Preferential Binding of Cytochrome c to Anionic Ligand-Coated Gold Nanoparticles: A Complementary Computational and Experimental Approach

ACS Nano ◽  
2019 ◽  
Vol 13 (6) ◽  
pp. 6856-6866 ◽  
Author(s):  
Emily J. Tollefson ◽  
Caley R. Allen ◽  
Gene Chong ◽  
Xi Zhang ◽  
Nikita D. Rozanov ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cytochrome C ◽  
Experimental Approach ◽  
Anionic Ligand ◽  
Preferential Binding

Self-assembled multilayer of gold nanoparticles for amplified electrochemical detection of cytochrome c

The Analyst ◽  
2008 ◽  
Vol 133 (9) ◽  
pp. 1242 ◽  
Author(s):  
Jing Zhao ◽  
Xiaoli Zhu ◽  
Ting Li ◽  
Genxi Li
Keyword(s):  
Gold Nanoparticles ◽  
Cytochrome C ◽  
Electrochemical Detection ◽  
Self Assembled

scholarly journals Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles

Sensors ◽  
2018 ◽  
Vol 19 (1) ◽  
pp. 40 ◽  
Author(s):  
Chenxing Guo ◽  
Jianfang Wang ◽  
Xianzhe Chen ◽  
Yujiao Li ◽  
Lifang Wu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cytochrome C ◽  
Electrochemical Reduction ◽  
Direct Electrochemistry ◽  
High Sensitivity ◽  
Carbon Electrode ◽  
Graphene Oxides ◽  
Deposition Amount ◽  
Reduced Graphene ◽  
Cyt C

A biosensor based on a combination of cytochrome c (Cyt c), electrochemical reduced graphene oxides (ERGO), and gold nanoparticles (AuNPs) on a glassy carbon electrode (GCE) was fabricated. The proposed biosensor electrode was denoted as GCE/ERGO-Nafion/AuNPs/Cyt c/Nafion, where ERGO-Nafion was deposited by dropping graphene oxides-Nafion mixed droplet first and following electrochemical reduction, AuNPs were directly deposited on the surface of the ERGO-Nafion modified electrode by electrochemical reduction, and other components were deposited by the dropping-dry method. The effect of the deposition amount of AuNPs on direct electrochemistry of Cyt c in the proposed electrode was investigated. The hydrogen peroxide was taken to evaluate the performance of the proposed biosensor. The results showed that the biosensor has great analytical performance, including a high sensitivity, a wide linear range, a low detection limit, and good stability, reproducibility, and reliability.

Surface selective binding of 2,5-dimercapto-1,3,4-thiadiazole (DMTD) on silver and gold nanoparticles: a Raman and DFT study

RSC Advances ◽  
2016 ◽  
Vol 6 (67) ◽  
pp. 62529-62539 ◽  
Author(s):  
Nandita Maiti ◽  
Ridhima Chadha ◽  
Abhishek Das ◽  
Sudhir Kapoor
Keyword(s):  
Gold Nanoparticles ◽  
Au Nanoparticles ◽  
Dft Study ◽  
Dft Studies ◽  
Selective Binding ◽  
Silver And Gold Nanoparticles ◽  
Preferential Binding

SERS and DFT studies indicated preferential binding of DMTD towards Ag and Au nanoparticles through ring N and thiocarbonyl S atoms.

scholarly journals Interactions of Peptide Coated Gold Nanoparticles with Spike Protein of the SARS-CoV-2: A Basis for Design of a Simple and Rapid Detection Tool

2020 ◽  
Author(s):  
Nitu Verma ◽  
Yogesh Badhe ◽  
Rakesh Gupta ◽  
Auhin Maparu ◽  
Beena Rai
Keyword(s):  
Gold Nanoparticles ◽  
Host Cell ◽  
Potential Of Mean Force ◽  
Spike Protein ◽  
Coarse Grained ◽  
Dynamic Simulations ◽  
Host Cell Membrane ◽  
Preferential Binding ◽  
Set Up ◽  
Force Calculation

<p>The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic which not only created a situation of dealing with public health emergency but also triggered the financial crisis of international concern. The current situation demands rapid, convenient and reliable diagnosis of the disease to downregulate its spread. Primary method of diagnosis presently being used, such as nucleic acid testing (RT-PCR), CT scans etc. involve time-consuming advanced machinery for imaging/ RNA replication and highly skilled technicians which could be only done in a laboratory set-up. A rapid, simple yet selective naked eye detection methodology that does not require any advanced instrumental techniques is highly desirable. </p> <p>In this study, we report computational results which could form the basis of a simple and rapid strategy for the detection of SARS-Cov-2 using peptide (screened from angiotensin-converting enzyme 2 (ACE2) receptor of host cell) functionalized gold nanoparticles (GNPs). This is based on the preferential binding of viral spike (S) protein to ACE2 receptor situated on the surface of the host cell membrane by which the virus gains access to the host cell. The interaction of peptide coated GNPs with spike protein has been investigated using coarse grained molecular dynamic simulations. The potential of mean force calculation of spike protein confirmed strong binding between peptide and receptor binding domain (RBD) of spike protein. The results presented here demonstrate the potential of this peptide coated GNPs-based system in the development of convenient sensors for the clinical diagnosis. </p>

Optical Changes and Writing on Hydrotalcite Supported Gold Nanoparticles

2008 ◽  
Vol 8 (1) ◽  
pp. 274-279
Author(s):  
Leonardo S. Bonifacio ◽  
Claudia R. Gordijo ◽  
Vera R. L. Constantino ◽  
Denise O. Silva ◽  
Pedro K. Kiyohara ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Plasmon Resonance ◽  
Binding Sites ◽  
Thermally Induced ◽  
Color Changes ◽  
Preferential Binding ◽  
Supported Gold Nanoparticles ◽  
Supported Gold ◽  
Microscopy Techniques ◽  
Aggregation Phenomenon

Gold nanoparticles (AuNP) incorporated into hydrotalcite (HT), provide an interesting type of pigment in which temperature can modulate the plasmon resonance and the aggregation phenomenon. As inferred from microscopy techniques, the preferential binding sites are located at the border of the HT external basal surface, leading to aggregates of gold nanoparticles displaying characteristic plasmon resonance and interference bands around 520 and 700 nm, respectively. The thermally induced color changes in the HT-supported gold material arise from the competition between of nanoparticles aggregation and fusion processes, as characterized by TEM and STM. A laser beam can also induce such changes, allowing the writing of optical information on this type of material.

A miniaturized electrochemical assay for homocysteine using screen-printed electrodes with cytochrome c anchored gold nanoparticles

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 6071-6078 ◽  
Author(s):  
Thangamuthu Madasamy ◽  
Christian Santschi ◽  
Olivier J. F. Martin
Keyword(s):  
Gold Nanoparticles ◽  
Cytochrome C ◽  
Blood Plasma ◽  
Point Of Care ◽  
Plasma Samples ◽  
Electrochemical Assay ◽  
Screen Printed Electrodes ◽  
Screen Printed

Electrochemical point-of-care analysis of homocysteine in a drop of the blood plasma samples.

Biomineralization of Gold Nanoparticles by Lysozyme and Cytochrome c and Their Applications in Protein Film Formation

Langmuir ◽  
2010 ◽  
Vol 26 (16) ◽  
pp. 13535-13544 ◽  
Author(s):  
Mandeep Singh Bakshi ◽  
Harpreet Kaur ◽  
Tarlok Singh Banipal ◽  
Narpinder Singh ◽  
Gurinder Kaur
Keyword(s):  
Gold Nanoparticles ◽  
Cytochrome C ◽  
Film Formation ◽  
Protein Film
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