scholarly journals In Situ Absorption and Fluorescence Microspectroscopy Investigation of the Molecular Incorporation Process into Single Nanoporous Protein Crystals

ACS Omega ◽  
2020 ◽  
Vol 5 (16) ◽  
pp. 9605-9613
Author(s):  
Takayuki Uwada ◽  
Kohei Kouno ◽  
Mitsuru Ishikawa
2019 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Issam Mohammed Abushammala ◽  
Elham Abed Abuwaked ◽  
Hanan Mohammed Fayyad ◽  
Ahmed Fadel Elqedra ◽  
Mai Abdelrahman Ramadan ◽  
...  

1986 ◽  
Vol 38 (S12) ◽  
pp. 37P-37P
Author(s):  
J. Bell ◽  
G. E. Peters ◽  
C. McMartin ◽  
N. W. Thomas ◽  
C. G. Wilson

2019 ◽  
Vol 3 (12) ◽  
pp. 3399-3405
Author(s):  
Jenny Reichert ◽  
Stephanie Maerten ◽  
Katharina Meltzer ◽  
Alexander Tremel ◽  
Manfred Baldauf ◽  
...  

A promising way to increase the methanol yields in CO2 hydrogenation significantly up to 60% by in situ sorption of methanol and water in alkali salt-doped ionic liquids (ILs) is demonstrated.


1992 ◽  
Vol 61 (6) ◽  
pp. 2160-2160
Author(s):  
Toshinori Iwasa ◽  
Tsuyoshi Kawai ◽  
Mitsuyoshi Onoda ◽  
Jyunzo Nakayama ◽  
Hiroo Nakahara ◽  
...  

2010 ◽  
Vol 36 (7) ◽  
pp. 787-794 ◽  
Author(s):  
Yan Xie ◽  
Xiaoli Zeng ◽  
Guowen Li ◽  
Zhenzhen Cai ◽  
Ning Ding ◽  
...  

2017 ◽  
Vol 33 ◽  
pp. 202-210 ◽  
Author(s):  
Álvaro Fernández-Ochoa ◽  
Isabel Borrás-Linares ◽  
Almudena Pérez-Sánchez ◽  
Enrique Barrajón-Catalán ◽  
Isabel González-Álvarez ◽  
...  

1992 ◽  
Vol 81 (10) ◽  
pp. 859-862 ◽  
Author(s):  
T. Pichler ◽  
M. Matus ◽  
J. Kürti ◽  
H. Kuzmany

2011 ◽  
Vol 45 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Yong Yu ◽  
Xuan Wang ◽  
Dominik Oberthür ◽  
Arne Meyer ◽  
Markus Perbandt ◽  
...  

A new crystallization system is described, which makes it possible to use an evaporation-based microfluidic crystallization technique for protein crystallization. The gas and water permeability of the used polydimethylsiloxane (PDMS) material enables evaporation of the protein solution in the microfluidic device. The rates of evaporation are controlled by the relative humidity conditions, which are adjusted in a precise and stable way by using saturated solutions of different reagents. The protein crystals could nucleate and grow under different relative humidity conditions. Using this method, crystal growth could be improved so that approximately 1 mm-sized lysozyme crystals were obtained more successfully than using standard methods. The largest lysozyme crystal obtained reached 1.57 mm in size. The disadvantage of the good gas permeability in PDMS microfluidic devices becomes an advantage for protein crystallization. The radius distributions of aggregrates in the solutions inside the described microfluidic devices were derived fromin situdynamic light scattering measurements. The experiments showed that the environment inside of the microfluidic device is more stable than that of conventional crystallization techniques. However, the morphological results showed that the protein crystals grown in the microfluidic device could lose their morphological stability. Air bubbles in microfluidic devices play an important role in the evaporation progress. A model was constructed to analyze the relationship of the rates of evaporation and the growth of air bubbles to the relative humidity.


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