than heparin (0.5 μg/mL) in inhibiting thrombin, while F-II showed similar effects as heparin. F-I and F-II also inhibited B16-F10 (murine melanoma cells) adhesion, migration, and proliferation on a fibronectin-coated surface, but not on laminin- or collagen I-coated surfaces. Except for the antiproliferative activity, the other effects of F-I and F-II were eliminated upon their desulfation (~50%), indicating that the degree of sulfation is not as important for F-I and F-II anti-proliferative activity as the sulfation position. Taken together, the results provide strong evidence for the potential utility of sulfated galactans from U. flabellum, making these compounds an interesting option for future investigations that aim to design new anticoagulant/antitumor agents.

scholarly journals Anticoagulant Properties of a Green Algal Rhamnan-type Sulfated Polysaccharide and Its Low-molecular-weight Fragments Prepared by Mild Acid Degradation

Marine Drugs ◽  
2018 ◽  
Vol 16 (11) ◽  
pp. 445 ◽  
Author(s):  
Xue Liu ◽  
Peng Du ◽  
Xiao Liu ◽  
Sujian Cao ◽  
Ling Qin ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Anticoagulant Activity ◽  
Sulfated Polysaccharide ◽  
Low Molecular Weight ◽  
Thrombin Time ◽  
Acid Degradation ◽  
Molecular Weights ◽  
Green Algal

The active sulfated polysaccharide from seaweed possesses important pharmaceutical and biomedical potential. In the study, Monostroma sulfated polysaccharide (MSP) was obtained from Monostroma angicava, and the low-molecular-weight fragments of MSP (MSP-Fs: MSP-F1–MSP-F6) were prepared by controlled acid degradation. The molecular weights of MSP and MSP-F1–MSP-F6 were 335 kDa, 240 kDa, 90 kDa, 40 kDa, 24 kDa, 12 kDa, and 6.8 kDa, respectively. The polysaccharides were sulfated rhamnans that consisted of →3)-α-l-Rhap-(1→ and →2)-α-l-Rhap-(1→ units with partial sulfation at C-2 of →3)-α-l-Rhap-(1→ and C-3 of →2)-α-l-Rhap-(1→. Anticoagulant properties in vitro of MSP and MSP-F1–MSP-F6 were evaluated by studying the activated partial thromboplastin time, thrombin time, and prothrombin time. Anticoagulant activities in vivo of MSP and MSP-F4 were further evaluated; their fibrin(ogen)olytic activities in vivo and thrombolytic properties in vitro were also assessed by D-dimer, fibrin degradation products, plasminogen activator inhibitior-1, and clot lytic rate assays. The results showed that MSP and MSP-F1–MSP-F4 with molecular weights of 24–240 kDa had strong anticoagulant activities. A decrease in the molecular weight of MSP-Fs was accompanied by a decrease in the anticoagulant activity, and higher anticoagulant activity requires a molecular weight of over 12 kDa. MSP and MSP-F4 possessed strong anticoagulant activities in vivo, as well as high fibrin(ogen)olytic and thrombolytic activities. MSP and MSP-F4 have potential as drug or helpful food supplements for human health.

scholarly journals Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides

2011 ◽  
Vol 54 (4) ◽  
pp. 691-700 ◽  
Author(s):  
José Ariévilo Gurgel Rodrigues ◽  
Edfranck de Sousa Oliveira Vanderlei ◽  
Érika Façanha Bessa ◽  
Francisco de Araújo Magalhães ◽  
Regina Célia Monteiro de Paula ◽  
...  
Keyword(s):  
Anticoagulant Activity ◽  
Sulfated Polysaccharide ◽  
Green Seaweed

scholarly journals Anticoagulant and Antithrombotic Properties in Vitro and in Vivo of a Novel Sulfated Polysaccharide from Marine Green Alga Monostroma nitidum

Marine Drugs ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 247 ◽  
Author(s):  
Sujian Cao ◽  
Xiaoxi He ◽  
Ling Qin ◽  
Meijia He ◽  
Yajing Yang ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Green Alga ◽  
Anticoagulant Activity ◽  
Sulfated Polysaccharide ◽  
Carotid Artery Thrombosis ◽  
Artery Thrombosis ◽  
Monostroma Nitidum ◽  
Marine Green Alga

Sulfated polysaccharides from marine algae have high potential as promising candidates for marine drug development. In this study, a homogeneous sulfated polysaccharide from the marine green alga Monostroma nitidum, designated MS-1, was isolated using water extraction and anion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that MS-1 mainly consisted of →3)-α-l-Rhap-(1→ and →2)-α-l-Rhap-(1→ residues, with additional branches consisting of 4-linked β-d-xylose, 4-/6-linked d-glucose, terminal β-d-glucuronic acid, and 3-/2-linked α-l-rhamnose. Sulfate ester groups substituted mainly at C-2/C-4 of →3)-α-l-Rhap-(1→ and C-4 of →2)-α-l-Rhap-(1→ residues, slightly at C-2 of terminal β-d-glucuronic residues. MS-1 exhibited strong anticoagulant activity in vitro and in vivo as evaluated by the activated partial thromboplastin time and thrombin time assays, and significantly decreased platelet aggregation. The anticoagulant activity mechanism of MS-1 was mainly attributed to strong potentiation thrombin by heparin cofactor-II, and it also hastened thrombin and coagulation factor Xa inhibitions by potentiating antithrombin-III. MS-1 possessed markedly thrombolytic activity evaluated by plasminogen activator inhibitior-1, fibrin degradation products, and D-dimer levels using rats plasma, and recanalization rate by FeCl3-induced carotid artery thrombosis in mice. MS-1 exhibited strong antithrombotic activity in vitro and in vivo evaluated by the wet weighs and lengths of thrombus, and thrombus occlusion time by electrically-induced carotid artery thrombosis in rats. These results suggested that MS-1 could be a promising marine drug for prevention and therapy of thromboembolic disease.

Anticoagulant activity of sulfated polysaccharide isolated from fermented brown seaweed Sargassum fulvellum

2007 ◽  
Vol 20 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Mahanama De Zoysa ◽  
Chamilani Nikapitiya ◽  
You-Jin Jeon ◽  
Youngheun Jee ◽  
Jehee Lee
Keyword(s):  
Anticoagulant Activity ◽  
Brown Seaweed ◽  
Sulfated Polysaccharide ◽  
Sargassum Fulvellum
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