scholarly journals In Vivo Photoacoustic Lifetime Based Oxygen Imaging with Tumor Targeted G2 Polyacrylamide Nanosonophores

ACS Nano ◽  
2019 ◽  
Vol 13 (12) ◽  
pp. 14024-14032 ◽  
Author(s):  
Janggun Jo ◽  
Chang Heon Lee ◽  
Jeff Folz ◽  
Joel W. Y. Tan ◽  
Xueding Wang ◽  
...  
Keyword(s):  
Oxygen Imaging

CHAPTER 4. Hydrophilic Ir(iii) Complexes for In vitro and In vivo Oxygen Imaging

2018 ◽  
pp. 71-90
Author(s):  
Toshitada Yoshihara ◽  
Yosuke Hirakawa ◽  
Masaomi Nangaku ◽  
Seiji Tobita
Keyword(s):  
Oxygen Imaging

In vivo oxygen imaging using green fluorescent protein

2006 ◽  
Vol 291 (4) ◽  
pp. C781-C787 ◽  
Author(s):  
Eiji Takahashi ◽  
Tomohiro Takano ◽  
Yasutomo Nomura ◽  
Satoshi Okano ◽  
Osamu Nakajima ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Molecular Imaging ◽  
Oxygen Concentration ◽  
Fluorescent Protein ◽  
Red Shift ◽  
Coronary Artery Ligation ◽  
Oxygen Imaging ◽  
Gfp Fluorescence ◽  
Green Fluorescent

In vivo oxygen measurement is the key to understanding how biological systems dynamically adapt to reductions in oxygen supply. High spatial resolution oxygen imaging is of particular importance because recent studies address the significance of within-tissue and within-cell heterogeneities in oxygen concentration in health and disease. Here, we report a new technique for in vivo molecular imaging of oxygen in organs using green fluorescent protein (GFP). GFP-expressing COS-7 cells were briefly photoactivated with a strong blue light while lowering the oxygen concentration from 10% to <0.001%. Red fluorescence (excitation 520–550 nm, emission >580 nm) appeared after photoactivation at <2% oxygen (the red shift of GFP fluorescence). The red shift disappeared after reoxygenation of the cell, indicating that the red shift is stable as long as the cell is hypoxic. The red shift of GFP fluorescence was also demonstrated in single cardiomyocytes isolated from the GFP knock-in mouse (green mouse) heart. Then, we tried in vivo molecular imaging of hypoxia in organs. The red shift could be imaged in the ischemic liver and kidney in the green mouse using macroscopic optics provided that oxygen diffusion from the atmospheric air was prevented. In crystalloid-perfused beating heart isolated from the green mouse, significant spatial heterogeneities in the red shift were demonstrated in the epicardium distal to the coronary artery ligation. We conclude that the present technique using GFP as an oxygen indicator may allow in vivo molecular imaging of oxygen in organs.

Dendrimers with Tetrabenzoporphyrin Cores: Near Infrared Phosphors for in vivo Oxygen Imaging.

ChemInform ◽  
2003 ◽  
Vol 34 (33) ◽  
Author(s):  
Ivo B. Rietveld ◽  
Evelyn Kim ◽  
Sergei A. Vinogradov
Keyword(s):  
Near Infrared ◽  
Oxygen Imaging

Dendrimers with tetrabenzoporphyrin cores: near infrared phosphors for in vivo oxygen imaging

Tetrahedron ◽  
2003 ◽  
Vol 59 (22) ◽  
pp. 3821-3831 ◽  
Author(s):  
Ivo B Rietveld ◽  
Evelyn Kim ◽  
Sergei A Vinogradov
Keyword(s):  
Near Infrared ◽  
Oxygen Imaging

Easily prepared ruthenium-complex nanomicelle probes for two-photon quantitative imaging of oxygen in aqueous media

RSC Advances ◽  
2015 ◽  
Vol 5 (1) ◽  
pp. 291-300 ◽  
Author(s):  
Aamir A. Khan ◽  
Susan K. Fullerton-Shirey ◽  
Scott S. Howard
Keyword(s):  
Cross Section ◽  
Ruthenium Complex ◽  
Quantitative Imaging ◽  
Aqueous Media ◽  
Optical Probes ◽  
Two Photon ◽  
Oxygen Imaging ◽  
Oxygen Sensitive ◽  
Photon Cross Section

Easily prepared, biocompatible, and oxygen-sensitive optical probes with a large two-photon cross-section: towards inexpensive quantitative oxygen imaging in vivo.

Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

1974 ◽  
Vol 32 ◽  
pp. 54-55
Author(s):  
S. Phyllis Steamer ◽  
Rosemarie L. Devine
Keyword(s):  
Structural Changes ◽  
Radiation Treatment ◽  
Arterial Stenosis ◽  
Ultrastructural Changes ◽  
Vascular Effects ◽  
Microvascular Changes ◽  
Surrounding Connective Tissue ◽  
Fission Neutrons ◽  
Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

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