Single Particle Tracking and Super-Resolution Imaging of Membrane-Assisted Stop-and-Go Diffusion and Lattice Assembly of DNA Origami

ACS Nano ◽  
2019 ◽  
Author(s):  
Susanne Kempter ◽  
Alena Khmelinskaia ◽  
Maximilian T. Strauss ◽  
Petra Schwille ◽  
Ralf Jungmann ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Super Resolution ◽  
Single Particle Tracking ◽  
Dna Origami ◽  
Stop And Go ◽  
Resolution Imaging

scholarly journals Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D

2019 ◽  
Vol 7 ◽  
Author(s):  
Jeanne Linarès-Loyez ◽  
Joana S. Ferreira ◽  
Olivier Rossier ◽  
Brahim Lounis ◽  
Gregory Giannone ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Super Resolution ◽  
Single Particle Tracking ◽  
Resolution Imaging

scholarly journals VMD as a Software for Visualization and Quantitative Analysis of Super Resolution Imaging and Single Particle Tracking

2014 ◽  
Vol 106 (2) ◽  
pp. 202a ◽  
Author(s):  
Yanxin Liu ◽  
John E. Stone ◽  
En Cai ◽  
Jingyi Fei ◽  
Sang Hak Lee ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Particle Tracking ◽  
Single Particle ◽  
Super Resolution ◽  
Single Particle Tracking ◽  
Resolution Imaging

scholarly journals Photoactivation of silicon rhodamines via a light-induced protonation

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Michelle S. Frei ◽  
Philipp Hoess ◽  
Marko Lampe ◽  
Bianca Nijmeijer ◽  
Moritz Kueblbeck ◽  
...  
Keyword(s):  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Super Resolution ◽  
Spectroscopic Properties ◽  
Single Particle Tracking ◽  
Live Cell ◽  
Localization Microscopy ◽  
Super Resolution Microscopy ◽  
Single Molecule Localization Microscopy

Abstract Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

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