Thermoresponsive Gel Embedded with Adipose Stem-Cell-Derived Extracellular Vesicles Promotes Esophageal Fistula Healing in a Thermo-Actuated Delivery Strategy

ACS Nano ◽  
2018 ◽  
Vol 12 (10) ◽  
pp. 9800-9814 ◽  
Author(s):  
Amanda K. A. Silva ◽  
Silvana Perretta ◽  
Guillaume Perrod ◽  
Laetitia Pidial ◽  
Véronique Lindner ◽  
...  
Keyword(s):  
Stem Cell ◽  
Extracellular Vesicles ◽  
Esophageal Fistula ◽  
Adipose Stem Cell ◽  
Delivery Strategy ◽  
Fistula Healing ◽  
Thermoresponsive Gel

scholarly journals PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Tatiana Lopatina ◽  
Enrica Favaro ◽  
Cristina Grange ◽  
Massimo Cedrino ◽  
Andrea Ranghino ◽  
...  
Keyword(s):  
Stem Cell ◽  
Protective Effect ◽  
Extracellular Vesicles ◽  
Hindlimb Ischemia ◽  
Adipose Stem Cell

scholarly journals Screening and Functional Pathway Analysis of Pulmonary Genes Associated with Suppression of Allergic Airway Inflammation by Adipose Stem Cell-Derived Extracellular Vesicles

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Sung-Dong Kim ◽  
Shin Ae Kang ◽  
Yong-Wan Kim ◽  
Hak Sun Yu ◽  
Kyu-Sup Cho ◽  
...  
Keyword(s):  
Stem Cell ◽  
Airway Inflammation ◽  
Extracellular Vesicles ◽  
Pathway Analysis ◽  
Kegg Pathway ◽  
Allergic Airway Inflammation ◽  
Formyl Peptide Receptor ◽  
Paraoxonase 1 ◽  
Adipose Stem Cell ◽  
Kegg Pathway Analysis

Background. Although mesenchymal stem cell- (MSC-) derived extracellular vesicles (EVs) are as effective as MSCs in the suppression of allergic airway inflammation, few studies have explored the molecular mechanisms of MSC-derived EVs in allergic airway diseases. The objective of this study was to evaluate differentially expressed genes (DEGs) in the lung associated with the suppression of allergic airway inflammation using adipose stem cell- (ASC-) derived EVs. Methods. C57BL/6 mice were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged intranasally with OVA. To evaluate the effect of ASC-derived EVs on allergic airway inflammation, 10 μg/50 μL of EVs were administered intranasally prior to OVA challenge. Lung tissues were removed and DEGs were compared pairwise among the three groups. DEG profiles and hierarchical clustering of the identified genes were analyzed to evaluate changes in gene expression. Real-time PCR was performed to determine the expression levels of genes upregulated after treatment with ASC-derived EVs. Enrichment analysis based on the Gene Ontology (GO) database and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were also performed to further identify the function of DEGs. Results. Expression of paraoxonase 1 (PON1), brain-expressed X-linked 2 (Bex2), insulin-like growth factor binding protein 6 (Igfbp6), formyl peptide receptor 1 (Fpr1), and secretoglobin family 1C member 1 (Scgb1c1) was significantly increased in asthmatic mice following treatment with ASC-derived EVs. GO enrichment and KEGG pathway analysis showed that these genes were strongly associated with immune system processes and their regulation, cellular processes, single-organism processes, and biological regulation. Conclusion. These results suggest that the DEGs identified in this study (PON1, Bex2, Igfbp6, Fpr1, and Scgb1c1) may be involved in the amelioration of allergic airway inflammation by ASC-derived EVs.

scholarly journals Adipose Stem Cell-Derived Extracellular Vesicles Induce Proliferation of Schwann Cells via Internalization

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 163 ◽  
Author(s):  
Maximilian Haertinger ◽  
Tamara Weiss ◽  
Anda Mann ◽  
Annette Tabi ◽  
Victoria Brandel ◽  
...  
Keyword(s):  
Image Analysis ◽  
Stem Cell ◽  
Extracellular Vesicles ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Automated Image Analysis ◽  
Dependent Manner ◽  
Adipose Stem Cell ◽  
Silica Beads

Recent studies showed a beneficial effect of adipose stem cell-derived extracellular vesicles (ADSC-EVs) on sciatic nerve repair, presumably through Schwann cell (SC) modulation. However, it has not yet been elucidated whether ADSC-EVs exert this supportive effect on SCs by extracellular receptor binding, fusion to the SC membrane, or endocytosis mediated internalization. ADSCs, ADSC-EVs, and SCs were isolated from rats and characterized according to associated marker expression and properties. The proliferation rate of SCs in response to ADSC-EVs was determined using a multicolor immunofluorescence staining panel followed by automated image analysis. SCs treated with ADSC-EVs and silica beads were further investigated by 3-D high resolution confocal microscopy and live cell imaging. Our findings demonstrated that ADSC-EVs significantly enhanced the proliferation of SCs in a time- and dose-dependent manner. 3-D image analysis revealed a perinuclear location of ADSC-EVs and their accumulation in vesicular-like structures within the SC cytoplasm. Upon comparing intracellular localization patterns of silica beads and ADSC-EVs in SCs, we found striking resemblance in size and distribution. Live cell imaging visualized that the uptake of ADSC-EVs preferentially took place at the SC processes from which the EVs were transported towards the nucleus. This study provided first evidence for an endocytosis mediated internalization of ADSC-EVs by SCs and underlines the therapeutic potential of ADSC-EVs in future approaches for nerve regeneration.

Immune Reprogramming Precision Photodynamic Therapy of Peritoneal Metastasis by Scalable Stem-Cell-Derived Extracellular Vesicles

ACS Nano ◽  
2021 ◽  
Vol 15 (2) ◽  
pp. 3251-3263
Author(s):  
Amandine Pinto ◽  
Iris Marangon ◽  
Julie Méreaux ◽  
Alba Nicolás-Boluda ◽  
Grégory Lavieu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Photodynamic Therapy ◽  
Extracellular Vesicles ◽  
Peritoneal Metastasis

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

PROTEOMICS ◽  
2021 ◽  
pp. 2000098
Author(s):  
Annalisa L.E. Carli ◽  
Shoukat Afshar‐Sterle ◽  
Alin Rai ◽  
Haoyun Fang ◽  
Ryan O'Keefe ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Stem Cell Marker ◽  
Cancer Stem Cell Marker ◽  
Cell Marker ◽  
Gastric Cancer Cells ◽  
Migratory Phenotype
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