Super-efficient in Vivo Two-Photon Photodynamic Therapy with a Gold Nanocluster as a Type I Photosensitizer

ACS Nano ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. 9532-9544 ◽  
Author(s):  
Rongcheng Han ◽  
Miao Zhao ◽  
Zhiwei Wang ◽  
Helin Liu ◽  
Shengcang Zhu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Type I ◽  
Gold Nanocluster ◽  
Two Photon

scholarly journals Two-Photon Time-Gated In Vivo Imaging of Dihydrolipoic-Acid-Decorated Gold Nanoclusters

Materials ◽  
2021 ◽  
Vol 14 (24) ◽  
pp. 7744
Author(s):  
Ye Tian ◽  
Ming Wei ◽  
Lijun Wang ◽  
Yuankai Hong ◽  
Dan Luo ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Gold Nanoclusters ◽  
Photon Absorption ◽  
Gold Nanocluster ◽  
Time Resolved ◽  
Dihydrolipoic Acid ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Time Resolved Imaging

Due to the unique advantages of two-photon technology and time-resolved imaging technology in the biomedical field, attention has been paid to them. Gold clusters possess excellent physicochemical properties and low biotoxicity, which make them greatly advantageous in biological imaging, especially for in vivo animal imaging. A gold nanocluster was coupled with dihydrolipoic acid to obtain a functionalized nanoprobe; the material displayed significant features, including a large two-photon absorption cross-section (up to 1.59 × 105 GM) and prolonged fluorescence lifetime (>300 ns). The two-photon and time-resolution techniques were used to perform cell imaging and in vivo imaging.

scholarly journals Can nanotechnology potentiate photodynamic therapy?

2012 ◽  
Vol 1 (2) ◽  
pp. 111-146 ◽  
Author(s):  
Ying-Ying Huang ◽  
Sulbha K. Sharma ◽  
Tianhong Dai ◽  
Hoon Chung ◽  
Anastasia Yaroslavsky ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Photodynamic Therapy ◽  
Plasmonic Enhancement ◽  
Gold And Silver Nanoparticles ◽  
Drug Delivery Vehicles ◽  
Two Photon ◽  
Photon Excitation ◽  
Poorly Soluble ◽  
Delivery Vehicles

AbstractPhotodynamic therapy (PDT) uses the combination of nontoxic dyes and harmless visible light to produce reactive oxygen species that can kill cancer cells and infectious microorganisms. Due to the tendency of most photosensitizers (PS) to be poorly soluble and to form nonphotoactive aggregates, drug-delivery vehicles have become of high importance. The nanotechnology revolution has provided many examples of nanoscale drug-delivery platforms that have been applied to PDT. These include liposomes, lipoplexes, nanoemulsions, micelles, polymer nanoparticles (degradable and nondegradable), and silica nanoparticles. In some cases (fullerenes and quantum dots), the actual nanoparticle itself is the PS. Targeting ligands such as antibodies and peptides can be used to increase specificity. Gold and silver nanoparticles can provide plasmonic enhancement of PDT. Two-photon excitation or optical upconversion can be used instead of one-photon excitation to increase tissue penetration at longer wavelengths. Finally, after sections on in vivo studies and nanotoxicology, we attempt to answer the title question, “can nanotechnology potentiate PDT?”

scholarly journals Multifunctional AIE iridium (III) photosensitizer nanoparticles for two-photon-activated imaging and mitochondria targeting photodynamic therapy

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Xuzi Cai ◽  
Kang-Nan Wang ◽  
Wen Ma ◽  
Yuanyuan Yang ◽  
Gui Chen ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Water Solubility ◽  
Tissue Imaging ◽  
Ros Generation ◽  
Generation Efficiency ◽  
Deep Tissue ◽  
Two Photon ◽  
Mitochondria Targeting

AbstractDeveloping novel photosensitizers for deep tissue imaging and efficient photodynamic therapy (PDT) remains a challenge because of the poor water solubility, low reactive oxygen species (ROS) generation efficiency, serve dark cytotoxicity, and weak absorption in the NIR region of conventional photosensitizers. Herein, cyclometalated iridium (III) complexes (Ir) with aggregation-induced emission (AIE) feature, high photoinduced ROS generation efficiency, two-photon excitation, and mitochondria-targeting capability were designed and further encapsulated into biocompatible nanoparticles (NPs). The Ir-NPs can be used to disturb redox homeostasis in vitro, result in mitochondrial dysfunction and cell apoptosis. Importantly, in vivo experiments demonstrated that the Ir-NPs presented obviously tumor-targeting ability, excellent antitumor effect, and low systematic dark-toxicity. Moreover, the Ir-NPs could serve as a two-photon imaging agent for deep tissue bioimaging with a penetration depth of up to 300 μm. This work presents a promising strategy for designing a clinical application of multifunctional Ir-NPs toward bioimaging and PDT.

scholarly journals Sonodynamic therapy in combination with photodynamic therapy shows enhanced long-term cure of brain tumor

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ballav M. Borah ◽  
Joseph Cacaccio ◽  
Farukh A. Durrani ◽  
Wiam Bshara ◽  
Steven G. Turowski ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Brain Tumor ◽  
Synergetic Effect ◽  
In Vivo Studies ◽  
Treatment Modalities ◽  
Type I ◽  
Sonodynamic Therapy ◽  
Biological Substrates

AbstractThis article presents the construction of a multimodality platform that can be used for efficient destruction of brain tumor by a combination of photodynamic and sonodynamic therapy. For in vivo studies, U87 patient-derived xenograft tumors were implanted subcutaneously in SCID mice. For the first time, it has been shown that the cell-death mechanism by both treatment modalities follows two different pathways. For example, exposing the U87 cells after 24 h incubation with HPPH [3-(1′-hexyloxy)ethyl-3-devinyl-pyropheophorbide-a) by ultrasound participate in an electron-transfer process with the surrounding biological substrates to form radicals and radical ions (Type I reaction); whereas in photodynamic therapy, the tumor destruction is mainly caused by highly reactive singlet oxygen (Type II reaction). The combination of photodynamic therapy and sonodynamic therapy both in vitro and in vivo have shown an improved cell kill/tumor response, that could be attributed to an additive and/or synergetic effect(s). Our results also indicate that the delivery of the HPPH to tumors can further be enhanced by using cationic polyacrylamide nanoparticles as a delivery vehicle. Exposing the nano-formulation with ultrasound also triggered the release of photosensitizer. The combination of photodynamic therapy and sonodynamic therapy strongly affects tumor vasculature as determined by dynamic contrast enhanced imaging using HSA-Gd(III)DTPA.

scholarly journals Imaging Collagen in Intact Viable Healthy and Atherosclerotic Arteries Using Fluorescently Labeled CNA35 and Two-Photon Laser Scanning Microscopy

2007 ◽  
Vol 6 (4) ◽  
pp. 7290.2007.00021 ◽  
Author(s):  
Remco T.A. Megens ◽  
Mirjam G.A. oude Egbrink ◽  
Jack P.M. Cleutjens ◽  
Marijke J.E. Kuijpers ◽  
Paul H.M. Schiffers ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Apolipoprotein E ◽  
Carotid Arteries ◽  
Ex Vivo ◽  
Atherosclerotic Plaques ◽  
Type I ◽  
Two Photon ◽  
Tunica Adventitia ◽  
Molecular Imaging Agent

We evaluated CNA35 as a collagen marker in healthy and atherosclerotic arteries of mice after both ex vivo and in vivo administration and as a molecular imaging agent for the detection of atherosclerosis. CNA35 conjugated with fluorescent Oregon Green 488 (CNA35/OG488) was administered ex vivo to mounted viable muscular (uterine), elastic (carotid), and atherosclerotic (carotid) arteries and fresh arterial rings. Two-photon microscopy was used for imaging. CNA35/OG488 labeling in healthy elastic arteries was compared with collagen type I, III, and IV antibody labeling in histologic sections. For in vivo labeling experiments, CNA35/OG488 was injected intravenously in C57BL6/J and apolipoprotein E−/− mice. Ex vivo CNA35/OG488 strongly labeled collagen in the tunica adventitia, media, and intima of muscular arteries. In healthy elastic arteries, tunica adventitia was strongly labeled, but labeling in tunica media and intima was prevented by endothelium and elastic laminae. Histology confirmed the affinity of CNA35 for type I, III, and IV collagen in arteries. Strong CNA35/OG488 labeling was found in atherosclerotic plaques. In vivo applied CNA35/OG488 minimally labeled the tunica intima of healthy carotid arteries. Atherosclerotic plaques in apolipoprotein E−/− mice exhibited large uptake. CNA35/OG488 imaging in organs revealed endothelium as a limiting barrier for in vivo uptake. CNA35/OG488 is a good molecular imaging agent for atherosclerosis.

scholarly journals Recent Emergence of Rhenium(I) Tricarbonyl Complexes as Photosensitisers for Cancer Therapy

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4176
Author(s):  
Hui Shan Liew ◽  
Chun-Wai Mai ◽  
Mohd Zulkefeli ◽  
Thiagarajan Madheswaran ◽  
Lik Voon Kiew ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Photophysical Properties ◽  
Two Photon ◽  
Alternative Approach ◽  
Recent Emergence ◽  
Combination Study ◽  
Basic Features ◽  
New Generation

Photodynamic therapy (PDT) is emerging as a significant complementary or alternative approach for cancer treatment. PDT drugs act as photosensitisers, which upon using appropriate wavelength light and in the presence of molecular oxygen, can lead to cell death. Herein, we reviewed the general characteristics of the different generation of photosensitisers. We also outlined the emergence of rhenium (Re) and more specifically, Re(I) tricarbonyl complexes as a new generation of metal-based photosensitisers for photodynamic therapy that are of great interest in multidisciplinary research. The photophysical properties and structures of Re(I) complexes discussed in this review are summarised to determine basic features and similarities among the structures that are important for their phototoxic activity and future investigations. We further examined the in vitro and in vivo efficacies of the Re(I) complexes that have been synthesised for anticancer purposes. We also discussed Re(I) complexes in conjunction with the advancement of two-photon PDT, drug combination study, nanomedicine, and photothermal therapy to overcome the limitation of such complexes, which generally absorb short wavelengths.

scholarly journals NIR Photosensitizer for Two-Photon Fluorescent Imaging and Photodynamic Therapy of Tumor

2021 ◽  
Vol 9 ◽  
Author(s):  
Lujia Chen ◽  
Meijuan Chen ◽  
Yuping Zhou ◽  
Changsheng Ye ◽  
Ruiyuan Liu
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
Deep Tissue ◽  
Two Photon ◽  
Nir Emission ◽  
Good Biocompatibility ◽  
Fluorescent Agent

Preparation of near-infrared (NIR) emissive fluorophore for imaging-guided PDT (photodynamic therapy) has attracted enormous attention. Hence, NIR photosensitizers of two-photon (TP) fluorescent imaging and photodynamic therapy are highly desirable. In this contribution, a novel D-π-A structured NIR photosensitizer (TTRE) is synthesized. TTRE demonstrates near-infrared (NIR) emission, good biocompatibility, and superior photostability, which can act as TP fluorescent agent for clear visualization of cells and vascular in tissue with deep-tissue penetration. The PDT efficacy of TTRE as photosensitizer is exploited in vitro and in vivo. All these results confirm that TTRE would serve as potential platform for TP fluorescence imaging and imaging-guided photodynamic therapy.

Sign in / Sign up

Export Citation Format

Share Document