scholarly journals Label-Free Quantification of Small-Molecule Binding to Membrane Proteins on Single Cells by Tracking Nanometer-Scale Cellular Membrane Deformation

ACS Nano ◽  
2018 ◽  
Vol 12 (2) ◽  
pp. 2056-2064
Author(s):  
Fenni Zhang ◽  
Wenwen Jing ◽  
Ashley Hunt ◽  
Hui Yu ◽  
Yunze Yang ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Small Molecule ◽  
Single Cells ◽  
Cellular Membrane ◽  
Nanometer Scale ◽  
Label Free ◽  
Membrane Deformation ◽  
Label Free Quantification ◽  
Free Quantification

scholarly journals Kinetics of small molecule interactions with membrane proteins in single cells measured with mechanical amplification

2015 ◽  
Vol 1 (9) ◽  
pp. e1500633 ◽  
Author(s):  
Yan Guan ◽  
Xiaonan Shan ◽  
Fenni Zhang ◽  
Shaopeng Wang ◽  
Hong-Yuan Chen ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Small Molecule ◽  
Single Cells ◽  
Cellular Membrane ◽  
Mechanical Amplification ◽  
New Strategy ◽  
Interaction Kinetics ◽  
Molecule Interactions ◽  
Difficult Challenge ◽  
Membrane Protein Interaction

Measuring small molecule interactions with membrane proteins in single cells is critical for understanding many cellular processes and for screening drugs. However, developing such a capability has been a difficult challenge. We show that molecular interactions with membrane proteins induce a mechanical deformation in the cellular membrane, and real-time monitoring of the deformation with subnanometer resolution allows quantitative analysis of small molecule–membrane protein interaction kinetics in single cells. This new strategy provides mechanical amplification of small binding signals, making it possible to detect small molecule interactions with membrane proteins. This capability, together with spatial resolution, also allows the study of the heterogeneous nature of cells by analyzing the interaction kinetics variability between different cells and between different regions of a single cell.

scholarly journals LB1116 Label-free quantification of the pharmacokinetics of small molecule drugs in skin

2019 ◽  
Vol 139 (9) ◽  
pp. B18
Author(s):  
A. Feizpour ◽  
L. Bastholm ◽  
T. Marstrand ◽  
S. Eirefelt ◽  
C.L. Evans
Keyword(s):  
Small Molecule ◽  
Label Free ◽  
Label Free Quantification ◽  
Small Molecule Drugs ◽  
Free Quantification

scholarly journals Spectral Clustering Improves Label-Free Quantification of Low-Abundant Proteins

2019 ◽  
Vol 18 (4) ◽  
pp. 1477-1485 ◽  
Author(s):  
Johannes Griss ◽  
Florian Stanek ◽  
Otto Hudecz ◽  
Gerhard Dürnberger ◽  
Yasset Perez-Riverol ◽  
...  
Keyword(s):  
Spectral Clustering ◽  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification

Global Analysis of Protein Expression in A549 Cells After Prolonged Nicotine Exposure by Using Label-free Quantification

2021 ◽  
Vol 41 (8) ◽  
pp. 3833-3842
Author(s):  
SASIKARN KOMKLEOW ◽  
CHURAT WEERAPHAN ◽  
DARANEE CHOKCHAICHAMNANKIT ◽  
PAPADA CHAISURIYA ◽  
CHRIS VERATHAMJAMRAS ◽  
...  
Keyword(s):  
Protein Expression ◽  
Global Analysis ◽  
A549 Cells ◽  
Nicotine Exposure ◽  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification

Label-Free Quantification of Anticancer Drug Imatinib in Human Plasma with Surface Enhanced Raman Spectroscopy

2018 ◽  
Vol 90 (21) ◽  
pp. 12670-12677 ◽  
Author(s):  
Stefano Fornasaro ◽  
Alois Bonifacio ◽  
Elena Marangon ◽  
Mauro Buzzo ◽  
Giuseppe Toffoli ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Human Plasma ◽  
Anticancer Drug ◽  
Surface Enhanced Raman Spectroscopy ◽  
Label Free ◽  
Label Free Quantification ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Free Quantification

Label-free quantification of asymmetric cancer-cell filopodium activities in a multi-gradient chip

Lab on a Chip ◽  
2009 ◽  
Vol 9 (7) ◽  
pp. 884 ◽  
Author(s):  
Tsi-Hsuan Hsu ◽  
Meng-Hua Yen ◽  
Wei-Yu Liao ◽  
Ji-Yen Cheng ◽  
Chau-Hwang Lee
Keyword(s):  
Cancer Cell ◽  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification

scholarly journals Improved Intensity-Based Label-Free Quantification via Proximity-Based Intensity Normalization (PIN)

2014 ◽  
Vol 13 (3) ◽  
pp. 1281-1292 ◽  
Author(s):  
Susan K. Van Riper ◽  
Ebbing P. de Jong ◽  
LeeAnn Higgins ◽  
John V. Carlis ◽  
Timothy J. Griffin
Keyword(s):  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification ◽  
Intensity Normalization

scholarly journals Assessment of Label-Free Quantification in Discovery Proteomics and Impact of Technological Factors and Natural Variability of Protein Abundance

2017 ◽  
Vol 16 (4) ◽  
pp. 1410-1424 ◽  
Author(s):  
MHD Rami Al Shweiki ◽  
Susann Mönchgesang ◽  
Petra Majovsky ◽  
Domenika Thieme ◽  
Diana Trutschel ◽  
...  
Keyword(s):  
Natural Variability ◽  
Protein Abundance ◽  
Label Free ◽  
Technological Factors ◽  
Label Free Quantification ◽  
Free Quantification

scholarly journals StPeter: Seamless Label-Free Quantification with the Trans-Proteomic Pipeline

2018 ◽  
Vol 17 (3) ◽  
pp. 1314-1320 ◽  
Author(s):  
Michael R. Hoopmann ◽  
Jason M. Winget ◽  
Luis Mendoza ◽  
Robert L. Moritz
Keyword(s):  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification
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