Probing and Controlling Dynamic Interactions at Biomolecule–Nanoparticle Interfaces Using Stochastic DNA Walkers

ACS Nano ◽  
2019 ◽  
Vol 13 (7) ◽  
pp. 8106-8113 ◽  
Author(s):  
Sean D. Mason ◽  
Guan A. Wang ◽  
Peng Yang ◽  
Yongya Li ◽  
Feng Li
Keyword(s):  
Dynamic Interactions ◽  
Dna Walkers

Solving the mechanisms responsible for organelle behavior in vivo by same-cell correlative light and electron microscopy

1992 ◽  
Vol 50 (1) ◽  
pp. 14-15
Author(s):  
Conly L. Rieder
Keyword(s):  
Electron Microscopy ◽  
Quantitative Analysis ◽  
Light Microscopy ◽  
Living Cell ◽  
Light And Electron Microscopy ◽  
Time Behavior ◽  
Dynamic Interactions ◽  
Positive Identification ◽  
Cellular Components

The behavior of many cellular components, and their dynamic interactions, can be characterized in the living cell with considerable spatial and temporal resolution by video-enhanced light microscopy (video-LM). Indeed, under the appropriate conditions video-LM can be used to determine the real-time behavior of organelles ≤ 25-nm in diameter (e.g., individual microtubules—see). However, when pushed to its limit the structures and components observed within the cell by video-LM cannot be resolved nor necessarily even identified, only detected. Positive identification and a quantitative analysis often requires the corresponding electron microcopy (EM).

Updated understanding of platelets in thrombosis and hemostasis: the roles of integrin PSI domains and their potential as therapeutic targets

2020 ◽  
Vol 20 ◽  
Author(s):  
Daniel Thomas MacKeigan ◽  
Tiffany Ni ◽  
Chuanbin Shen ◽  
Tyler William Stratton ◽  
Wenjing Ma ◽  
...  
Keyword(s):  
Redox Reactions ◽  
Platelet Adhesion ◽  
Adaptive Mechanism ◽  
Dynamic Interactions ◽  
Surface Receptors ◽  
Isomerase Activity ◽  
Pathological Conditions ◽  
Fibrinogen Binding ◽  
Immune Mediated ◽  
Novel Target

: Platelets are small blood cells known primarily for their ability to adhere and aggregate at injured vessels to arrest bleeding. However, when triggered under pathological conditions, the same adaptive mechanism of platelet adhesion and aggregation may cause thrombosis, a primary cause of heart attack and stroke. Over recent decades, research has made considerable progress in uncovering the intricate and dynamic interactions that regulate these processes. Integrins are heterodimeric cell surface receptors expressed on all metazoan cells that facilitate cell adhesion, movement, and signaling, to drive biological and pathological processes such as thrombosis and hemostasis. Recently, our group discovered that the plexinsemaphorin-integrin (PSI) domains of the integrin β subunits exert endogenous thiol isomerase activity derived from their two highly conserved CXXC active site motifs. Given the importance of redox reactions in integrin activation and its location in the knee region, this PSI domain activity may be critically involved in facilitating the interconversions between integrin conformations. Our monoclonal antibodies against the β3 PSI domain inhibited its thiol isomerase activity and proportionally attenuated fibrinogen binding and platelet aggregation. Notably, these antibodies inhibited thrombosis without significantly impairing hemostasis or causing platelet clearance. In this review, we will update mechanisms of thrombosis and hemostasis including platelet versatilities and immune-mediated thrombocytopenia, discuss critical contributions of the newly discovered PSI domain thiol isomerase activity, and its potential as a novel target for anti-thrombotic therapies and beyond.

UNDERSTANDING THE DYNAMIC INTERACTIONS BETWEEN EARTH-SURFACE PROCESSES AND TECTONICS: OPPORTUNITIES FOR PROGRESS FROM OUTCROP TO GLOBAL SCALES

2017 ◽  
Author(s):  
Katharine W. Huntington ◽  
◽  
Keith Klepeis ◽  
Elizabeth J. Cassel ◽  
Claire A. Currie ◽  
...  
Keyword(s):  
Surface Processes ◽  
Earth Surface ◽  
Dynamic Interactions

Toughness

2009 ◽  
pp. 536-548
Author(s):  
Richard A. Dienstbier ◽  
Lisa M. Pytlik Zillig
Keyword(s):  
The Body ◽  
Positive Outcomes ◽  
Adaptive Coping ◽  
Dynamic Interactions ◽  
Abstract Level ◽  
The Central Nervous System ◽  
Cortical System ◽  
The Mind ◽  
And Training ◽  
Neuroendocrine Systems

This chapter presents an overview of the concept of toughness, which at the abstract level is about the harmony of physiological systems, and more concretely is about how the body influences the mind. Toughness theory begins with the recognition that there is a “training effect” for neuroendocrine systems. Following a review of the characteristics of interventions and training programs that can promote toughness, the authors present a model in which the effects of toughness are mediated by neuroendocrine systems such as the pituitary-adrenal-cortical system and the central nervous system. The elements of toughness (e.g., having a greater capacity for arousal and energy when needed) are proposed to promote positive outcomes by facilitating the use of adaptive coping strategies and improving emotional stability. Toughness therefore appears to be a promising concept within positive psychology in that it helps to explain how the dynamic interactions between psychological and somatic processes can promote positive outcomes.

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